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Citations for Bio-Rad :
5751 - 5800 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... samples were resuspended in ultrapure water and protein concentrations were determined by Bradford assay (BioRad). To generate nuclear extracts ...
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bioRxiv - Plant Biology 2022Quote: ... total protein content in the extracts was quantified with a Bradford assay (Bio-Rad; 5000006). The assay was performed using 50 μg of total protein extract and 300 μM of substrate in assay buffer (100mM HEPES-KOH pH 7.8 ...
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bioRxiv - Biophysics 2022Quote: ... and using a fast protein liquid chromatography (FPLC) system (NGC Chromatography System Quest 10, BioRad).
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein was resolved in 4-20% precast polyacrylamide gel (Bio-Rad, USA). For immunoprecipitation ...
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bioRxiv - Biochemistry 2022Quote: ... Protein bands on the membrane were detected using the ChemiDoc Touch Imaging System (Bio-Rad) after incubation with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot® TurboTM (Biorad) system ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amounts of proteins were resolved on 4%–20% gradient Tris-glycine gels (Bio-Rad) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... 60-80 µg proteins were subjected into SDS polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and transferred to PVDF membranes (Bio-rad) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 μg of protein was resolved in 4%-12% Bis/Tris-acrylamide gradient gels (BioRad) and transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was collected for measuring protein concentration using a Bradford reagent (Biorad, cat # 5000001). Samples were denatured in NuPAGE® LDS sample buffer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: The concentrations of the 37°C samples were determined with a DC Protein Assay (BioRad). A volume corresponding to 50 μg of the 37°C sample was used for further analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Total lysate containing the equivalent of 1.1 mg of protein (Easy Bradford BioRad, BSA standards) was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... The protein sample was separated on 4-12% Criterion-XT Bis-Tris gels (Bio-Rad). For SDS-PAGE experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was then concentrated and analyzed by SDS-PAGE reducing gels (Bio-Rad) (Figure S7A) ...
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bioRxiv - Bioengineering 2022Quote: ... Protein samples were separated on SDS– PAGE gel and transferred to PVDF membrane (BioRad, USA). Membranes were incubated in blocking buffer (Tris-buffered salinewith 0.1% Tween 20 (TBS-T ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein transfer to PVDF membranes was performed using the Trans-Blot Turbo Transfer System (BioRad) or standard manual transferring techniques ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were run on 4-15% Mini protean TGX stain-free gels (Bio-Rad) at 100V for 4h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein was transferred to nitrocellulose membrane via semi-dry method (Bio-Rad Trans-Blot Turbo) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... ECL solution (Amersham) was used for detection of protein levels in ChemiDoc XRS system (Biorad).
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bioRxiv - Cell Biology 2022Quote: ... Chemiluminescence signals of the proteins were detected with Clarity ECL substrate solution (BIORAD, US, 1705061) by Vilber Loumart FX-7 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with ClarityTM Western ECL Substrate (Bio-Rad #170-5061) on the ChemiDoc Touch System (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amounts of proteins were loaded and separated on 12% Criterion Precast Gel (Bio-Rad), subsequently transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Proteins were then electro-transferred onto Trans Blot Turbo polyvinylidene fluoride membranes (Bio-Rad Laboratories). Western blots were probed with rabbit anti-human c-Rel antibody (Cell Signaling) ...
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bioRxiv - Immunology 2020Quote: ... A total of 50μg protein was diluted 1:1 with 2x Laemmli buffer (Bio-Rad), resolved by SDS-PAGE and transferred into PVDF membranes ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... samples were run in a 4-20% Protean TGX Precast protein gels (Biorad, CA, USA). To transfer proteins ...
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bioRxiv - Developmental Biology 2021Quote: ... The amount of protein in each lysate were quantified using the DC Lowry assay (BioRad) according to manufacturer’s instruction.
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bioRxiv - Biochemistry 2020Quote: ... The 3 mg/mL protein sample was injected onto NGC Liquid Chromotography System (Bio-Rad) equipped with a HiPrep Sephacryl S-500 HR column equilibrated in FPLC buffer and separated into 156 ...
