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Citations for New England Biolabs :
301 - 350 of 1210 citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Methylation assays were performed using 100 ng of an unmethylated pUC19 plasmid purified from dam- /dcm- E.coli strain (C2925I, NEB) or a 1-kb fragment containing 14 CpG sites amplified from the Meis1 enhancer were used as DNA substrates for filter binding assays ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cloning was done in bacterial strain Escherichia coli NEB® 10-beta (New England Biolabs, Frankfurt a. M., Germany) grown at 37 °C in lysogeny broth [47] supplemented with 60 mg L-1 ampicillin (Applichem ...
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bioRxiv - Microbiology 2021Quote: ... difficile strain 630 genomic DNA and cloned into the modified pDIA6103 using NEBuilder Hifi DNA Assembly (New England Biolabs). The resulting pDIA6103 derivative plasmid was transformed into the E ...
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bioRxiv - Bioengineering 2021Quote: 2 μl of genomic DNA was used to amplify strain barcodes by PCR (Q5 NEB master mix, 22 cycles) using primers containing sequence-optimized spacers to maximize nucleotide diversity in Illumina sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Two separate 8-µl aliquots of beads derived from each strain were treated with 1 µl lambda phosphatase (NEB) in 10 µl reactions containing ...
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bioRxiv - Microbiology 2019Quote: ... Chromosomal deletion strains were constructed using the allele exchange vector pKAS32 digested with KpnI and SacI (NEB, High Fidelity). Luciferase reporters were constructed using the luciferase reporter vector pBBRlux digested with BamHI and SpeI (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... The transcription template for RNAI was produced by using p770 plasmid (from E. coli strain LK_2385) as template for PCR amplification using Q5 polymerase (NEB) and LK_3413 and LK_3414 primers to introduce a T7 class II promoter (Supplementary table 3) ...
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bioRxiv - Genomics 2020Quote: ... Transformation efficiencies of all strains were monitored and normalised using a stock solution of pLITMUS28 (New England Biolabs, USA). In addition ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were used for all subcloning steps and for permanent storage of transfected strains (New England Biolabs (NEB) - C2987I) ...
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bioRxiv - Biophysics 2022Quote: ... coli cells were used for all subcloning steps and for permanent storage of transfected strains (New England Biolabs (NEB) - C2987I) ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Molecular Biology 2024Quote: The strains used in this work were T7 express and shuffle T7 express Escherichia coli cells (New England Biolabs). All strains were grown in Luria-Bertani (LB ...
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bioRxiv - Cancer Biology 2021Quote: ... we used 50 μL Protein A or Protein G Magnetic beads (NEB) and washed twice with PBS with 5 mg/ml BSA and 4 μg of antibody coupled in 500 μl PBS with 5 mg/ml BSA overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Neuroscience 2021Quote: ... Lambda Protein Phosphatase (NEB) assay was performed as described by the supplier ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 protein (NEB M0641S) and phenol red injection dye ...
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bioRxiv - Developmental Biology 2021Quote: Cas9 protein (M0646, NEB) and sgRNAs against antisense of exons 14 ...
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting amplicons from each replicate/strain were cleaned up using the Monarch PCR & DNA Cleanup kit (New England Biolabs). Next ...
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bioRxiv - Synthetic Biology 2020Quote: ... marxianus strain CBS6556 (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) using Q5 High-Fidelity Polymerase (M4092L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Synthetic Biology 2020Quote: ... marxianus strain CBS6556 (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) using Q5 High-Fidelity Polymerase (M4092L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2021Quote: ... tuberculosis H37Rv (ATCC#25618) were procured through ATCC (USA). Escherichia coli (E. coli) strains DH5α and BL21 (DE3) were procured from NEB and were cultured in Luria–Bertani medium ...
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bioRxiv - Cell Biology 2020Quote: ... the TAP coding sequence was PCR-amplified from chromosomal DNA from a strain expressing Heh2-TAP (SBCPL42, Dharmacon yeast resources) using Phusion High fidelity DNA polymerase (New England BioLabs) and cloned into the PacI and AscI sites of pFA6a-his3MX6 and pFA6a-TRP1.
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bioRxiv - Cell Biology 2020Quote: ... or the same strain expressing OsTIR1 driven by the gra1-promoter with 50 μL of a PCR product using Q5 polymerase (NEB) with 500 bp homology arms flanking a tag (3xHA or AID-3xFLAG ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We amplified the wild-type EST1 gene from the genome of the haploid strain BY4742 by polymerase chain reaction (PCR) using the high-fidelity Q5 polymerase (NEB) and inserted it into the ΔEST1 cells used in [1] by CRISPR/Cas9 editing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Molecular Biology 2023Quote: ... these antibiotic resistant cassettes were excised by transforming the strains with a plasmid expressing the Cre recombinase (pDR244, BGSCID: ECE274) purified from RecA+ Escherichia coli (NEB) cells with the GeneJET Plasmid Miniprep Kit (Thermo) ...
