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Citations for Sino Biological :
451 - 500 of 831 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng Firefly-luciferase (FLuc) reporter constructs were mixed with storage buffer or storage buffer supplemented with recombinant 4E-BP1 (Sino Biological, 10022-H07E), eIF4A1wt or eIF4A1E183Q (eIF4A1DQAD ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Immunology 2021Quote: 96-well microtiter plates were coated over night at 4°C with 100ng per well of recombinant NTD (Sino Biological; Beijing, China #40591-V49H), SARS-CoV-2 Spike S2 or S1 Glycoprotein (Virion\Serion ...
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bioRxiv - Molecular Biology 2020Quote: ... 1A10 were prepared by immunizing BALB/c mice with recombinant SARS-CoV-2 RBD fused with a C-terminal mouse IgGFc tag (Sino Biological Inc, Beijing, China) using previously described protocols (Qu et al. ...
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Microbiology 2021Quote: The human ACE2 protein was purchased from Sino Biological (Beijing, China; Cat# 10108-H08H) and the human IgG1 Fc-tagged RBD proteins were made in-house using a method as previously described35 ...
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bioRxiv - Biochemistry 2021Quote: The cDNA for human ACE2 (Uniprot: Q9BYF1) was purchased from Sino Biological (HG10108-M). cDNAs encoding the SARS-CoV-2 genes for ORF6 ...
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bioRxiv - Biochemistry 2021Quote: ... Full-length human MAVS cDNA was transferred from pGEM- MAVS (Sino Biological HG15224-G) into the KpnI-NotI sites of the pCMV6-Entry vector (OriGene Technologies #PS100001 ...
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bioRxiv - Microbiology 2021Quote: The human ACE2 protein was purchased from Sino Biological (Beijing, China; Cat# 10108-H08H) and the human IgG1 Fc-tagged RBD proteins were made in-house using a method as previously described28 ...
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bioRxiv - Immunology 2020Quote: The MerTK (Mer cDNA ORF Clone, Human, untagged, pCMV3) was obtained from Sino Biological. Both pcDNAI-GAL4-CREB and 5xGAL4-TATA-luciferase were a gift from Richard Maurer(Sun et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The pCMV3-N-Myc-human AXL (HG10279-NM) plasmid was purchased from Sino Biological. The peGFP-C1-KRas4A ...
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bioRxiv - Biophysics 2021Quote: For affinity measurements of anti-human coronavirus spike glycoprotein HKU1 (40021-MM07-100, Sino Biological) to HCoV-HKU1 S1 protein ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligand blocking was performed by pre-adsorbing HPK with soluble human HER3 peptide (Sino Biological) at 10× molar excess in PBS before adding to cells ...
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bioRxiv - Immunology 2020Quote: ... Mouse-Human chimeric mAb D001 (SARS-CoV RBD mAb; Sino Biological Inc Cat# 40150-D001) was used as a control mAb ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed with PBS-T and further incubated with human ACE2-Fc (Sino Biological) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: The I-BAR domain of human Mtss1 was purchased from Sino Biological (Cat 13085-H10E). The human Plexin-D1 cytosolic domain was prepared ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with a rabbit (2019-nCoV) S1 antibody (1:50; Sino Biological, Duesseldorf, Germany) and then incubated for 1 hour at 37°C with secondary goat anti-rabbit antibodies conjugated with fluorescein isothiocyanate (FITC ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 µl [62.5 ng/ul] of human Argonaute 2 (hAgo2) protein (Sino Biologicals, North Wales, PA) and 15 µl of Assembling Buffer [2 mM Mg(OAc)2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were stained with primary antibodies Nucleocaspid protein SARS-CoV-2 (1:200, 40143-MM05, Sino Biological) and cardiac troponin T (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with anti-SARS-CoV-2 Spike protein antibodies (1:200, Sino Biological, 40150-R007) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Human polymeric immunoglobulin receptor (hpIgR) construct was assembled from a cDNA clone (Sino Biological, cat. HG10131-UT), which was used to extract the fragment encoding the hpIgR ectodomain ( GenBank:NP_002635 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed and stained with a polyclonal rabbit anti-SARS-CoV antibody (Sino Biological; 1:500). Staining was developed using a rabbit anti-SARS-CoV serum and a secondary alexa-fluor-labeled conjugate (Dako) ...