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bioRxiv - Genomics 2020Quote: ... Protein lysates (20-30ug) were then used for running using 10-15% gradient gels (Biorad) for 1.5 hours at 100-110V followed by dry-transfer to PVDF membrane (Biorad dry transfer) ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 mL of 100 μM protein samples was loaded on ENrich SEC 70 column (BioRad) in a buffer containing 50 mM phosphates (pH 7.4) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to PVDF membrane using the Trans-Blot Turbo Blotting System (Bio-Rad). Primary antibody incubation was overnight at 4°C (anti-RIF1 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by protein isolation and quantification with a Bradford assay (Bio-Rad, Hercules, CA, USA) (Bradford ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of proteins were separated via SDS/PAGE using TGX precast gels (Bio-Rad), transferred to nitrocellulose membranes using a TransBlot Turbo System (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of the protein bands was carried out using a GS-800 Calibrated Densitometer (BioRad) and the BioRad software Quantity One 4.2.3.
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a nitrocellulose membrane using a Trans-Blot Turbo system (Bio-Rad), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein lysates were separated by electrophoresis over 4–15% mini-Protean TGX gels (Bio-Rad) and transferred to 0.45µm Immobilon-FL polyvinylidene fluoride membrane (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins extracts(ca.20μg) were resolved by SDS-PAGE (4 - 15 % Mini-PROTEAN TGX, Biorad) and transferred onto a nitrocellulose membrane and were blocked according to appropriate primary antibodies Membranes were visualized using an Odyssey® DLx scanner using Odyssey® imaging software ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were diluted (3:1) with a 4x Laemmli sample buffer (Biorad, Cat# 1610747) and heated at 98 °C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated in 10% polyacrylamide gels (Mini-PROTEAN TGX Stain-Free Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Protein concentrations were measured after sonication by the bicinchoninic acid assay (Bio-Rad, Hercules, CA), and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343 ...
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bioRxiv - Biochemistry 2021Quote: Purified protein was mixed in 1:1 ratio with 2X laemmli loading dye (Bio-rad) prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble proteins were extracted by using Profinity IMAC Ni-charged resin (Bio-Rad, Hercules, CA) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... The extracted lysates and histone proteins were resolved on a 4-20% gradient gel (Biorad) and a 15% polyacrylamide gel ...
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bioRxiv - Microbiology 2021Quote: ... Protein detection was performed using Clarity and Clarity Max ECL Western Blotting Substrates (Bio-Rad) on a Bio-Rad ChemiDoc Imaging System.
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bioRxiv - Bioengineering 2021Quote: ... Protein concentrations were measured and subjected to SDS-polyacrylamide gel electrophoresis (Bio-Rad, Hercules, CA) at 4 to 20% ...
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bioRxiv - Neuroscience 2020Quote: ... Chemo-luminescent detection of protein bands was performed at the blot documentation system (ChemDoc, BioRad) after applying the HRP substrate solution (Serva) ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by SDS-PAGE on 4-20% Criterion TGX precast gels (Bio-Rad), then transferred to nitrocellulose membrane (0.2 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were then prepared in Laemmli Sample Buffer (Bio-Rad, Hercules, CA; Cat #1610747) with 2.5% 2-Mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were separated using 4-20% SDS-PAGE and transferred onto nitrocellulose membrane (Bio-Rad). Membrane was blocked in 5% non-fat dry milk in 1x TBST ...
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bioRxiv - Physiology 2022Quote: ... Proteins were separated on Any kD Criterion TGX Stain-Free gels (Bio-Rad, Hercules, CA) at 75V for 150 minutes in running buffer (Cell Signaling) ...
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bioRxiv - Neuroscience 2022Quote: ... MAGL activity probe bands and MAGL protein intensity bands were quantified using ImageLab (Bio-Rad). TAMRA-FP selectivity studies were normalized to Coomassie Brilliant Blue band intensity ...