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Citations for Bio-Rad :
5501 - 5550 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Protein transfer to the membrane was performed on ice in a MiniProtean chamber (BioRad) that was filled with transfer buffer at 300 mA for 90 min.
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bioRxiv - Neuroscience 2023Quote: Protein samples were resolved by electrophoresis on 4-12% Bis-Tris gels (Bio-Rad) and were transferred to polyvinylidene difluoride membranes using the iBlot system (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was concentrated and loaded on the Enrich SEC 650 10/300 (Bio-rad) size exclusion column and the labeling was monitored with absorption at 280 and 490 nm ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were then transferred onto nitrocellulose membranes using the turbo transfer system (BIO-RAD). Membranes were saturated with TBS with 0.5% Tween-20 and 5% Milk for 1 hour prior to overnight 4°C incubation with the indicated primary antibody diluted 1/1000° in TBS-Tween-5%BSA 0.025% Sodium Azide ...
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bioRxiv - Neuroscience 2023Quote: ... Molecular markers of known size (Precision Plus Protein™ Dual Color Standard, Bio-Rad) were run in parallel with the proteins of interest ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then separated on 4-15% mini protean TGX precast gels (Bio-Rad) in running buffer (200 mM glycine ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was determined by Bradford assay according to manufacturer’s instructions (Bio-Rad Laboratories). Protein samples were denatured (95 °C for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred to a methanol activated (10s) PVDF membrane (Bio-Rad 1620177) at 100V for 1hr in 1X Tris-Glycine buffer (Bio-Rad 161-0734 ...
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bioRxiv - Immunology 2023Quote: 10 ug of the protein lysate were mixed with 4X Laemmli buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... Protein samples were boiled with 4x Laemmli sample buffer (Bio-Rad, cat no. 1610747), with 10% b-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to nitrocellulose membranes with Trans-Blot Turbo transfer system (Bio-Rad) using Mini TGX gel transfer protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46; Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated by SDS-PAGE using home-made or precast gels (Bio-Rad) with suitable percentage then transferred on nitrocellulose membranes (GE Healthcare or Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA). 2.5 μM CsoSCA was mixed with 0.5 μM Rubisco or 5 μM CsoS1A and CsoS1B and incubated for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... 30 μg total protein was then diluted in Laemmli Sample Buffer (Bio-Rad, 1610747) and analysed by SDS-PAGE using Mini-PROTEAN TGX Gels 4-20% (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... The residual protein extracts were mixed with 4X Laemmli sample buffer (BIO-RAD, #1610747) at a ratio of 1:3 ...
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bioRxiv - Immunology 2023Quote: ... Total proteins were visualized as internal control using stain free reagent in ChemiDoc (BioRad) with a 5 min exposition time and then transferred on 0.22 µm PVDF membrane (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein was visualized after staining with Oriole fluorescent gel stain solution (Bio-Rad) for 1.5-2 h.
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bioRxiv - Neuroscience 2023Quote: ... the pulled down proteins were electrophoresed on 4-20 % precast polyacrylamide gel (BIO-RAD) for 1 h with 100 fixed voltage and transferred to PVDF membranes using wet method for 90 min with100 fixed voltage ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were visualized by chemiluminescence using Immun-Star(tm) AP substrate (Bio-Rad) applied to the membrane and incubation for 5 min at ambient temperature ...
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bioRxiv - Physiology 2023Quote: ... signal intensity of each protein was measured with ImageLab software (Bio-Rad, v. 6.0) and normalized to levels of ⍺-tubulin or total protein (Ponceau S) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein were loaded on 4-15% SDS-PAGE gel (Bio-Rad) and the gel was run at 55 mA for 50 min ...
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bioRxiv - Immunology 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad). Membranes were probed using goat anti-mouse LDLR at a 1:500 dilution (AF2255 ...
