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Citations for New England Biolabs :
451 - 500 of 548 citations for Zika Virus Lysate MR766 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... PCR assays were performed with the strain-specific multiplex primers from (46) and Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) in 20 µl reactions ...
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bioRxiv - Plant Biology 2023Quote: ... Strep–PIF4 recombined proteins were expressed in the Escherichia coli BL21 (DE3) strain and then purified using amylose resin (NEB, E8021S). DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... A total of 4.8 × 109 cells were harvested and total RNA from three biological replicates was extracted for each strain using the Monarch® Total RNA Miniprep (NEB) Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μg of protein lysates of each condition was treated with either Endo H (New England Biolabs) or PNGase F for 1 h at 37º C ...
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bioRxiv - Bioengineering 2019Quote: ... the lysate was digested by ∼0.2 mg/ml Proteinase K (New England Biolabs, Cat. # P8102 or P8107S) at 55°C for ∼1 day ...
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bioRxiv - Biochemistry 2021Quote: 20 µg protein lysate was incubated with lambda phosphatase according to the manufacturer’s protocol (New England Biolabs). Afterwards ...
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bioRxiv - Plant Biology 2022Quote: ... The lysate (input) was then incubated with 200 μl oligo(dT)25 magnetic beads (New England Biolabs) for 1 h at 4°C on a rotator ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: Env solubilized from cell lysates and VLPs was denatured by boiling in denaturing buffer (New England Biolabs) for 10 minutes ...
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bioRxiv - Genetics 2021Quote: ... Genotyping was carried out by conventional PCR using crude DNA lysates and Taq polymerase (New England Biolabs). PCR products destined for cloning were made with Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75-thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Cancer Biology 2021Quote: ... Bacterial lysates were prepared and the fusion protein purified over a chitin binding column (NEB labs, UK). HA-Ub75- thioester was obtained by incubating the column material with mercaptosulfonate sodium salt (MESNa ...
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bioRxiv - Genomics 2021Quote: ... first-level PCR reactions were performed using 1μl PCR-compatible lysate as a template for a 6.25μl Q5 (NEB) PCR reaction according to the manufacturer’s protocol (annealing temperature ...
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bioRxiv - Biophysics 2023Quote: ... The clarified and filtered lysate was loaded on 1 mL of IgG Sepharose fast flow resin (NEB). The column was washed with lysis buffer and TEV cleavage buffer (lysis buffer with 75 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: Cellular RNA was extracted from cell lysates using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The lysates (50 μl) were then incubated at 55°C with 1.25 μl proteinase K (NEB, P8107S). After 1h of incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 1 μl of Deglycosylation mix II (NEB, cat# P6044S) or distilled water ...
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bioRxiv - Immunology 2023Quote: Cleared cell lysates were incubated with PNGase F (500–1,000 U per 20 μl of lysats, NEB) for 30–45 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cell lysates using the Monarch Total RNA Miniprep Kit (T2010S, New England BioLabs) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared bacterial lysate from 1L of culture was incubated with 2mL of equilibrated amylose resin (NEB #E8021L) in a rotator overnight at 4C ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Genetics 2021Quote: ... or the ura3(R235A) strain (H06701) was isolated as described previously and used as template in a Phusion (New England Biolabs, Ipswich, MA) or Taq DNA Polymerase ((New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... Confirmation by Southern blot was accomplished by digesting the genomic DNA of the parental and putative deletion strains with AgeI (NEB, Ipswich, MA), separating the DNA fragments by gel electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: DNA encoding XFP1-10 protein was transformed and expressed under the control of an arabinose inducible promoter in Escherichia coli strain BL21(DE3) (New England BioLabs, Ipswich, MA). Cells were grown in Luria broth medium to an initial optical density of 0.2 ...
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bioRxiv - Genetics 2020Quote: Genomic DNA from wildtype animals (N2) and from strains bearing the hcp-6(mr17) mutation was amplified using a high-fidelity polymerase (Phusion®, NEB #M0530S) and the following primer pairs:
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bioRxiv - Molecular Biology 2022Quote: ... Strains with chromosomal insertions were constructed by amplifying overexpression cassettes from previously constructed plasmids with Phusion polymerase (New England Biolabs, Evry, France) and oligonucleotides eo-chrXI-f and eo-chrXI-r (Supplementary materials) ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... Diluted lysate was then incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
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bioRxiv - Microbiology 2022Quote: Cell lysates were prepared as described above used as DNA template for PCR amplification using Phusion polymerase (NEB) and oligonucleotides that anneal 150 bp outside of wzc ...
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bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... A 10% fraction of the chromatin lysate was digested using 200 U of Taq I (New England Biolabs) at 60°C for 7 h ...
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bioRxiv - Biochemistry 2021Quote: ... The second fraction of the clarified lysate was loaded onto 200 μl of Amylose Resin (New England Biolabs) equilibrated in Amy-Strep wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... DNA lysate was used as genotyping PCR template with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and enhancer specific genotyping primer pair spanning the upstream and downstream Cas9-guide RNA cleavage sites (Table S19) ...
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bioRxiv - Microbiology 2022Quote: For cloning/gene manipulation and DNA transfer into Methanothermobacter thermautotrophicus ΔH we utilized the Escherichia coli strains NEB stable (New England Biolabs, Frankfurt/Main, Germany) and S17-1 24 ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
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bioRxiv - Cell Biology 2021Quote: Whole cell lysates or eluted proteins after affinity purification were treated with Protein Deglycosylation Mix II (NEB, Cat# P6044), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was incubated with 150 μl of oligo (dT)25 cellulose beads (New England BioLabs, cat no. S1408S) for 15 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Then homogenized lysate incubated with 0.80 mL of pre- equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) for 1 h at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cell lysates from FLAG-ACBD4(wACBD5_FFAT)/5 (mFFAT) expressing cells were treated for 1 h with λPP (New England BioLabs) as described above ...
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bioRxiv - Cancer Biology 2019Quote: ... 25ug of each protein lysate were diluted into 3xSDS gel loading buffer (New England Biolabs Cat# B7703S, Lpswich, MA) and heated to 95°C for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and the remaining cytoplasmic lysate was supplemented with 0.4M CaCl2 and 4000 gel units micrococcal nuclease (New England Biolabs), and incubated at 37°C (30 min ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins isolated from cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure 15 ...
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bioRxiv - Cell Biology 2023Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... asynchronous or mitotic HeLa cell lysates were incubated with 0.9 mM MnCl2 and 7 U/µl λPP (New England Biolabs) or 0.9 mM MnCl2 and water for 1 h at 30 °C.