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Citations for New England Biolabs :
401 - 450 of 548 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Turicibacter genes were amplified from template culture lysates with Phusion or Q5 DNA polymerase (NEB) and primers designed to amplify denoted Turicibacter genes ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were denatured by boiling with 3X SDS Blue Loading Buffer (New England Biolabs). Samples were resolved on SDS-PAGE gel and transferred onto nitrocellulose membranes (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The D614G S-protein was generated by introducing the mutation into the Wuhan reference strain via Q5 Site-directed mutagenesis (NEB). Other individual mutations were subsequently introduced into the D614G S by the same process ...
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting amplicons from each replicate/strain were cleaned up using the Monarch PCR & DNA Cleanup kit (New England Biolabs). Next ...
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bioRxiv - Synthetic Biology 2020Quote: ... marxianus strain CBS6556 (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) using Q5 High-Fidelity Polymerase (M4092L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Synthetic Biology 2020Quote: ... marxianus strain CBS6556 (Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands) using Q5 High-Fidelity Polymerase (M4092L, New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2021Quote: ... tuberculosis H37Rv (ATCC#25618) were procured through ATCC (USA). Escherichia coli (E. coli) strains DH5α and BL21 (DE3) were procured from NEB and were cultured in Luria–Bertani medium ...
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bioRxiv - Cell Biology 2020Quote: ... the TAP coding sequence was PCR-amplified from chromosomal DNA from a strain expressing Heh2-TAP (SBCPL42, Dharmacon yeast resources) using Phusion High fidelity DNA polymerase (New England BioLabs) and cloned into the PacI and AscI sites of pFA6a-his3MX6 and pFA6a-TRP1.
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bioRxiv - Cell Biology 2020Quote: ... or the same strain expressing OsTIR1 driven by the gra1-promoter with 50 μL of a PCR product using Q5 polymerase (NEB) with 500 bp homology arms flanking a tag (3xHA or AID-3xFLAG ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We amplified the wild-type EST1 gene from the genome of the haploid strain BY4742 by polymerase chain reaction (PCR) using the high-fidelity Q5 polymerase (NEB) and inserted it into the ΔEST1 cells used in [1] by CRISPR/Cas9 editing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Molecular Biology 2023Quote: ... these antibiotic resistant cassettes were excised by transforming the strains with a plasmid expressing the Cre recombinase (pDR244, BGSCID: ECE274) purified from RecA+ Escherichia coli (NEB) cells with the GeneJET Plasmid Miniprep Kit (Thermo) ...
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bioRxiv - Microbiology 2023Quote: ... and complement strains (wos2Δ::WOS2) were constructed using biolistic transformation of constructs amplified using double-joint PCR or Gibson Assembly (NEB), as previously described (primers ...
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bioRxiv - Microbiology 2023Quote: The RmlC expression constructs used in this study were constructed by PCR amplification of the rmlC alleles from strains MG1655 (rmlCWT) and BP27 (rmlCL122W) using the Q5 high-fidelity polymerase (NEB) and oligos listed in Table s1 and subsequent insertion into pBAD18-Chl via restriction digestion cloning.
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bioRxiv - Microbiology 2023Quote: ... open reading frame was amplified from genomic DNA of the 237 Moraxella bovoculi strain by PCR and cloned into the pTN7C130 vector using HiFi assembly (NEB). The pTN7C130 vector is a mini-Tn7 vector that integrates into the attTn7 site of P ...
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bioRxiv - Microbiology 2023Quote: Samples of a hundred micrograms of protein from the crude extracts of the strains of interest were incubated with Lambda phosphatase (λPP; 400 u per reaction; New England Biolabs) for 20 minutes at 30 °C [88] ...
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bioRxiv - Microbiology 2023Quote: ... the sequences of creS and their native promoter were amplified from strain CB15N and then assembled into the pCT133 or pCT155 backbone using Gibson assembly (New England Biolabs). For protein expression in E ...
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bioRxiv - Cell Biology 2023Quote: ... with s 3×HA sequence fused to the C-terminus and the ADH1 terminator sequence was amplified from genomic DNA of a yeast strain expressing Opi1-HA and subsequently cloned into the pRS416 vector using XhoI and SacII restriction enzymes (New England Biolabs). To overexpress INO1 and KCS1 ...
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bioRxiv - Biochemistry 2023Quote: ... N-terminally hexahistidine-tagged Sfr1 was co-expressed with Swi5 as an operon from a pET11b vector in an Escherichia coli BL21 DE3 strain (NEB) that had been pre-transformed with the pRARE2 plasmid (Novagen) ...
