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Citations for New England Biolabs :
201 - 250 of 548 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The mRNAs were capped using the vaccinia virus capping enzyme kit (New England Biolabs) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Clones were selected and maintained in E.coli strain NEB5-alpha (NEB, C2987I). All constructs were verified by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2019Quote: ... Bacillus subtilis 3610 (33) and Escherichia coli strains NEB10β (New England Biolabs) and WM3064 were cultured aerobically on LB agar or in LB broth ...
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bioRxiv - Genetics 2019Quote: ... coli strains NEB10®-beta (New England BioLabs Inc., Ipswich, MA, USA), and XL1-blue (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-SAG1-DHFR (AddGene #80322) ...
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bioRxiv - Microbiology 2019Quote: ... Bradyzoite reporter strain parasites were transfected with 50 μg of AseI (NEB) linearized pU6-BFD1-DHFR ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Bioengineering 2021Quote: Escherichia coli strains DH5α [15] and ER2523 (New England Biolabs, Ltd., UK) were grown in Luria Bertani (LB ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain DH10-β cells (45 μL each; C3019I, New England Biolabs) thawed on ice for 10 minutes and mixed gently by tapping ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli strain K12 (NEB Turbo Competent E. coli, New England Biolabs #C2984) was used for routine cloning ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid was transformed into Escherichia coli expression strain BL2lλDE3 (New England Biolabs) for expression ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Microbiology 2023Quote: The strain Escherichia coli NEB 5-alpha (New England Biolabs, NEB C2987H) was used as a cloning strain ...
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bioRxiv - Biochemistry 2023Quote: ... coli strains Nico21 (DE3) pLysS or BL21-codon plus (New England BioLabs) were used for overexpression ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Virus-encoding plasmids were generated using Gibson Assembly (NEB Gibson Assembly kit, NEB, Ipswich, MA). Insert amplicons containing 25 bp overlaps to target regions were generated using Kapa HiFi Hotstart™ high-fidelity polymerase (Kapa Biosystems/Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Virus stocks were generated by either transfecting purified in vitro transcribed viral RNA (HiScribe, NEB) or transfecting the infectious cDNA clone containing plasmid in BHK-T7 cells ...
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bioRxiv - Biochemistry 2023Quote: Infection in HCT116: Cells were infected with virus and 8 μg/mL polybrene (NEB, #H9268).
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed with Moloney murine leukemia virus (M-MuLV) transcriptase (New England Biolabs). The purity and concentration of RNA were measured using NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... lysates were additionally dephosphorylated using Lambda protein phosphatase (NEB) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Protein lysates were generated using cell lysis buffer (NEB) and brief sonication on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then applied to amylose resins (NEB, E8021) and washed with 20 column volumes of binding buffer supplemented with 14 mM beta-mercaptoethanol ...
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bioRxiv - Genetics 2019Quote: ... the nuclear lysates were digested with 800U KpnI (NEB) overnight at 37°C and 950 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... all Orai1 lysate preparations were treated with PNGase (NEB) to remove all glycosylation ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with amylose resin (New England Biolabs) for 2 hours ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli K-12 strain NEB DH-10 Beta (New England Biolabs, MA, USA) unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... All cloning and strains were performed in dh10b genotype cells (NEB, Intact Genomics).
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bioRxiv - Bioengineering 2022Quote: Escherichia coli (E. coli) strain NEB 5-α (New England Biolabs, Hertfordshire, UK) was used for generation of genetic constructs ...
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bioRxiv - Microbiology 2020Quote: ... was used for strain D236 and the nicking enzyme combination Nt.BspQI/Nb.BbvCI (NEB) was used for strains D800 ...
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bioRxiv - Immunology 2020Quote: ... CD45 catalytic domain DNA constructs were transformed into the BL21 (DE3) strain (NEB) Escherichia coli and plated on LB agar with ampicillin (100 mg/ml) ...
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bioRxiv - Bioengineering 2021Quote: This study used the Escherichia coli strains NEB10β (New England Biolabs, Ipswich, MA), Nissle 1917 as well as all strains listed in Table 1 ...
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bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
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bioRxiv - Microbiology 2023Quote: ... aureus strains were performed using Monarch® Total RNA Miniprep Kit (NEB, UK) and SensiFAST cDNA Synthesis Kit (Bioline ...
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bioRxiv - Synthetic Biology 2023Quote: ... Discrete strains were assembled using the HiFi DNA Assembly kit (NEB, cat #E2621) from IDT gBlocks ...
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bioRxiv - Developmental Biology 2023Quote: ... Freshly laid wild-type TU-strain embryos were injected with SpCas9 protein (NEB) mixed with gRNAs (~300 ng/ul) ...
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bioRxiv - Molecular Biology 2023Quote: ... the enzymatic activity of the commerically availablale recombinant vaccinia virus methyltransferase mRNA 2’O-methyltransferase (NEB), abbreviated hereagter as VMTR1 ...
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bioRxiv - Biochemistry 2019Quote: ... the cleared lysate was loaded onto a amylose resin (NEB) and eluted with a maltose gradient ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein/lysate sample de-N-glycosylation using PNGase F (NEB), Endo S (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were flowed over 2 mL amylose resin (NEB) equilibrated with MBP lysis buffer to capture MBP-tagged protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The clarified lysate was added to equilibrated Amylose resin (NEB Cat no ...
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bioRxiv - Microbiology 2019Quote: ... Clarified lysate was added to a chitin resin column (NEB) and washed with Buffer A ...
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bioRxiv - Immunology 2020Quote: ... lysates were digested with endoglycosidase H (endoH) or PNGaseF (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysates were treated with Lambda phosphatase (New England BioLabs). For Western analysis ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was incubated with amylose resin (New England Biolabs), and MBP-Ola1p or MBP-Ola1p-monoGFP was eluted in elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were incubated with preequilibrated amylose beads (New England BioLabs) for 1 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Clarified lysates were loaded onto NEBExpress Ni spin columns (NEB), washed once with lysis buffer containing 10 mM imidazole ...
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bioRxiv - Cancer Biology 2023Quote: ... lysates were subject to PNGase F treatment (New England Biolabs) to deglycosylate the transporter proteins ...