Labshake search
Citations for New England Biolabs :
251 - 300 of 1210 citations for Zika Virus NS1 Proteins Uganda Suriname Strains Duo Pack since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
-
bioRxiv - Bioengineering 2022Quote: ... coli NEB 10-β and BL21 (DE3) strain (both from New England BioLabs, Ipswich, USA) with pRI952 plasmid used for all fermentations ...
-
bioRxiv - Microbiology 2022Quote: ... Strain specific abundance was assessed with the Luna® Universal qPCR Master Mix (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... coli BL21 (DE3) strain and purified with amylose agarose beads (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... subtilis strain bearing Physp-bdbDC at the ycgO locus (bJZ185) using LongAmp Taq DNA Polymerase (NEB) and oligonucleotide primer pair (oJZ436 + oJZ437) ...
-
bioRxiv - Microbiology 2023Quote: ... aeruginosa strains using the primers indicated in Table S7 with Q5 DNA Polymerase (New England Biolabs), in reactions that added regions of homology to plasmid pUCP20 ...
-
bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
-
bioRxiv - Microbiology 2021Quote: ... in the 5’end of 30 mer RNA substrate we used the vaccinia virus capping enzyme following the manufacturer’s protocol (New England Biolabs), except that the methyl donor SAM was absent and 0.05 units of inorganic pyrophosphatase (New England Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2 -O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
-
bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
-
bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
-
bioRxiv - Molecular Biology 2019Quote: ... crassa strains expressing a Dam fusion was digested with 1 μL of DpnI (NEB, 20 units/μL); ligation to primer 5050 was carried out overnight at 16 °C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
-
bioRxiv - Biophysics 2020Quote: ... we used an alternative strategy that enables us to propagate expression plasmids in standard bacterial strains (NEB 5-alpha Competent E ...
-
bioRxiv - Biophysics 2020Quote: In vivo folding experiments were carried out in E.coli strain Lemo21(DE3) (New England Biolabs, NEB, C2528J). All plasmids used in this study are based on a backbone with a pUC origin ...
-
bioRxiv - Biophysics 2020Quote: In vivo folding experiments were carried out in E.coli strain Lemo21(DE3) (New England Biolabs, NEB, C2528J). All plasmids used in this study are based on a backbone with a pUC origin ...
-
bioRxiv - Microbiology 2022Quote: ... and dam−/dcm− (dam−/dcm− competent E. coli, #C2925) strains were purchased from NEB (Ipswich, MA, USA) and cultured in Luria-Bertani (LB ...
-
bioRxiv - Synthetic Biology 2021Quote: The CFP and GFP reporter plasmids were propagated in either dam-/dcm-strain C2925 (New England Biolabs) or dam+/dcm+ strain DH5-alpha Turbo (NEB C2984 ...
-
bioRxiv - Bioengineering 2023Quote: ... The sequence-confirmed plasmid was transformed into Escherichia coli strain BL21(DE3) chemically competent cells (NEB C2527) according to NEB’s transformation protocol and plated on LB plates containing 100 µg/ml carbenicillin.
-
bioRxiv - Molecular Biology 2023Quote: ... hassiacum strain DSM 44199 as a template for colony-PCR with Q5 High-Fidelity DNA polymerase (NEB) to generate a fragment cloned into initial vector pINIT (Cm25) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plasmids encoding HA-tagged LC3B proteins were generated by replacing the EGFP sequence in the EGFP plasmids with an HA-tag followed by a Tobacco etch virus protease sequence recognition site and a Flag-Tag (Genewiz) using AgeI and HindIII (New England Biolabs). Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs ...
-
bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA with retrotranscriptase from Moloney murine leukemia virus (Ref. M0253, New England BioLabs) and random hexanucleotides as primers by following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE, NEB) in the presence of GTP and S-adenosylmethionine (SAM) ...
-
bioRxiv - Biochemistry 2020Quote: ... a maltose binding protein (MBP) and a Tobacco Etch Virus (TEV) protease cleavage site in the N-terminal via Gibson assembly (New England Biolabs) as per the manufacturer’s protocol.
-
bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
-
bioRxiv - Microbiology 2021Quote: ... Virus titer was determined using SYBR-based one step qRT-PCR with Luna Universal One Step qRT-PCR reagent (NEB). QuantStudio 5 (Applied Biosystems ...
