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Citations for Sino Biological :
801 - 831 of 831 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Peripheral blood mononuclear cells (PBMCs) were suspended in ImmunoCult™-XF T Cell Expansion Medium supplemented with 10 μg/ml CD3Ab (OKT3, obtained from Sino Biological, Beijing, China). Subsequently ...
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bioRxiv - Cancer Biology 2020Quote: ... The resultant fluorescent PAA-nanopatterns of Fab anti-Fc antibody fragment protein were incubated overnight with 1 mg/mL ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times and then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... HIV-1 Gag p24 or actin using anti-V5 (for pseudovirion detection; V2660)/anti-S2 (for virion detection; Sino Biologicals; 40590-T62), anti-N (Sino Biologicals ...
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bioRxiv - Immunology 2021Quote: ... the viral nucleocapsid was detected using 1:5000 of SARS-CoV/SARS-CoV-2 Nucleocapsid monoclonal antibody (Sino Biological, Cat#40143-R001) by incubation at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated in a primary mouse SARS-CoV-2 nucleocapsid (N) antibody (Sino Biological Inc., Cat # 40588-T62, 1:1000) at 4 °C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... Amount of Tf in plaque homogenates was also determined by western blot analysis using the anti-Tf antibody (1:2000, 11019-RP01, Sino Biological Inc, China) as described above.
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bioRxiv - Microbiology 2021Quote: ... Then the sections were incubated with primary antibodies: rabbit anti-SARS-CoV Spike (1:200, 40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse anti-SARS-CoV NP (1:200 ...
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bioRxiv - Immunology 2021Quote: ... When this was done the mix was marked with rabbit monoclonal antibody anti-SARS-CoV-2 S1 (1:200) (cat. n° 40150-R007, Sino Biological, Beijing, China) as primary antibody for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and incubated the monolayer with the rabbit polyclonal antibody specific to SARS-CoV-2 RBD protein (1:200) (cat. n° 40592-T62, Sino Biological, Beijing, China), and a chicken antiserum specific to Newcastle disease virus (1:200 ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (pH 6.0) for 12 min for SARS-CoV-1 nucleoprotein antibody (Sino Biological Inc.; Beijing, China) and using recombinant protease from Streptomyces griseus (PanReac Applichem ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded lung were incubated for 1 hour with the primary antibodies SARS-CoV-2 nucleoprotein (mouse IgG1, catalog number 40143-MM08, Sino Biological, Wayne, PA), Ionized calcium-binding adaptor (IBA1 ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Pathology 2020Quote: ... HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc., North Wales, PA) and selecting using the addition of 100 μg/mL hygromycin ...
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bioRxiv - Immunology 2021Quote: ... were fixed after 48 hrs with 1:1 solution of methanol/ acetone and air-dried prior to immunostaining with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62; Sino Biological; 1:1000 dilution) and HRP-conjugated anti-Rabbit IgG antibody (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% skim milk in PBS-T and incubated with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62, Sino Biological; 1:1,000 dilution) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Then the sections were incubated with primary antibodies: rabbit polyclonal anti-SARS-CoV Spike at a dilution of 1:200 (40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse monoclonal anti-SARS-CoV NP at a dilution of 1:200 (40143-MM05 ...
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bioRxiv - Immunology 2021Quote: ... and blotted onto nitrocellulose membranes which were probed with the SARS-CoV2/2019-nCoV Spike rabbit polyclonal Ab (PAb, cat# 40591-T62, Sino Biologicals; dilution 1:1,000) followed by anti-Rabbit IgG-HRP conjugated antibody (1:15,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... followed by rabbit monoclonal antibody against SARS-CoV2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Microbiology 2021Quote: ... and 10% FBS in 0.1% Tween-20 PBS (PBST) for 1 h prior to staining with a rabbit polyclonal anti SARS-CoV-2 nucleocapsid antibody (Sino Biological Inc. catalog # 40588-T62) diluted 1:500 and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Mice serum was 3-fold diluted starting from 1:15 (Omicron) or 1:45 (WT) in the blocking buffer with a final amount of 50 ng biotinylated hACE2 (Sino Biological, cat# 10108-H08H-B) / 8 ng epitope 3 nanobody / 8 ng epitope 4 nanobody at each concentration and then incubated with the plate at room temperature for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... followed by rabbit monoclonal antibody against SARS-CoV-2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Microbiology 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibody against the SARS-CoV-2 nucleocapsid protein (1:1000, Sino Biologicals US Inc, Wayne, PA). After washing 3 times with wash buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated with a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked with 5% milk in PBST before the membrane was incubated with a primary antibody (rabbit anti-SARS-CoV-2 S2 polyclonal antibody, Sino Biological, Cat# 40590-T62, 1:2500 dilution) in PBST ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...