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Citations for New England Biolabs :
101 - 150 of 5851 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Equal amounts of RNA (~50 ng) were used for preparation of cDNA libraries with the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) with some minor changes to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Amplified cDNA was sheared to target 300bp fragment size and libraries were prepared using NEBNext DNA Library Prep (New England BioLabs). 50bp Paired End (PE ...
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bioRxiv - Neuroscience 2022Quote: ... After depletion 80 ng of each RNA sample was used to synthesized cDNA and make a library using the NEBNext Ultra Directional RNA library kit for Illumina (NEB).
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bioRxiv - Genomics 2022Quote: Illumina libraries were constructed from 100 ng of amplified cDNA libraries using the NEBNext Ultra II FS library prep kit for Illumina (New England Biolabs) with some modifications ...
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bioRxiv - Physiology 2022Quote: ... Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The 30 fish were sequenced for 100 base pair reads on one lane of a NovaSeq 6000 (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cDNA library was constructed following the manufacturer’s instructions of NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... Isolated mRNA was used to generate dual-indexed cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Eight cycles of PCR amplification were applied to all the libraries ...
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bioRxiv - Developmental Biology 2022Quote: ... A total amount of 1 μg of RNA per sample was used to prepare cDNA libraries generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: ... and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB). Libraries were sequenced on Illumina NovaSeq platforms as paired-end through Novogene mRNA sequencing services ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified fragmented mRNA was then used to generate cDNA libraries for sequencing using NEBNext Ultra Directional RNA library Kit (E7420S, NEB) following the protocol provided by the manufacturer with these modifications ...
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bioRxiv - Immunology 2021Quote: ... cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L). Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (human ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the enriched mRNA used to generate cDNA libraries with unique barcodes (Next UltraII Directional RNA Library Prep kit for Illumina, New England Biolabs). The cDNA libraries were pooled and subjected to RNA-Seq analysis at the UF-ICBR using the Illumina NextSeq 500 platform ...
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bioRxiv - Plant Biology 2022Quote: ... and the cDNA library was constructed with the NEBNext Ultra RNA Library Prep Kilt for Illumina (NEB, Ipswich, MA, USA), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA samples were directly proceeded for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following the library preparation protocol ...
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bioRxiv - Zoology 2022Quote: ... Stranded cDNA libraries were created with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Fish were sequenced for 100 base pair reads on one lane of a NovaSeq 6000 (Illumina) ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L). Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (human ...
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bioRxiv - Zoology 2023Quote: ... followed by the production of stranded cDNA libraries with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Individual barcodes were applied with NEBNext dual adaptors (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... the cDNA libraries were prepared from prepared RNA by Novogene using a Next® UltraTM RNA Library Preparation Kit (NEB) and sequenced using a NovaSeq 6000 platform (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... and processed into a cDNA library for Illumina with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Dual-index primers were conjugated with NEBNext Multiplex Oligos for Illumina (Set1 ...
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bioRxiv - Microbiology 2023Quote: ... cDNA libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit with rRNA Depletion (New England Biolabs) and sequenced using an Illumina NovaSeq 6000 with 150×150 cycle paired end sequence run ...
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bioRxiv - Cell Biology 2023Quote: ... RNA of the best quality from three replicates of each experiment were used for preparation of cDNA libraries for NGS using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... We converted oligo(dT)-selected RNA into a cDNA library for mRNA sequencing using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (NEB) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... A 250-300 bp insert cDNA library was generated by using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S). Transcriptome sequencing was performed on an Illumina NovaSeq 6000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA recovered from the IP was used for cDNA library preparation using the NEB Next Small RNA Library Prep Set for Illumina (NEB, E7330S) according to the manufacturer instructions.
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bioRxiv - Cancer Biology 2019Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext Ultra DNA Library Prep Kit (NEB, E7645). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA, USA inc.) for Illumina following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: RNA recovered from the PA-ac4C-seq and PAR-CLIP procedures were used for cDNA library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S), then sequenced using Illumina NextSeq 500 ...
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bioRxiv - Developmental Biology 2022Quote: ... Briefly 500pg of ribozero treated RNA was fragmented and used to produce cDNA libraries using the NEBnext Ultra RNA library prep kit (NEB, E7530S) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... following the manufacturer’s instructions and the recovered poly(A) selected RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The final RNA concentration was determined using Qubit RNA high Sensitivity Assay Kit and 100ng of RIP-purified RNA was used for cDNA library preparation using NEBNext Ultra II directional RNA library kit (NEB, E7760) following library preparation protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’adapter (for C2C12 libraries) or dU adapter (for HEK293T libraries) was ligated to the cDNA with Quick T4 DNA ligase (NEB, M2200L) and incubated in 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... and PCR amplification to prepare cDNA libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, USA). Library preparation and next-generation sequencing were outsourced to Rhelixa Inc ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol and cDNA library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Massachusetts, USA). DNA library quality control was done by measuring concentration using the Qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Two micrograms of total RNA were used for first-strand cDNA synthesis using AMV reverse transcriptase (New England Biolabs). A quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Genomics 2021Quote: ... and converted to cDNA using Ultra II Directional RNA Library Prep Kit (NEB E7760).
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis and amplification were performed with Next Ultra RNA Library Preparation Kit (NEB). Libraries were sequenced on a HiSeq3000 (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... and used for cDNA library generation with the NEBNext Ultra kit (New England BioLabs), without enrichment ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified using NEBNext Multiplex Oligos for Illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified using NEBNext Multiplex Oligos for illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... followed by the second strand cDNA synthesis using dTTP for non-directional library (NEB Next® Ultra RNA Library Prep Kit for Illumina®) ...
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bioRxiv - Developmental Biology 2023Quote: The TrackerSeq lineage libraries retrieved from cDNA were amplified with Q5 polymerase (NEB, M094S) in a 50-µl reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... and the cDNA libraries were purified using the Monarch PCR & DNA Cleanup Kit (NEB). High-throughput sequencing was performed on an Illumina NextSeq 550 in paired-end mode with 150 cycles per end.
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bioRxiv - Genomics 2022Quote: The library for high-throughput sequencing was prepared in a two-step PCR (Q5 high-fidelity DNA polymerase, NEB). In PCR1 ...
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bioRxiv - Genomics 2020Quote: The library was prepared for high-throughput sequencing in two rounds of PCR (Q5 High-Fidelity DNA Polymerase, NEB). In PCR1 ...
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bioRxiv - Molecular Biology 2022Quote: Sequencing libraries were prepared from 52.5 μg gDNA using two-step PCR strategy with NEBNext Ultra II Q5 Polymerase (NEB). First the gRNA regions were enriched from the genome ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
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bioRxiv - Biochemistry 2024Quote: Sequencing libraries for each sorted bin were generated by two-step PCR using a Phusion® DNA Polymerase (NEB) kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Isolated mRNAs were reverse-transcribed into double stranded cDNA and subjected to sequencing library construction using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: A total of 1 μg of RNA was used for cDNA library construction at Novogene using an NEBNext Ultra 2 RNA Library Prep Kit for Illumina (New England Biolabs; Cat.# E7775) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Complementary DNA (cDNA) libraries were constructed with the Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). cDNA concentrations were measured using a Qubit fluorometer with a dsDNA BR/HS kit (Invitrogen ...