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bioRxiv - Microbiology 2023Quote: ... difficile VPI10463 and UK1 strains and purified PCR products were directly cloned into the pMSR0 vector using NEBuilder HiFi DNA Assembly (New England Biolabs). The pMSR0-derived plasmids containing the allele exchange cassettes of the target genes were transformed into E ...
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bioRxiv - Synthetic Biology 2024Quote: ... See Table S1 for a full list of derived yeast strains. NEB Turbo competent Escherichia coli (E. coli) from New England Biolabs (NEB was used for all DNA cloning and plasmid propagation work.
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bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
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bioRxiv - Developmental Biology 2024Quote: ... bulk-extracted genomic DNA from these strains was used to set up PCR reactions for each primer set using Quick Load Taq 2X Master Mix (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... 14-17 A260 of lysate was digested with 800 U/A260 of micrococcal nuclease (MNase, NEB) at 25°C with shaking at 14,500 rpm for 20 min in lysis buffer supplemented with 2 mM CaCl2 and 500 U RNase Inhibitor ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was then centrifuged and the supernatant was passed over an amylose column (New England Biolabs) and washed for 4 hours ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the lysate was pre-cleared as follows: 100 μl of streptavidin magnetic beads (New England Biolabs) were washed in 1 ml of 1×PBS for 3 times and the FLAG-tagged Hyl1La lysate was added to the washed beads ...
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bioRxiv - Developmental Biology 2022Quote: ... Ribosome footprints were generated by incubating the lysate with 3 U/µg of micrococcal nuclease (NEB) for 40 min at 25° C ...
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bioRxiv - Developmental Biology 2019Quote: ... Crude cell lysate was passed through a Sepharose column containing 5mL amylose resin (New England Biolabs) and the MBP-tagged GFP-NT-Lysenin fusion protein was eluted with fresh 10 mM maltose in 1X PBS (pH 7.4) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the lysate was pre-cleared as following: 100 μl of streptavidin magnetic beads (New England Biolabs) were washed in 1 ml of 1×PBS for 3 times and the FLAG-tagged TBP::NveRLR/wild-type lysate was added to the washed beads ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was then incubated with 200 μL of oligo-dT 25 Magnetic Beads (NEB, S1419S) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Genomics 2022Quote: ... before dilution of the lysate in 10ml of 1x T4 DNA ligase buffer (New England Biolabs) plus 1% Triton-X100 and incubation for 1 hr at 37°C ...
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bioRxiv - Genomics 2022Quote: ... Tn5 transposase was bound by mixing the cleared lysate with 30 mL of chitin resin (NEB) in a column and rotating end-to-end for 30 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Clarified lysates (approximately 15 A260 units) were digested with 800 U/A260 MNase (New England Biolabs) for 1.5 h (25°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 µg of the lysate was digested with 1000 U micrococcal nuclease (NEB, Cat. # M0247S) for 30 min at 22°C ...
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bioRxiv - Genomics 2023Quote: RNA was extracted from the cell lysates using the Monarch Total RNA Miniprep kit (NEB T2010) and its quality was spot-checked for random samples using the Bioanalyzer High Sensitivity RNA Kit (Agilent 5067-1513) ...
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bioRxiv - Developmental Biology 2020Quote: Alg2 cDNA was amplified with RT-PCR from the cDNA of wt Oryzias latipes Cab strain stage 18 embryos with Q5® High-Fidelity DNA Polymerase (New England Biolabs) by using primers with BamHI-HF (New England Biolabs ...
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bioRxiv - Biochemistry 2021Quote: ... Successful generation of ΔpufX and ΔpufY strains was confirmed using PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, UK) and DNA sequencing (Eurofins).
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bioRxiv - Microbiology 2021Quote: ... Between 1-2kb of flanking regions upstream and downstream of the region of interest were amplified from parental strain using Q5 2x Master Mix (New England Biolabs, USA). The antibiotic resistance gene ermB was amplified from S ...
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bioRxiv - Microbiology 2022Quote: ... To gain fusion strain characteristic banding patterns the restriction digestion of genomic DNA was performed with PacI (New England Biolabs, USA). For PFGE analysis ...
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bioRxiv - Microbiology 2019Quote: ... Between 1-2kb of flanking regions upstream and downstream of the regions of interest were amplified from parental strains using Q5 2x Master Mix (New England Biolabs, USA). The antibiotic resistance gene ermB was amplified from S ...
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Microbiology 2022Quote: ... the corresponding ORFs were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and SphI enzymes (New England Biolabs, USA) (ECP_3022 ...