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Citations for Bio-Rad :
5501 - 5550 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... For each sample 50 μg of protein were incubated at 95°C for 10 min and then loaded into a gradient (4-15%) western blot gel (mini-protein TGX gel, Bio-Rad, Milan, Italy). Proteins were transferred onto a 0.45 μm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: The FPLC-size exclusion chromatography analysis was performed by injecting samples of heme-treated oligomers1 and oligomers2 onto a Bio-Rad ENrich SEC70 10 x 300 mm column using the Bio-Rad NGC FPLC system (Bio-Rad, CA, USA). The retention time of both oligomer types was compared with a set of molecular weight protein standards (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: Twenty-five micrograms of each renal cortex fraction was added to 10% or 12% polyacrylamide gels (Mini-protean TGX precast gels, Bio-rad, CA, USA), and assessed using infrared secondary probes (LICOR ...
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bioRxiv - Microbiology 2019Quote: ... 60 μg of protein extract for each strain analyzed was loaded into a 10% TGX Stain-Free™ FastCast™ Acrylamide gel (Bio-Rad). Separated proteins were transferred onto a nitrocellulose membrane using the Trans-Blot® Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: All analyses estimated using auto-sampling a 10 μl to fractionated in an Aminex HPX-87H column (Bio-Rad Laboratories, Hercules, CA, USA), analyzed in a refractive index detector (RID-10A ...
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bioRxiv - Microbiology 2021Quote: ... and phospho-Akt (p-Akt) were quantified in 10 μg of total protein using the Bio-Plex Pro magnetic phosphoprotein detection assay (Bio-Rad, United Kingdom). Phosphoprotein expression was normalized to β-actin and expressed as fold-change relative to the uninfected (UI ...
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bioRxiv - Microbiology 2021Quote: Droplet Digital PCR (ddPCR) via the Bio-Rad QX200 platform used 20 µl reactions with 10 µl 2x QX200 ddPCR EvaGreen Supermix (Biorad, Hercules, CA, US), 0.4 µM of forward and reverse primer ...
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bioRxiv - Bioengineering 2019Quote: ... Proteins (15 μg each) were separated based on their molecular weight with SDS–PAGE on 10% Mini-PROTEAN gels (Bio-Rad, Hercules, CA) and then transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2020Quote: Equal amounts of each rgp120 preparation (10 µg) were separated on a 4-15% Mini-PROTEAN TGX precast gel (Bio-Rad Laboratory Inc.) under denaturing and reducing conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA corresponding to approximately 10 ng of starting RNA was used and qPCR was performed with SYBR green master mix (Bio-Rad Laboratories, Inc.). Primers for real-time PCR were GAPDH-F ...
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bioRxiv - Biophysics 2020Quote: ... placed on a heat block (+90 °C) for 10 min and sonicated before separation by SDS-PAGE (4-20% Mini-PROTEAN TGX Gels, Bio-Rad, 456-1096). Next ...
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bioRxiv - Cell Biology 2021Quote: ... Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab, California, USA). The proteins transferred to the membrane were visualized by Ponceau solution stain 0.3% for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... the samples were cooled on ice for 10 min followed by the addition of 80 mg Bio-beads SM-2 Resin (Bio-Rad, Hercules, CA), then rotated at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty micrograms of proteins for each well were separated on 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (cat. num. 161-0374; Bio-Rad, Hercules, CA) and transferred to a polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: ... Twenty μg of proteins were separated on 10% SDS-polyacrylamide gels and transferred onto polyvinylidene difluoride (PVDF) membranes in a Trans-Blot apparatus (Trans-Blot Turbo™, Bio-Rad, USA). Membranes were then saturated with a buffer containing 5% milk ...
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bioRxiv - Molecular Biology 2020Quote: ... Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was performed on 10% gels using the BioRad Mini-Protean Tetra Electrophoresis System (Bio-Rad, CA, USA). The gel was stained with Coomassie brilliant blue R-250 and was scanned on a gel imager (Bio-Rad Laboratories (Shanghai ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 10 µg pEGFP-EB3 (kindly provided by Linda Wordeman, Seattle, WA, USA) using a GenePulser Xcell (Bio-Rad Laboratories, USA) at 500 µF and 300 V (HCT116 ...