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bioRxiv - Microbiology 2023Quote: Cells were then incubated overnight at 4°C with rabbit anti-HCoV-229E nucleocapsid (1:400, Sino Biological) diluted in 1×SEA BLOCK ...
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bioRxiv - Cancer Biology 2019Quote: The vector encoding human Gal-14 gene (HG11371-CH) was obtained from Sino Biological (North Wales, PA, USA). The plasmid encoding human Gal-7 gene was obtained through the cloning of galectin-7 cDNA into sr⍺ eukaryotic expression vector using SpeI and BamHI restriction enzymes as previously described (Campion et al ...
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bioRxiv - Microbiology 2021Quote: ... we screened preconstructed human naïve phage scFv libraries with biotinylated SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells ...
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bioRxiv - Bioengineering 2023Quote: Full length cDNA of human CD123 (NM_002183.2) was obtained from a pCMV3 vector (Cat#HG10518-M, Sino Biological). The Hygromycin sequence was replaced by a Neomycin resistance cassette by Gibson Assembly ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Genetics 2020Quote: Mammalian cDNA ORF clones were used for AVP (human, HG17671-UT, NCBI Ref Seq: NM_000490.4, Sino Biological, Beijing, China) and MAOA (mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments encoding human hSSB1 and INTS3 were amplified by PCR from NABP2- GFPspark plasmid (Sino Biological, HG22790-ACG) and INTS3- GFPspark plasmid (Sino Biological ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated overnight with 100 ng/well (in 100 μL/well volume) of purified human CEACAM6 protein (Sino Biological). Standards containing known concentrations of the UCHT-2-03 recombinant protein or test samples were applied to the plate for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Biochemistry 2021Quote: Vero E6 cells were incubated with RBD protein (0.45 μg/mL, final concentration) followed by incubation with human anti-RBD antibody (primary antibody) (40150-D003, Sino Biological) and goat anti-human IgG AF488-conjugated antibody (secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: A Pro-CTSL clone was generated by sub-cloning the human Pre-Pro-CTSL ORF clone (in pMD18-T Simple Vector) obtained from Sino Biologicals Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA of human CD47 and mouse CD47 with an HA-tag at the C-terminal were purchased from Sino Biological and Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: The rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Cat# 40592-T62) or human SARS-CoV-2 S-NTD antibody (E-AB-V1030) was obtained from Sino Biological or Elabscience ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Evolutionary Biology 2023Quote: The plasmid containing the human SLC30A9 gene codifying for the ZnT9 transporter with an N-HA tag was obtained from Sino Biological Inc ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Cancer Biology 2021Quote: pCMV3-N-HA negative control vector and human RPRM plasmids (full length, S98A mutant and Δ(79-109) mutant) were purchased from Sino Biological Inc ...
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bioRxiv - Bioengineering 2020Quote: ... Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological ...
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bioRxiv - Cancer Biology 2020Quote: ... Prepared elastic Fab anti-Fc-functionalized PAA nanotextures were incubated with 20 μg/mL human ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (4°C ...
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bioRxiv - Biochemistry 2021Quote: Affinity-purified full-length EGFR was concentrated to 50 μM and treated with 100 μM human EGF (Novoprotein) or TGF-α (Sino Biological) for at least 5 mins on ice ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 1:500 dilution ratio of primary antibody specific for SARS-CoV nucleoprotein56 (Rabbit mAb) (Sino Biological Inc. China), which cross-reacting with the NP protein of SARS-CoV–2 as well ...
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bioRxiv - Microbiology 2021Quote: ... human (WT or H34Y) or mink ACE2 (WT or Y34H) were incubated with rabbit polyclonal antibody (Sino Biological Inc. China, Cat: 10108-T24) against ACE2 ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-Human Fc FAB patterns on PAA were incubated with 10 µg/mL ICAM1-hFc solution (Sino Biological, Cat# 10346-H03H-50) and α-collagen-1 Ab micropatterns on PAA gels were incubated with 1 mg/mL rat monomeric collagen-1 (Corning ...
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bioRxiv - Physiology 2022Quote: ... TIMP-1 (Sino Biological, Houston, TX; 1:2000), LOXL2 (Novus Biologicals ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were plated in 6-well plates (2×105 cells/well) and treated with 1 ng/mL LPS and 20 ng/mL mIFNγ (Sino Biological Inc., Beijing, China) for 24 h ...