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Citations for New England Biolabs :
501 - 550 of 2182 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: sgRNAs of the target gene were obtained by in vitro transcription as described in (Leonetti et al., 2016) with modifications only to the PCR polymerase used (Phusion; New England Biolabs, M0530S). A 100 μL reaction of mNG21_10 HL-60 cells were electroporated (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/μL was used for subsequent reverse transcription assay with Luna® Universal One Step RT-qPCR kit (E3005, New England Biolabs) according manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... were heated to 65°C and slow-cooled to 37°C before reverse transcription with 5 U AMV-RT (NEB, M0277L) at 42°C for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... and 1 μg of purified PCR product was used as template for in vitro transcription using the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S) as described by the manufacturer.
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bioRxiv - Evolutionary Biology 2020Quote: ... Reverse transcription was carried out via SuperScript IV and cDNA was posteriorly amplified using Q5® High-Fidelity DNA Polymerase (NEB) with the ARTIC nCov-2019 primers and following the recommendations in the sequencing protocol of the ARTIC Network (https://artic.network/ncov-2019) ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... The guide region was then amplified by PCR and paired guide RNAs synthesised by in vitro transcription (T7 Quick High Yield RNA Synthesis kit, NEB, #E2050S). Single stranded DNA oligonucleotides (WT oligo ...
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bioRxiv - Developmental Biology 2021Quote: ... Resultant DNA was gel extracted then 400 ng template used for in vitro transcription with the HiScribe RNA synthesis T7 kit (NEB, E2040) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... For this Reverse transcription was carried out via SuperScript IV and cDNA was posteriorly amplified using Q5® High-Fidelity DNA Polymerase (NEB) with the ARTIC nCov-2019 primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Toe-printing reactions were carried out in 5-µl aliquots containing a PURExpress transcription-translation coupled system (New England Biolabs, USA) to which the test template was added (16) ...
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: MNV plasmids were linearised with NotI and phenol/chloroform extracted before being used for in vitro transcription using the HiScribe™ T7 ARCA mRNA Kit (NEB), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The AvcI DNA template for in vitro transcription was PCR amplified from pAvcI using Q5 High-Fidelity DNA Polymerase (NEB™). To incorporate the T7 promoter into the final AvcI DNA template ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 µl PCR amplified product was used for the in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), incubating at 37 °C for 18 hours in the thermocycler ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting PCR product was purified by phenol-chloroform extraction and used for in vitro transcription using SP6 RNA-polymerase (NEB, M0207). The resulting sgRNA was purified using the High Pure PCR Cleanup Microkit (Roche ...
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bioRxiv - Bioengineering 2023Quote: mRNA was synthesized through in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, China, Cat: E2080S). The templates for in vitro transcription were linearized plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... When needed the mRNA was polyadenylated after transcription using a recombinant poly-A polymerase as recommended by the manufacturer (NEB Biolabs).
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bioRxiv - Microbiology 2023Quote: ... For in vitro transcription plasmids were first linearised by restriction digest and purified using Monarch PCR and DNA Clean-up Kit (NEB, USA). 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... a plasmid containing a full infectious clone of RV-M33 was linearized then added to an in vitro transcription reaction with Sp6 (New England Biolabs, M0207L). The resulting RNA was purified then polyadenylated (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A 2 µL aliquot of the annealed oligonucleotides was used as template for in vitro RNA transcription by HiScribe T7 quick high yield RNA Synthesis kit (New England Biolabs, MA). The transcribed RNA was purified using RNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Cell Biology 2022Quote: ... stop codon and 3’ T7 terminator were used as templates for the coupled in vitro transcription/translation PURExpress system (New England Biolabs, USA). The various SQS constructs used for cysteine crosslinking comprised an N-terminal 3xFLAG tag ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Ligated fragments were separated from free linkers on a 15% polyacrylamide TBE-Urea gel and then pooled and purified for reverse transcription using RT primer NI-802 and ProtoScript II Reverse Transcriptase (NEB; M0368S). The ∼105 nt cDNAs were separated from free RT primers on a 15% TBE-Urea gel and circularized using CircLigaseII ssDNA Ligase (Biosearch Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 µl of the PCR-amplified product was employed for in vitro transcription using the NEB HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, E2040S), with incubation at 37 °C for 18 hours in a thermocycler ...
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bioRxiv - Immunology 2024Quote: ... The sequences containing T7 promoter and sgRNA were PCR-amplified and used as templates to produce sgRNAs through in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). All sgRNAs were purified by lithium chloride precipitation and stored at −80°C.