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bioRxiv - Microbiology 2021Quote: ... 10 µg of the linearized construct was used for the electroporation of 1×108 procyclic promastigotes (twice at 25 µF and 1500V with 10s interval) using the Bio-Rad GenePulse Xcell electroporation unit (Bio-rad Laboratories, USA). MIL-SPpyRE9/DsRed and MIL-RPpyRE9/DsRed transfectants were selected under Nourseothricin (Jena Bioscience ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 90°C and loaded on a 4–20% (w/v) Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad). The proteins were separated by electrophoresis and blotted onto a membrane with trans-Blot Turbo Mini 0.2 µm Nitrocellulose Transfer Packs (Bio-Rad) ...
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bioRxiv - Epidemiology 2019Quote: Genomic DNA was individually extracted using the modified Chelex resin 10% extraction protocol (Resin Chelex100 ®, Chelating Ion Exchange Resin, Bio-Rad, France) described in [40–42] ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the bound dye was re-dissolved in 10 mM Tris base solution for OD determination at 565 nm using a microplate reader (Bio-Rad 680, America).
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bioRxiv - Microbiology 2019Quote: ... to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm; Bio-Rad, Hercules, CA). As negative controls ...
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bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Cell Biology 2019Quote: ... A WT culture synchronized for 5% ring stages was loaded with 100 µg vector in transfection buffer via electroporation (310 V, 950 µF, 10 ms; Bio-Rad gene-pulser) as described (Ngwa et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Pathology 2019Quote: ... DNA from each specimen was pre-amplified for 10 cycles before subsequent droplet generation using the QX200 Droplet Generator (Bio-Rad Laboratories, USA)[25] ...
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bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
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bioRxiv - Biochemistry 2021Quote: ... protein samples were boiled for 5 min in 1x Laemmli sample buffer and separated on an SDS-PAGE using Mini-PROTEAN® TGXTM Precast Protein Gels or 10–20% Mini-PROTEAN® Tris-Tricine Gels (Bio-Rad). Blotting was performed on a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... total and surface labeled proteins were separated using a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (Bio-Rad, Feldkirchen, Germany). The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789 ...
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bioRxiv - Microbiology 2019Quote: ... All qPCR reactions were performed in volumes of 20 μL composed of 10 μL of IQ™ SYBR® Green Supermix (Bio-Rad), 0.2 μL of each primer at 50 μM ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were set up using the following volumes: 10 µL 2x ddPCR Supermix for probes (no dUTP) (BioRad Laboratories© 186-3024), 5 µL of 20plex oligo mix ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad, USA) and resolved at 200 volts for 30-35 minutes ...
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bioRxiv - Genetics 2022Quote: ... Aliquots were resolved in 10-12% SDS-polycrylamide gels and transferred to nitrocellulose membranes with a Trans-Blot Turbo Transfer System (Bio-Rad, Madrid, Spain) for blotting ...
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bioRxiv - Physiology 2022Quote: Protein samples from the left ventricular heart wall or from injected Xenopus oocytes were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The primary ...
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bioRxiv - Microbiology 2022Quote: ... Insoluble material was then removed by centrifugation at 14,000 x g for 10 min and the supernatant was loaded on a 12% Mini-Protean TGX SDS-PAGE system (Bio-Rad; CA, USA). After staining with Coomassie Blue (ThermoScientific ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg (PrrF1, PrrF2) or 20 µg (PrrH) of total RNA was electrophoresed on a 10% denaturing urea TBE gel (Bio-Rad, Hercules, CA). The RNA was then transferred to a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 min at 70°C and then loaded on a 12% Mini-protean TGX gel (Bio-Rad Cat# 4561044). The gels were run for approximately 1 h at 100 V ...