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Citations for New England Biolabs :
151 - 200 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... coli JM109 strain (New England Biolabs (NEB)) was used ...
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bioRxiv - Microbiology 2021Quote: ... coli strains are derivatives of DH5alpha (NEB).
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bioRxiv - Microbiology 2021Quote: ... coli strain BL21(DE3) (NEB Labs, C2527) was used for protein overexpression ...
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bioRxiv - Bioengineering 2022Quote: ... coli strain BL21 (DE3) (New England Biolabs). From each selection output 184 clones were selected and expressed in 96-well plates ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain NiCo21(DE3) (C2529H from NEB) harbouring the recombinant construct was grown in 1-2 L of LB medium with 40 μg/mL Kanamycin at 30°C until mid-log phase ...
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bioRxiv - Microbiology 2022Quote: ... a strain of dh10b (NEB, Intact Genomics) containing a Cas13-crRNA plasmid (Supplementary Table 2 ...
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bioRxiv - Microbiology 2022Quote: ... a strain of dh10b (NEB, Intact Genomics) containing a Cas13-crRNA plasmid (Supplementary Table 2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli DH5α strain (NEB5α, New England Biolabs). The plasmids were purified by a PureLink miniprep kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... coli strain 10-beta (New England Biolabs) or TOP10 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... coli strains are derivatives of DH5alpha (NEB). M ...
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bioRxiv - Cell Biology 2020Quote: ... coli strain BL21(DE3) (New England Biolabs), the bacteria were grown at 37°C to an OD600 of 0.5 ...
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bioRxiv - Cell Biology 2020Quote: ... coli BL21(DE3) strain (New England Biolabs). The recombinant protein expression was induced with 1mM IPTG in cells during 4 h at 25°C and the bacterial pellet was stored at −20°C until use ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli strain NEB10β (New England Biolabs, MA) was used for all cloning experiments ...
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bioRxiv - Microbiology 2022Quote: ... coli strain DH10B (New England Biolabs, UK) was used for transformation with the plasmid vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... coli strain BL21 (DE3) (New England Biolabs) was transformed with Pet151-Dyp-V5 and incubated over night at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... coli strain NEB5alpha (#C2987H, New England Biolabs) cultured overnight aerobically at 37°C in LB broth or solid LB agar ...
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bioRxiv - Synthetic Biology 2023Quote: Escherichia coli strain NEB5-α (NEB C2987) used for plasmid cloning were grown at 37 °C in lysogenic broth (LB ...
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bioRxiv - Plant Biology 2023Quote: ... coli strains NEB10 (New England Biolabs #C3019H) or NEB stable (New England Biolabs #C3040H ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain DH10B (# C3019I, New England Biolabs) and positive clones were selected in the presence of ampicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... coli strain (New England Biolabs, Beverly, MA). Bacteria clones were grown in Luria–Bertani (LB ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB-5α (New England Biolabs) was used for MES transformation when constructing plasmids as well as for plasmid propagation ...
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bioRxiv - Bioengineering 2023Quote: ... coli strain BL21(DE3) (New England Biolabs). From each of the selection outputs ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain BL21(DE3) (New England Biolabs) and grown on plates overnight at 37 °C (LB agar ...
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bioRxiv - Microbiology 2023Quote: ... coli strain BL21(DE3) (New England Biolabs) grown in either LB media ...
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bioRxiv - Molecular Biology 2023Quote: ... coli T7 Express strain (New England Biolabs) as described33.
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bioRxiv - Synthetic Biology 2023Quote: ... coli strain NEB10β (New England Biolabs, MA) was used for all cloning experiments ...
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bioRxiv - Biochemistry 2024Quote: ... E.coli BL21 (D3) strains (New England Biolabs) were transformed with a T7-driven effector plasmid and an E.coli constitutive promoter J23119 to drive sgRNA expression ...
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bioRxiv - Biochemistry 2021Quote: ... coli or Expi293™ cells underwent denaturation at 95 °C for 10 min in glycoprotein denaturing buffer (New England Biolabs) followed by immediate cooling on ice for 10 s ...
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Biochemistry 2021Quote: ... 100 U PNGase F (New England Biolabs) was lyophilized (for up to 5 μg trimer) ...
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bioRxiv - Biochemistry 2021Quote: ... The optimal PNGase F (Cat. P0709S, NEB) concentration (relative to the glycoprotein substrate ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with PNGase F (NEB) following the manufacturer’s instructions or mock treated as a control ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 µl PNGase F (NEB P0704S), 3µl 10% NP40 ...
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bioRxiv - Immunology 2020Quote: ... Enzymes PNGase F (P0704S, New England Biolabs) and Endo-Hf (P0703L ...
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bioRxiv - Biochemistry 2020Quote: ... PGNase F or EndoH (New England Biolabs) using the buffers and protocols provided by the supplier ...
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bioRxiv - Physiology 2019Quote: ... and 8 μl of PNGase F (NEB) were added ...
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bioRxiv - Cell Biology 2021Quote: ... or N-glycosilase F (PNGaseF, NEB P0704S), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... or PNGase F (P0704S, New England Biolabs) for 2 hours prior to electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: PNGase F digestions (New England Biolabs #P0704S) were performed according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 U PNGase F (New England Biolabs) was lyophilized ...
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bioRxiv - Cell Biology 2023Quote: ... and PNGase F (500U)(Cat# P7367, Biolabs) in 1 x GlycoBuffer-2 (Biolabs) ...
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bioRxiv - Cell Biology 2021Quote: ... The Spike ORF was subcloned into pcDNA3.1(+) vector via Gibson Assembly (NEB). Subsequently ...
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bioRxiv - Biochemistry 2022Quote: ... The thiolated spike-ins were made using T7 RNA polymerase (NEB M0251) on BamHI-digested templates ...
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bioRxiv - Microbiology 2023Quote: ... Spike chimeras were generated through Gibson assembly of PCR generated fragments (NEB). Primers used for site directed mutagenesis and Gibson assembly are described in table 1 ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was incubated with 2000 units of N-glycosidase F (PNGase F) (New England BioLabs) at room temperature for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were resuspended in PBS and in some cases treated with N-glycosidase F (PNGase F; New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... coli T7 expression strains C2566 (Dam+) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1 ...
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bioRxiv - Microbiology 2021Quote: ... coli strain MG1655 (ATCC 47076) and DH5α (NEB) were grown in LB or MMB (LB supplemented with 0.1 mM MnCl2 and 5 mM MgCl2 ...