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Citations for New England Biolabs :
201 - 250 of 1210 citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... The virus lysate was then denatured by boiling in denaturing buffer (New England BioLabs) for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs ...
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Clones were selected and maintained in E.coli strain NEB5-alpha (NEB, C2987I). All constructs were verified by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2019Quote: ... Bacillus subtilis 3610 (33) and Escherichia coli strains NEB10β (New England Biolabs) and WM3064 were cultured aerobically on LB agar or in LB broth ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-SAG1-DHFR (AddGene #80322) ...
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bioRxiv - Microbiology 2019Quote: ... Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-BFD1-DHFR ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Bioengineering 2021Quote: Escherichia coli strains DH5α [15] and ER2523 (New England Biolabs, Ltd., UK) were grown in Luria Bertani (LB ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains Nico21 (DE3) pLysS or BL21-codon plus (New England BioLabs) were used for overexpression ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Virus stocks were generated by either transfecting purified in vitro transcribed viral RNA (HiScribe, NEB) or transfecting the infectious cDNA clone containing plasmid in BHK-T7 cells ...
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bioRxiv - Biochemistry 2023Quote: Infection in HCT116: Cells were infected with virus and 8 μg/mL polybrene (NEB, #H9268).
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... All cloning and strains were performed in dh10b genotype cells (NEB, Intact Genomics).
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bioRxiv - Bioengineering 2022Quote: Escherichia coli (E. coli) strain NEB 5-α (New England Biolabs, Hertfordshire, UK) was used for generation of genetic constructs ...
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bioRxiv - Microbiology 2020Quote: ... was used for strain D236 and the nicking enzyme combination Nt.BspQI/Nb.BbvCI (NEB) was used for strains D800 ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Bioengineering 2021Quote: This study used the Escherichia coli strains NEB10β (New England Biolabs, Ipswich, MA), Nissle 1917 as well as all strains listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains were performed using Monarch® Total RNA Miniprep Kit (NEB, UK) and SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Synthetic Biology 2023Quote: ... Discrete strains were assembled using the HiFi DNA Assembly kit (NEB, cat #E2621) from IDT gBlocks ...
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bioRxiv - Microbiology 2023Quote: ... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Synthetic Biology 2019Quote: The bacterial strains used in this study includes Escherichia coli DH5α (New England Biolabs), Escherichia coli Nissle 1917 (isolated from probiotic Mutaflor capsule) ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP2_KO_A and clpP2_KO_D primer pair (Table 3 ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP_tag fwd and rev primer pair (Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... coli JP313 ΔtolC strains using a Q5 high-fidelity DNA PCR (New England Biolabs). The primers used for these reactions were “EM029-TMKF1” (5’-ATGGCAAACTTTCTCGTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The Escherichia coli strain used for routine cloning was NEB 5α (New England Biolabs). The plasmids used in this article are listed below (Table S2) ...
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bioRxiv - Biophysics 2019Quote: ... This vector was transformed into BL21 (DE3) strain (New England BioLabs France, Evry, France). The transformed cells were inoculated into 1 liter of LB medium containing 50μg/ml kanamycin sulfate ...
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bioRxiv - Microbiology 2022Quote: ... coli host strain using the Monarch® Plasmid Miniprep Kit (NEB, Ipswich, MA, USA). The extracted plasmid was linearized with restriction enzyme BamHI (Promega ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from Ap strain HZ gDNA using Q5 DNA polymerase (New England Biolabs). Cloning into pET-28a and a modified pET41 was performed using restriction free PCR-based methods26 ...
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bioRxiv - Biochemistry 2024Quote: Plasmids required for generating mutant strains were prepared using HiFi DNA assembly (NEB, USA) and then transformed into S ...
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bioRxiv - Molecular Biology 2020Quote: The bacterial source strains used for this work were Escherichia coli NEB5α (New England BioLabs) for plasmid constructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs) 20 was used for cyclonal expression and library selections ...
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bioRxiv - Microbiology 2020Quote: The bacterial source strains used in this work were Escherichia coli NEB5α(New England Biolabs) for plasmid constructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... then transformed into the E.coli NEB Stable strain from New England Biolabs (NEB, MA, USA) for plasmid isolation ...
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bioRxiv - Cell Biology 2020Quote: ... These strains were transformed by electroporation [20] with the EcoNI-linearized (R0521S; New England Biolabs) pRK2 plasmid ...
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bioRxiv - Microbiology 2022Quote: ... coli strain used in this study for cloning purposes was NEB-5α (New England Biolabs) (Table 1) ...
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bioRxiv - Plant Biology 2023Quote: ... and affinity purified from Escherichia coli strain BL21 (DE3) using Amylose Resin (New England Biolabs) as previously described 5.