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bioRxiv - Neuroscience 2023Quote: ... and electrophoresed in 4–20% Mini Protean TGX precast protein gels (4561093, Bio-Rad), followed by transferring to a 45⍰mm nitrocellulose membrane (1620115 ...
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Astrocytic Neuroligins Are Not Required for Synapse Formation or a Normal Astrocyte CytoarchitecturebioRxiv - Neuroscience 2023Quote: ... Proteins were transferred from gels to 0.2 µm nitrocellulose membranes (Bio-Rad, Cat# 1620112) using the Trans-blot turbo system (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein band densities were analyzed with Image Lab software (Bio-Rad Laboratories, Watford, UK).
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membranes (Bio-Rad). Mono/polyclonal primary antibodies and appropriate fluorescent-conjugated secondary antibodies were used for blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by Quick Start™ Bradford 1x Dye Reagent (Bio-Rad). Equal amounts of proteins were reduced with 1x loading buffer containing 6% β-mercaptoethanol and resolved on 10% SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted on nitrocellulose membranes (Cytiva) using a wet transfer system (Bio-Rad). Membranes were blocked in blocking solution (5% w/v non-fat milk in PBS-Tween 0.1% v/v) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein expression was detected by chemiluminescence in the ChemiDoc imaging system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad). Primary antibodies and appropriate horseradish peroxidase (HRP)- conjugated secondary antibodies (Jackson Immuno Research Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded onto a 4-20% Criterion TGX Precast Protein Gel (Bio-Rad)7 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were resolved in 4-15% pre-cast SDS-PAGE (Biorad cat n° 4561085) at 80V for two hours and transferred to PVDF membrane (Immobilon-P ...
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bioRxiv - Neuroscience 2023Quote: ... Protein signal was detected using enhanced chemiluminescence (ClarityTM Western ECL Substrate, 170-5060, Biorad) and images were taken using the Chemidoc Touch Imaging System (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... then equal amounts of protein per sample were separated by SDS-PAGE (Bio-Rad) and transferred to PVDF membranes (Immobilon) ...
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bioRxiv - Immunology 2023Quote: ... Equal protein amounts were loaded and separated on 4%– 15% pre-cast gels (BioRad) and transferred using a Trans-Blot turbo system (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... Co-IPs were performed by using the magnetic Sure Beads Protein A from BioRad washed with dilution buffer (10mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... 20-50µg of protein was loaded onto a stain-free 4-15% gel (BioRad), the Rubisco large subunit was visualized using stain-free gel imaging ...
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bioRxiv - Neuroscience 2023Quote: ... 30 μg of proteins were boiled in Laemmli sample buffer (Bio-Rad, Hercules, CA) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by Mini-Protean TGX stain-free gels 4-20% (BioRad 4568093) and transferred to TransBlot Turbo mini-size PVDF membrane (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: Proteins were resolved by Mini-Protean TGX stain-free gels 4-20% (BioRad 4568093) and transferred to TransBlot Turbo mini-size PVDF membrane (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Separated proteins were transferred to a nitrocellulose membrane using a semi-dry system (BioRad), and immunoblot analysis was performed with the indicated antibodies ...
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bioRxiv - Biochemistry 2023Quote: Proteins were transferred to nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Biorad) and incubated with the indicated primary antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein contents were equilibrated using stain-free 4%-15% gels (Bio-Rad) and then quickly transferred to Nitrocellulose using a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined using a standard Bradford Colorimetric Assay kit (Bio-Rad, 5000116).
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bioRxiv - Cell Biology 2023Quote: ... Same protein amount per sample was diluted with Laemmli sample buffer (4X; BioRad, 1610791) supplemented with 10% 2-betamercaptoethanol (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... to image relative protein amounts using a ChemiDoc Gel Imaging System (BioRad, Hercules, CA). After acquisition ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto nitrocellulose membranes using a semi-dry transfer system (BioRad). The membranes were blocked in 5% milk in TBS-T before incubation with primary antibodies overnight at 4°C in 3% milk or BSA in TBS-T ...