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Citations for Bio-Rad :
5551 - 5600 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Three ddPCR reactions were set up for each of the mRNA fusion assays with the following volumes: 10 µL 2x ddPCR Supermix for Probes (no dUTP) (PN 186-3023, Bio-Rad, Hercules, CA), 1 µL 20x mRNA Fusions Assay ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was collected and samples were run on 10% or 15% SDS-PAGE and analyzed by western blot using Clarity Western ECL Blotting Substrates (Bio-Rad Laboratories Inc.) and imaged using a ChemiDoc MP (Bio-Rad Laboratories Inc.) ...
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bioRxiv - Genetics 2023Quote: ... was equilibrated in Milli-Q purified H2O for 10 min and then placed in the Bio-Dot Microfiltration Apparatus (Bio-Rad, 170-6545) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Some of the affinity chromatography-purified proteins were run through a Superdex200 Increase 10/300 GL column integrated in an NGC chromatography system (Bio-Rad, CA, USA) to obtain a profile of the content in different species (aggregates ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Developmental Biology 2023Quote: Proteins from worm lysates or coIP experiments were separated on 10% or 4-20% gradient Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Inc.) at 300V ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Bioengineering 2024Quote: ... were separated on a 4–10% gradient sodium dodecyl sulfate-polyacrylamide gel at 120 V for 90 min using a PowerPac Basic setup (Bio-Rad Laboratories, Inc.). Next ...
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bioRxiv - Cell Biology 2024Quote: RNA isolation and Northern blot analysis from human cultured fibroblasts were performed as previously described[82] using 10 micrograms of RNA per lane and analyzed by phosphorimager quantitation (Bio-Rad, Hercules, CA) of the resulting autoradiograph of the Northern blot (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 15 μl of homogenized lysate was used for SDS–polyacrylamide gel electrophoresis (Mini-PROTEAN® TGX™ Precast Gels, 10 wells, Bio-Rad). Proteins were transferred to a Hybond nitrocellulose membrane (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of purified genomic DNA was added to a reaction consisting of 10 µL of ddPCR Supermix for Probes (Bio-Rad, Cat #1863024), 1 µL of MseI (New England Biolabs ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were performed in a volume of 20 µL consisting of 10 µL of 2x iTAQ universial SYBR Green supermix (Bio-Rad Laboratories, USA), 2.0 µL DNA template ...
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bioRxiv - Biochemistry 2024Quote: ... Reagents on plates were gently mixed by rotational agitation for 10 min before measuring the absorbance at 570 nm using a microplate reader (Bio-Rad, 680 XR).
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bioRxiv - Genomics 2021Quote: ... at 4°C in 1×TAE buffer (40 mM Tris-acetate, 1 mM EDTA) using 0.55% Certified Megabase agarose gel (Bio-Rad, 161-3109). Short-range PFGE ran at 4.2 or 4.5 V/cm with a pulse time from 40 to 70 s for 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated for 1 h at RT with goat anti-Lipid A LPS antibodies (diluted 1:1,000 in blocking buffer; Bio-Rad; Cat # OBT1844). Blots were washed three times with TBST (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were probed for 1 hour at room temperature with 1:500 anti-XLF(New England Peptides, details in Methods under Immunodepletion) or 1:1000 anti-His (Bio-Rad MCA1396A) in PBST with 2.5% BSA ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked in 5% dry non-fat milk in PBST and probed using PRP8a antibody (1:500) and an HRP coupled anti-Rabbit secondary antibody (Biorad, 1:10000). All antibodies were diluted in 1% dry nonfat milk in PBST ...
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bioRxiv - Microbiology 2019Quote: ... pH 8 for 1 h at 37°C and then loaded into the wells of 1% pulse-field certified agarose gel (Bio-Rad Laboratories) in 0.5X TBE (45mM Tris HCl ...
