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Citations for Sino Biological :
751 - 800 of 831 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained in FACS buffer with anti-CEACAM6-FITC antibody (Sino Biological 10823-R408R, Sino Biological, Inc., Wayne, PA) at 10 μg/mL for 30 min at 4°C in the dark ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The cell culture supernatants were collected and analyzed by western blot analysis using a commercial antibody (Sino Biological Inc. Beijing, China) against SARS-CoV-2 RBD.
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bioRxiv - Microbiology 2023Quote: ... Antigen-positive cells were detected by indirect immunofluorescence assay (IFA) using an anti-HCoV-229E nucleoprotein antibody (Sino Biological, Beijing, China).
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bioRxiv - Microbiology 2023Quote: ... the SARS-CoV2 infected cells were fixed and immunostained with a SARS-CoV Nucleocapsid Antibody (Sino Biological, Catalogue number: 40143-MM05), followed by HRP-conjugated Goat anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Microbiology 2020Quote: ... DCs were pulsed with 20μg/mL SSP-1 peptide in the presence of 3μg/mL β-microglobulin (Sino Biological) at 37°C for 3 hours before use ...
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bioRxiv - Immunology 2020Quote: ... S protein was probed with anti-SARS-CoV-1 S1 subunit rabbit polyclonal antibody (40150-T62-COV2, Sino Biological); N protein was probed with anti-SARS-CoV1 NP rabbit polyclonal antibody (40413-T62 ...
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bioRxiv - Microbiology 2021Quote: ... Immunofluorescence staining was conducted using the SARS-CoV-2 nucleoprotein mouse monoclonal antibody (1:500; Sino Biological #40143-MM05), Alexa-Fluor Plus 488 anti-mouse antibody (1:1,000 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 Wuhan-Hu-1 Spike protein (NR-53524 BEI Resources) or MERS-CoV Spike protein (Sino Biological) was biotinylated with the Biotin-XX Microscale Protein Labeling Kit (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with an anti-SARS antibody (SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit monoclonal antibody; Sino Biological #40143-R001), diluted to 0.125 μg/mL in 3% BSA/PBS blocking solution for 1 h at room temperature ...
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bioRxiv - Immunology 2020Quote: Internalization of CCR7 was also examined with HEK293T cells stably expressing a mouse CCR7-GFP fusion construct (pCMV3-mCCR7-GFPSpark, Sino Biological Inc.). HEK293T cells were transfected with CCR7-GFP with Lipofectamin 2000 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1:1000 mouse antibody specific for SARS-CoV-2 nucleocapsid protein (Sino Biological, Beijing, China 40143-MM05) overnight at 4°C in blocking buffer consisting of 2% FBS and 2% BSA ...
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bioRxiv - Microbiology 2020Quote: ... were stained for 1 h with 0.01 mg/ml rabbit anti-SARS-CoV-2 Spike S1 subunit (clone#007, Sino Biologicals) and 0.03 mg/ml mouse anti-Tubulin IV (cloneONS.1A6 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 0777)/1 X DPBS % v/v solution (DPBST) and 50 µl/well of 0.05 µg/ml H3 (Sino Biological; 11231-RP02) or 0.5 µg/ml H3K4me3 (Millipore ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV were stained with mouse-anti-SARS-CoV nucleoprotein (40143-MM05, 1:400, Sino Biological) or rabbit-anti-SARS-CoV nucleoprotein (40143-T62 ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates were coated with Spike 1 protein of SARS-CoV-2 (0.1μg/100 μL, Sino Biological, 40591-V08H), the tested sera were diluted at 1:100 and added to each well ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with the SARS-CoV-2 S1 subunit (amino acids 1–685) (Sino Biological, China) at 1 μg/ml in PBS (50 ul/well) ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilized with 70% ethanol and stained with polyclonal rabbit anti-SARS-CoV nucleoprotein antibody (1:1000; 40588-T62, Sino Biological) followed by secondary Alexa488 conjugated goat-anti-rabbit antibody (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... DRG tissue lysates were immunoprecipitated over 2 hours at 4°C with protein A/G coupled with antibodies (rabbit anti-FcγRI 1:1000, Cat: 80016-R015, Sino Biological Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... a rabbit polyclonal antibody against the SARS-CoV-2 nucleocapsid protein (dilution: 1:4,000, catalog number: 40143-T62, Sino Biological) was applied to the slides for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
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bioRxiv - Pathology 2020Quote: ... HEK-Blue SR-AI and HEK-Blue CD36 cells were prepared from the parent cell line (HEK-Blue™ Null1-v) by stable transfection plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc.) using Hygromycin as the selective antibiotic ...