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bioRxiv - Microbiology 2023Quote: ... and complement strains (wos2Δ::WOS2) were constructed using biolistic transformation of constructs amplified using double-joint PCR or Gibson Assembly (NEB), as previously described (primers ...
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bioRxiv - Microbiology 2023Quote: The RmlC expression constructs used in this study were constructed by PCR amplification of the rmlC alleles from strains MG1655 (rmlCWT) and BP27 (rmlCL122W) using the Q5 high-fidelity polymerase (NEB) and oligos listed in Table s1 and subsequent insertion into pBAD18-Chl via restriction digestion cloning.
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bioRxiv - Microbiology 2023Quote: ... open reading frame was amplified from genomic DNA of the 237 Moraxella bovoculi strain by PCR and cloned into the pTN7C130 vector using HiFi assembly (NEB). The pTN7C130 vector is a mini-Tn7 vector that integrates into the attTn7 site of P ...
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bioRxiv - Microbiology 2023Quote: Samples of a hundred micrograms of protein from the crude extracts of the strains of interest were incubated with Lambda phosphatase (λPP; 400 u per reaction; New England Biolabs) for 20 minutes at 30 °C [88] ...
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bioRxiv - Microbiology 2023Quote: ... the sequences of creS and their native promoter were amplified from strain CB15N and then assembled into the pCT133 or pCT155 backbone using Gibson assembly (New England Biolabs). For protein expression in E ...
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bioRxiv - Cell Biology 2023Quote: ... with s 3×HA sequence fused to the C-terminus and the ADH1 terminator sequence was amplified from genomic DNA of a yeast strain expressing Opi1-HA and subsequently cloned into the pRS416 vector using XhoI and SacII restriction enzymes (New England Biolabs). To overexpress INO1 and KCS1 ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally hexahistidine-tagged Sfr1 was co-expressed with Swi5 as an operon from a pET11b vector in an Escherichia coli BL21 DE3 strain (NEB) that had been pre-transformed with the pRARE2 plasmid (Novagen) ...
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bioRxiv - Microbiology 2023Quote: ... difficile VPI10463 and UK1 strains and purified PCR products were directly cloned into the pMSR0 vector using NEBuilder HiFi DNA Assembly (New England Biolabs). The pMSR0-derived plasmids containing the allele exchange cassettes of the target genes were transformed into E ...
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bioRxiv - Synthetic Biology 2024Quote: ... See Table S1 for a full list of derived yeast strains. NEB Turbo competent Escherichia coli (E. coli) from New England Biolabs (NEB was used for all DNA cloning and plasmid propagation work.
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bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
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bioRxiv - Developmental Biology 2024Quote: ... bulk-extracted genomic DNA from these strains was used to set up PCR reactions for each primer set using Quick Load Taq 2X Master Mix (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: Purified LPR1 protein was analyzed using Protein Deglycosylation Mix II (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Protein (200 µg) was precleared with protein G magnetic beads (New England Biolabs) and incubated overnight (4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved G protein was dephosphorylated by lambda protein phosphatase (NEB, Frankfurt, Germany), calf intestinal phosphatase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The protein ladder used was Colour Protein Standard Broad Range (NEB, Ipswich, US).
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bioRxiv - Developmental Biology 2020Quote: Alg2 cDNA was amplified with RT-PCR from the cDNA of wt Oryzias latipes Cab strain stage 18 embryos with Q5® High-Fidelity DNA Polymerase (New England Biolabs) by using primers with BamHI-HF (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... Successful generation of ΔpufX and ΔpufY strains was confirmed using PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, UK) and DNA sequencing (Eurofins).
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bioRxiv - Microbiology 2021Quote: ... Between 1-2kb of flanking regions upstream and downstream of the region of interest were amplified from parental strain using Q5 2x Master Mix (New England Biolabs, USA). The antibiotic resistance gene ermB was amplified from S ...
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bioRxiv - Microbiology 2022Quote: ... To gain fusion strain characteristic banding patterns the restriction digestion of genomic DNA was performed with PacI (New England Biolabs, USA). For PFGE analysis ...
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bioRxiv - Microbiology 2019Quote: ... Between 1-2kb of flanking regions upstream and downstream of the regions of interest were amplified from parental strains using Q5 2x Master Mix (New England Biolabs, USA). The antibiotic resistance gene ermB was amplified from S ...
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).