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bioRxiv - Bioengineering 2020Quote: ... and annealed from 90°C to 20°C at 1 °C min-1 in a T100™ Thermal Cycler (Bio-Rad, USA). Following construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Table 1) was performed at 4°C overnight at a 1:1000 dilution in 5% nonfat dry milk (Bio-Rad No. 1706404). Incubation of HRP conjugated secondary antibody (different species ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... All cDNA samples were diluted 1:5 and 1 μL was used in quantitative PCR (qPCR) reactions with iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124), according to manufacturer’s instruction and using the Applied Biosystems QuantStudio 7 flex qPCR System ...
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bioRxiv - Molecular Biology 2023Quote: ... The HIV-1 copy number was determined by a total HIV-1 DNA assay on droplet digital PCR (Bio-Rad, QX200 system), as described previously (28) ...
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bioRxiv - Biochemistry 2023Quote: ... different amounts of TTR1st in the range 0.1 1−1.10 μg were loaded in a native 4-15% PAGE gel (Mini-PROTEAN® TGX™ Precast Gel, Bio-Rad). The gel contained colored molecular weight standards (Precision Plus Protein Dual Color Standards ...
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bioRxiv - Immunology 2023Quote: ... The samples and a protein ladder (a 1:1 mix of All Blue Prestained Protein Standards: Unstained Protein Standards; Precision Plus Protein™, Bio-Rad) were loaded to 4-15% mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... A 1:1 Langmuir binding model was used to fit the data measured on a ProteOn™ XPR36 instrument (Bio-Rad Laboratories) using ProteOn™ Manager Software (Version 3.1.0.6 ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was incubated with secondary antibodies for 1 h at room temperature: goat anti-rabbit IgG (H+L)-HRP conjugate (170-6510, Biorad, 1:10000) and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cancer Biology 2021Quote: ... molten 1% certified low melt agarose (Bio-Rad, 1613112) in PBS was equilibrated to 45°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by instillation of 1% low melting agarose (BioRad) through the trachea to prevent leakage of dispase ...
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bioRxiv - Cell Biology 2020Quote: ... Sheep anti-TGN46 from Biorad (AHP500, Immunofluorescence 1:500), Hoechst 33342 from Invitrogen Molecular Probes (H3570).
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bioRxiv - Immunology 2021Quote: ... CD68 (1:200; Bio-Rad, cat no. MCA1957, monoclonal), and C1q (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and CD11b (1:500, #MCA275R, Bio-Rad, CA, USA). Samples were washed with 1X PBS and incubated with Alexa fluorophore-conjugated secondary antibodies (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... and StarBright Blue 700 anti-rabbit (1:5.000, BioRad) in TBS-T containing 5% milk powder ...
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bioRxiv - Molecular Biology 2020Quote: ... rat polyclonal anti-α-tubulin (1:5000, Bio-rad), mouse monoclonal anti-Flag M2 (1:2000 ...
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bioRxiv - Genomics 2022Quote: ... gel was prepared in 1× TAE buffer (BIO-RAD). DNA samples were loaded together with Quick-Load 1 kb Extend DNA Ladder (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... and Hoechst 33342 (1:10000, Bio-Rad, Cat. # 151304) for 1 hour.
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bioRxiv - Molecular Biology 2019Quote: ... rabbit polyclonal anti-GAPDH (Bio-Rad, 1:1,000, VPA00187), and mouse monoclonal anti-HA (Sigma ...
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bioRxiv - Neuroscience 2019Quote: Sheep anti-GFP (1:500) (Bio-rad, #4745-1051), Rabbit anti-RFP (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 1:500 Rat anti-C-Myc (BioRad, Hercules, CA), Mouse anti-COMMD1 (Novus Biologicals ...
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bioRxiv - Immunology 2021Quote: ... in 1 × Tris/Glycine buffer (Bio-Rad, # 161-0734) at 70□V for 60□min ...
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bioRxiv - Microbiology 2021Quote: ... 40 mg ml−1 blotting-grade blocker (Bio-Rad), and 5% (v/v ...