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bioRxiv - Immunology 2024Quote: ... A total of 1µg of the purified PCR product was used for in vitro transcription using the HiScribe T7 High Yield RNA synthesis (NEB E2040S), as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: The Rapid Amplification of 5’cDNA end was carried out with Template Switching Reverse Transcription protocol (Wulf et al, 2019) provided by New England Biolabs (NEB #M0466). In the first step ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of PCR product was used in an in vitro transcription reaction using the HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) in the presence of 20units/mL SUPERaseIn ...
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bioRxiv - Neuroscience 2024Quote: ... the resulting product was purified via column purification and underwent further amplification and conversion to RNA by a high-yield in-vitro transcription reaction using T7 polymerase (NEB, E2040S). Subsequently ...
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bioRxiv - Biochemistry 2024Quote: ... in vitro transcription templates were prepared via 8 cycles of PCR using 2x Q5 Master Mix (New England Biolabs, Cat. M0492S) with a T7 promoter containing forward primer as previously described ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting amplicon was used as starting material for in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Finally ...
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bioRxiv - Biophysics 2021Quote: ... with 10 µl of folding mixture containing 10 nM M13mp18 scaffold DNA (New England Biolabs), 100 nM unmodified oligonucleotides (Integrated DNA technologies) ...
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bioRxiv - Genomics 2021Quote: - T4 gene 32 protein (10 mg/mL, NEB or EPFL “homemade” T4g32p, 10 mg/mL). In 96-well plates ...
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bioRxiv - Biophysics 2020Quote: ... with 10 μl of folding mixture containing 10 nM M13mp18 scaffold DNA (New England Biolabs), 100 nM unmodified oligonucleotides (Integrated DNA technologies ...
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bioRxiv - Genomics 2022Quote: ... 10 μL of 10 mg/mL BSA and 50 μL of T4 Ligase (NEB M0202L) in a total volume of 1000 μL with nuclease-free water ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% SDS) + 10 μL of 10 mg/mL Proteinase K (New England Biolabs, Cat # P8107S). DIP digestion buffer was added to input samples up to 200 μL ...
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bioRxiv - Genomics 2023Quote: ... Buffer 3.1 and 10 µL of 10 U/µL DpnII restriction enzyme (Cat#R0543T, NEB) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein blots were incubated with primary antibodies overnight at 4°C using the above-mentioned antibodies and the Gaussia Rabbit Polyclonal Antibody (E8023S, New England BioLabs), the Influenza A NS1 Mouse Monoclonal Antibody (sc-130568 ...
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bioRxiv - Microbiology 2024Quote: ... A 10 μL volume of 10-fold DNAse I reaction buffer (NEW ENGLAND BioLabs, catalogue-B0303S), 5 units (2.5 μL ...
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bioRxiv - Developmental Biology 2020Quote: ... The sgRNAs were synthesised by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Physiology 2019Quote: PCR templates generated as described above (see Table S3) were used for in vitro transcription reactions using the HiScribe T7 RNA Synthesis Kit (New England Biolabs, Whitby, ON) following the recommended conditions when using modified nucleotides ...
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bioRxiv - Microbiology 2021Quote: ... nucleic acid (5 μL) was used as template for reverse transcription using LunaScript® RT SuperMix Kit (New England Biolabs, Hitchin, UK) in 20 μL reaction volume ...
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bioRxiv - Cell Biology 2022Quote: ... The 1970 bp DNA was then gel purified and 500 ng of DNA template was loaded into a 20-μL in vitro transcription reaction with 5,000 units of T7 RNA polymerase (New England BioLabs, product number M0251LVIAL), 1X RNAPol Reaction Buffer (New England BioLabs ...
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bioRxiv - Immunology 2019Quote: ... Then sgRNA template for in vitro transcription (IVT) was prepared by PCR amplification of Phusion high fidelity DNA polymerase (NEB Biolabs, Ipwich, MA), the PCR mixture was cleaned up by PCR cleanup reaction (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA synthesis was performed by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription and qPCR were performed in a one-step reaction using Luna® Universal One-Step RT-qPCR (NEB, Ipswich MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs, Ipswich, MA, USA). Purified sgRNA (0.5 μg ...
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bioRxiv - Microbiology 2022Quote: ... Template for in vitro transcriptions was amplified from PM-infected tissue cDNA using Phusion polymerase (M0530S/L, New England Biolabs, Ipswich, Massachusetts) and the T7 promoter (5’-TAATACGACTCACTATAGGGGG-3’ ...