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bioRxiv - Molecular Biology 2021Quote: ... Imdevimab/REGN10987 and REGN10989 (Hansen et al., 2020) were cloned in pCMC3-untagged-NCV (SINO Biologics, Cat: CV011) and produced in 293T cells by SINO Biological (Beijing, China). The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Calculation of absolute copy number for TH and VGluT2 in each cell was determined with an external calibration curve based on a TH or VGluT2 plasmid cDNA (obtained, respectively, from Sino Biological (Beijing, China) and Harvard University (Boston ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 PSD and pCMV3 containing the SARS-CoV-2/COVID-19 Spike gene were co-transfected into 7 x 105 293F cells using Sinofection (Sino Biological, Beijing, China). The medium was replaced with fresh DMEM containing 10% FBS after overnight incubation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 3.7% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) and secondary antibody anti-Rabbit-IgG-FITC (Sigma ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... Immunohistochemical staining was performed with a monoclonal rabbit anti-SARS-CoV/SARS-CoV-2 nucleocapsid (NP) antibody (1:1000, 40143-R001, Sino Biological), followed by incubation with Dako EnVision+ System HRP ...
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bioRxiv - Immunology 2021Quote: ELISA plates were coated with RBD (SARS-CoV-2 WT, SARS-CoV-2 Omicron, SARS-CoV-1 RBD, Sino Biological Inc.) at 0.03 μg/mL and 1 μg/mL in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
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bioRxiv - Immunology 2022Quote: ... The Omicron BA.1 RBD and BA.2 RBD used in ELISA were purchased from Sino Biological (Cat. No. 40592-V08H121) and AcroBiosystems (Cat ...
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bioRxiv - Microbiology 2019Quote: ... and the A/H7 HA (EPI439507) and A/N9 NA (EPI439509) from A/Anhui/1/2013 (Anhui13) were purchased from Sino Biological. cDNAs were amplified by Platinum™ SuperFi™ PCR (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
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bioRxiv - Microbiology 2023Quote: ... using a combination of polyclonal antibodies against the viral spike protein (1:200 dilution of anti S1 RBD cat. no. 40592-T62, and 1:200 dilution of anti S2 cat. no. 40590-T62, both from Sino Biological), for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells were starved for 5 hours in DMEM/F12 supplemented with 1% PS and then stimulated with 100ng/mL BDNF (50240-MNAS, Sino Biological) for different time periods (5 ...
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bioRxiv - Immunology 2023Quote: ELISA assays were conducted by pre-coating ELISA plates with RBD (SARS-CoV-2 wild type, SARS-CoV-2 BA.1, SARS-CoV-2 BA.2 RBD, Sino Biological) at concentrations of 0.03 μg ml−1 and 1 μg ml−1 in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... IP complexes were sequentially eluted first with 50 ul of 1 mg/ml HA peptide (Sino Biological, catalog #PP100028, lot #IP15MA1401X) then boiling in sample buffer 95°C for 10 minutes.
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bioRxiv - Microbiology 2021Quote: 293F cells at the density of 6 × 105 cells/mL were seeded into 100 mL SMM 293-TII serum-free medium (Sino Biological, Cat. No. M293TII) one day before transfection ...
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bioRxiv - Immunology 2020Quote: ... and 7 days post transfection by gently pipetting cells into medium and labeled with an anti-RBD monoclonal antibody (clone D003 Sino Biological Catalog # 40150-D003) and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 antigen production in cells was detected by successive incubations with an anti-SARS-CoV nucleoprotein mouse monoclonal antibody (Sino Biological catalog# 40143-MM05), HRP IgG conjugate (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then fixed in 4% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171). Cells were then stained with anti-rabbit-IgG-FITC (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Viral infected cells were revealed by staining using primary monoclonal antibodies directed against SARS CoV-2 nucleocapsid (SARS CoV-2, Sino Biological Inc., 40143-R001) or primary monoclonal mouse antibodies directed against J2 (HCoV-229E ...