-
bioRxiv - Microbiology 2020Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
-
bioRxiv - Developmental Biology 2022Quote: ... using 200-300 ng of purified RNA as template and Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase (M0253, NEB). cDNA was synthesised using the standard first strand synthesis protocol with random hexamers (S1230 ...
-
bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
-
bioRxiv - Molecular Biology 2023Quote: ... A CRISPR array containing two repeats and one spacer targeting the gfp gene of recombinant Sindbis virus (SINV-GFP) was generated by annealing and extending two partially complementary DNA oligos with Q5 polymerase (NEB) (Table S1) ...
-
bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
-
bioRxiv - Microbiology 2024Quote: The ectodomains of the hemagglutinin proteins from selected influenza virus strains were ordered as synthetic DNA fragments from Twist Biosciences and cloned with a barcoded fragment encoding the last 46 amino acids of WSN HA as 3-segment assembly reaction into a construct containing the 3’ non-coding region of the packaging signal and signal peptide from A/WSN/1933 influenza HA and the a Read 1 Illumina sequence and the full 5’ packaging signal from A/WSN/1933 virus using Hifi Assembly Mastermix (NEB). The backbone for this cloning reaction was a pHH21 plasmid (16 ...
-
bioRxiv - Biochemistry 2023Quote: ... modified to include a tobacco etch virus (TEV) protease recognition sequence downstream of the 6xHis-tag47 using NEBuilder HiFi DNA assembly master mix (New England BioLabs). Sequences were verified by Sanger DNA sequencing [Genomics Shared Resource ...
-
bioRxiv - Microbiology 2024Quote: ... hepatitis delta virus (HDV) ribozyme and simian virus 40 (SV40) poly-A sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 60 min ...
-
bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
-
bioRxiv - Microbiology 2019Quote: ... Flanking regions were amplified from the parental strains using either Q5 polymerase or OneTag polymerase (New England Biolabs). The OE PTS-HAL was generated in the Δvp1 and wild-type backgrounds by replacing the native PTS operon promoter with an amiA promoter and kan gene ...
-
bioRxiv - Microbiology 2022Quote: ... 30 μg of total RNAs from USA300 and ST80 strains were reversed transcribed with AMV reverse transcriptase (NEB) using 5’ radiolabelled primers (for hlgC (PEC1 ...
-
bioRxiv - Molecular Biology 2022Quote: The recombinant pET6xHN-N constructs were transformed into Escherichia coli strain BL21(DE3) (New England Biolabs, MA, USA). The transformed clones were cultured at 37 °C in LB medium with 100 μg/mL Carbenicillin and were induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
-
bioRxiv - Biochemistry 2022Quote: Selenomethionine-derivatized FL-TgGAC was expressed in the methionine auxotroph T7 Express Crystal Escherichia coli strain (NEB #C3022) cultured in SelenoMethionine Medium Base plus Nutrient Mix (Molecular Dimensions MD12-501) ...
-
bioRxiv - Cell Biology 2023Quote: ... ERK7-BioID2-3xHA was created by transfecting the RHΔku80Δhxgprt strain (66) with 50 μL of a PCR product using Q5 polymerase (NEB) with 500 bp homology arms flanking a BioID2-3xHA tag together with 5 μg of a Cas9 plasmid that had been modified to express HXGPRT and also a gRNA targeting the C-terminus of ERK7 (37) ...
-
bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... MWCO 10,000 filters and then digested with 10 µl Tobacco etch virus nuclear-inclusion-a endopeptidase (TEV) (New England Biolabs, Ipswich, MA) overnight at 4°C ...
-
bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
-
bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...
-
bioRxiv - Microbiology 2022Quote: ... Protein samples were then mixed with Blue Protein Loading Dye (NEB) boiled for 10 minutes and separated on 10% SDS-PAGE gel for 50 minutes at 140 V ...
-
bioRxiv - Plant Biology 2021Quote: ... and extracted nuclear proteins were treated with lambda protein phosphatase (NEB) according to the manufacturer’s instruction.
-
bioRxiv - Genomics 2020Quote: ... Endogenous proteins were captured onto protein G-magnetic beads (NEB; #S1430S), washed extensively in IP buffer and used for POT1wtand POT1V29L source ...
-
bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
-
bioRxiv - Zoology 2023Quote: ... One μg of total RNA was reverse transcribed in a 20 μl reaction using Moloney murine leukemia virus reverse transcriptase (New England Biolabs, Evry, France) and random primers ...