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Citations for New England Biolabs :
301 - 350 of 5851 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... Selectively captured polyadenylated RNAs (1μg) were ligated directly to an DNA/RNA hybrid adapter (5’-CTACAC GACGCTrCrUrUrCrCrGrArUrCrUrNrNrN-3’) using T4 RNA ligase (NEB) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: For DNA-RNA hybrid IP (DRIP) non-crosslinked lysates were incubated (1 h, 37°C) with 10U RNaseH (NEB) prior to immunoselection ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next generation sequencing (Illumina HiSeq 2500 ...
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bioRxiv - Molecular Biology 2022Quote: ... libraries were generated using the Ultra II library preparation kit (NEB), then sequenced by paired-end sequencing with a 75 cycle high-output Nextseq 500 kit (Illumina).
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared with NEBNext Directional RNA Library Prep Kit (BioLabs). Pooled libraries (Table S1 ...
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bioRxiv - Molecular Biology 2019Quote: ... Libraries were quantified using qPCR (NEBNext Library Quantification, New England Biolabs) and an Agilent Bioanalyzer and then pooled for sequencing on an Illumina NextSeq 500 ...
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bioRxiv - Neuroscience 2019Quote: ... library preparation (NEBNext Ultra DNA Library Prep Kit for Illumina, NEB), and sequencing (Illumina Hiseq2500/4000 platform) ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were then quantified (NEBNext Library Quantification Kit; New England Biolabs) and sequenced with a 600 cycle MiSeq Reagent Kit (251×151 ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were quantified using the NEBNext Library Quant Kit (NEB, E7630S), then pooled at same molarity and paired-end sequenced with a P2-100 kit from Illumina in a NextSeq2000 sequencer following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation and multiplexing for ChIP- seq libraries were performed using the NEBNext ChIP-seq library Prep kit (NEB). The sequencing of ChIP-seq and input control libraries was performed using Illumina HiSeq-2500.
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... These two oligos were ligated by incubation with T4 ligase (NEB; 1U/μl T4 DNA ligase and 1× T4 DNA ligation buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Two different concentrations (80 and 160 Gel Units) of MNase (NEB) were used ...
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bioRxiv - Microbiology 2021Quote: ... the two fragments were generated using Phusion PCR (New England Biolabs) by amplifying the TurboGFP open reading frame from the vector pGIPZ (Thermo Scientific Open Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... We used two high fidelity PCR systems—Q5 (NEB cat. M0492), and Platinum SuperFi II (Invitrogen cat ...
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bioRxiv - Microbiology 2022Quote: pJJW101 was constructed using two stepwise NEBuilder HiFi assemblies (NEB, E5520S) followed by restriction and ligation ...
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Circularization of rv0678 for genotypic bedaquiline resistance testing of Mycobacterium tuberculosisbioRxiv - Molecular Biology 2022Quote: Two micrograms of amplicon was digested with Lambda Exonuclease (NEB, USA) in a 30ul reaction at 37°C for 30 minutes according to the manufacturer’s instructions ...
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FACS-Sortable Picoreactors for Ultra High-throughput Screening of Catalysts in Biphasic EnvironmentsbioRxiv - Bioengineering 2024Quote: ... the two inner solutions were comprised of 1) PURExpress reagents (NEB) or 2 ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized using the ProtoScriptII First Strand cDNA synthesis kit (NEB E6560S). Cloning of the full length admats9 from pooled cDNA from 3 ...
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bioRxiv - Microbiology 2019Quote: ... cDNA synthesis was performed using Protoscript II First Strand cDNA Synthesis kit (NEB) using random primers ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was prepared with AMV First Strand cDNA kit (New England Biolabs) using oligo-dT to prime the reaction ...
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bioRxiv - Immunology 2022Quote: ... and converted to cDNA with First Strand cDNA Synthesis Kit (E6300, NEB, USA). From the cDNA ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized using First-strand cDNA synthesis kit (New England BioLabs, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA synthesis with ProtoScript II First Strand cDNA Synthesis (New England Biolabs). The sequences were then amplified ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized using ProtoScipt First Strand cDNA Synthesis Kit (New England Biolabs) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using the ProtoScriptII First Strand cDNA Synthesis Kit (NEB; E6560S), following the manufacturer’s protocol with the Random Primer Mix and the RNA denaturation step ...
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bioRxiv - Plant Biology 2021Quote: ... miR158 OE and pseudo-PPR OE (one of these libraries failed the quality control).Libraries were prepared using NEB Next Small RNA Library Prep Set (New England Biolabs) and sequencing was carried out using Illumina HiSeq 4000 in single-end mode by outsourcing ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... was used as an input for library preparation and libraries were prepared using NEB ultra II DNA library prep kit (NEB). Sequencing reads (76 bp PE ...
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bioRxiv - Cell Biology 2022Quote: ... Library construction: The RNA sequencing library was constructed using NEBNext® Ultra II RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligation product was amplified through 10 reaction cycles to generate a whole genome library (MC library) using NEBNext Ultra II DNA library Prep Kit for Illumina (New England Biolabs) and 750ng MC library was used for exome enrichment ...
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bioRxiv - Microbiology 2024Quote: ... The extracted nucleic acid was then subject to library construction using NEBNext Ultra RNA Library Prep Kit and NEB Next Ultra DNA Library Prep Kit (LTD.NEB, China) for RNA and DNA samples ...
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bioRxiv - Neuroscience 2022Quote: ... DNAse I treated RNAs were used to synthesize cDNAs using the ProtoScript cDNA First Strand cDNA Synthesis Kit (New England Biolabs Inc., USA). Primers for RT-PCR were designed to prevent amplification from genomic DNA (annealing sites in different exons or across intron-exon boundaries) ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described(54) ...
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bioRxiv - Microbiology 2019Quote: ... We prepared sequencing libraries with NEBNext Ultra DNA Library Prep kits (NEB) and sequenced them on an Illumina NextSeq with 2×150 paired end reads (mid-output run ...
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bioRxiv - Developmental Biology 2019Quote: ... Library quantification was performed with NEBnext Library Quant Kit (New England Biolabs), prior to paired-end sequencing on HiScanSQ (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... Final libraries were quantified using NEBNext Library Quant Kit for Illumina (NEB) and high sensitivity D1000 TapeStation (Agilent) ...
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bioRxiv - Cell Biology 2021Quote: ... libraries were prepared using the NEBNext Ultra RNA library Prep Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs Inc.) from 1 µg RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were prepared using the DNA Library Prep NEB (New England Biolabs) and sequenced in a NextSeq500 sequencer SE75bp (Illumina).
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bioRxiv - Molecular Biology 2020Quote: ... Library preparation (NEBNext Ultra RNA Library Prep kit, New England Biolabs®) and sequencing (HiSeq ...
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bioRxiv - Genomics 2019Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Kit (NEB) and sequenced on an Illumina Nextseq 500 platform (Department of Zoology ...
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bioRxiv - Genetics 2019Quote: ... Sequencing libraries were prepared using DNA Ultra II library preparation kit (NEB) according to the manufacturer’s instructions and sequenced either on Illumina’s HiSeq2000 Sequencer (50 bp single-end mode ...
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were then quantified by qPCR using NEBNExt Library Quant Kit (NEB) for Illumina on QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB), modified for CUT&RUN as previously described (48) ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed according to NEBNext Small RNA Library Prep kit (NEB) protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were prepared with an NEBnext DNA Library Prep kit (NEB, E7370L). Adaptor-ligated DNA was size-selected and purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... libraries were prepared using NEBNext Ultra II DNA Library (New England Biolabs) according to manufacturer’s instructions ...