Labshake search
Citations for New England Biolabs :
451 - 500 of 642 citations for Rubella Spike Glycoprotein E1 strain F Therien since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: The presence of N-linked oligosaccharides was examined using PNGase F (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2020Quote: ... together with controls were subject to enzymatic digestion by PNGase F (P0708L, New England Biolabs), Endo H (P0702L ...
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bioRxiv - Immunology 2020Quote: ... N-linked glycans were released from gp140 in-gel using PNGase F (New England Biolabs). The released glycans were subsequently fluorescently labelled with procainamide and excess label and PNGase F was removed using Spe-ed Amide-2 cartridges (Applied Separations) ...
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bioRxiv - Neuroscience 2021Quote: ... C or E antibodies and the immunoprecipitates were treated with PNGase F (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Biochemistry 2021Quote: ... acylated dasTMPRSS2 was treated with 50 U PNGase F (NEB; 37 °C for 45 min) to trim N-glycan branches ...
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bioRxiv - Cell Biology 2020Quote: ... Denatured cell lysate was then deglycosylated using 500 U of PNGase F (New England Biolabs) in 1x glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... Enzymatic assays for PNGase F and Endo S were performed according to manufacturer’s instructions (NEB). The effect of the enzymatic assays was either analyzed by Coomassie staining or immunoblotting.
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bioRxiv - Molecular Biology 2023Quote: ... Each pooled library was PCR amplified using Phusion polymerase (F-530) (New England Biolabs, M0530S). Quality of the libraries was assessed with a Bioanalyzer using the High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... for 10 min and treated with PNGase F or Endo Hf enzymes (New England BioLabs) for 1.5 h at 37°C in accordance with the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 and incubated with PNGase F (1,000 U, New England BioLabs, Ipswich, MA, USA) and Sialidase A (5 mU ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 500 U PNGase F (NEB, cat# P0704S), 500 U Endo H (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Strain specific abundance was assessed with the Luna® Universal qPCR Master Mix (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... coli BL21 (DE3) strain and purified with amylose agarose beads (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... subtilis strain bearing Physp-bdbDC at the ycgO locus (bJZ185) using LongAmp Taq DNA Polymerase (NEB) and oligonucleotide primer pair (oJZ436 + oJZ437) ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa strains using the primers indicated in Table S7 with Q5 DNA Polymerase (New England Biolabs), in reactions that added regions of homology to plasmid pUCP20 ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
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bioRxiv - Immunology 2022Quote: ... Delta and BA.2 spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described10.
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bioRxiv - Biochemistry 2020Quote: ... excised bands containing 20 μg of gp145 were incubated with 5000 units of PNGase F (NEB) for 24 hrs at 37°C [31] ...
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bioRxiv - Cell Biology 2022Quote: ... cleared lysate was incubated without or with PNGase F (NEB, 1 μl per 40 μl lysate) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... bromophenol blue) or subjected to a PNGase F digest before being supplemented with sample buffer (NEB, performed as suggested by the supplier) ...
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bioRxiv - Biochemistry 2021Quote: ... Concentrated medium was collected from the filters and 3 μl of PNGase F (New England Biolabs) was added to each sample then incubated at 37 °C for 24 h ...
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bioRxiv - Biochemistry 2022Quote: ... Deglycosylated cell lysates or conditioned media were prepared by treatment of PNGase F (New England Biolabs). Samples containing around 30 μg proteins was loaded to SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... In some cases immunoprecipitated proteins were treated with PNGase-F or Endo-H (New England Biolabs) according to the manufacturers protocols ...
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bioRxiv - Microbiology 2023Quote: ... cell and virus particle lysates were treated with PNGase F and Endo Hf (New England Biolabs) following the manufacturer’s protocol for 1 hour at 37°C prior to Western blotting.
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bioRxiv - Molecular Biology 2024Quote: The glycosylation status of the proteins was studied using the PNGase-F and the EndoH (NEB) glycosidases according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 µL of Rapid PNGase F (New England Biolabs, Fig. 2d and Extended Data Fig. 2a) were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... crassa strains expressing a Dam fusion was digested with 1 μL of DpnI (NEB, 20 units/μL); ligation to primer 5050 was carried out overnight at 16 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
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bioRxiv - Biophysics 2020Quote: ... we used an alternative strategy that enables us to propagate expression plasmids in standard bacterial strains (NEB 5-alpha Competent E ...
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bioRxiv - Biophysics 2020Quote: In vivo folding experiments were carried out in E.coli strain Lemo21(DE3) (New England Biolabs, NEB, C2528J). All plasmids used in this study are based on a backbone with a pUC origin ...
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bioRxiv - Biophysics 2020Quote: In vivo folding experiments were carried out in E.coli strain Lemo21(DE3) (New England Biolabs, NEB, C2528J). All plasmids used in this study are based on a backbone with a pUC origin ...
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bioRxiv - Microbiology 2022Quote: ... and dam−/dcm− (dam−/dcm− competent E. coli, #C2925) strains were purchased from NEB (Ipswich, MA, USA) and cultured in Luria-Bertani (LB ...
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bioRxiv - Synthetic Biology 2021Quote: The CFP and GFP reporter plasmids were propagated in either dam-/dcm-strain C2925 (New England Biolabs) or dam+/dcm+ strain DH5-alpha Turbo (NEB C2984 ...
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bioRxiv - Molecular Biology 2023Quote: ... hassiacum strain DSM 44199 as a template for colony-PCR with Q5 High-Fidelity DNA polymerase (NEB) to generate a fragment cloned into initial vector pINIT (Cm25) ...
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bioRxiv - Bioengineering 2023Quote: ... The sequence-confirmed plasmid was transformed into Escherichia coli strain BL21(DE3) chemically competent cells (NEB C2527) according to NEB’s transformation protocol and plated on LB plates containing 100 µg/ml carbenicillin.
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage products were then deglycosylated through treatment with 250 U PNGase F (New England Biolabs, Ipswich, MA) for 16 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified proteins were pre-treated (were indicated) with PNGase F or Endo H (New England Biolabs, USA) according to the manufacturer’s protocol before analysis by Western blotting using Strep-MAb-HRP classic antibody.
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bioRxiv - Microbiology 2019Quote: ... verticillioides with the primers FvGbb2-GFP-F/R using Q5® High-Fidelity DNA Polymerase (NEB: M0491S). The DNA fragment was introduced to the pKNTG plasmid KpnI and HindIII sites via In-Fusion-HD cloning kit (Takara Bio USA ...
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bioRxiv - Plant Biology 2021Quote: ... protein pellets were dissolved in 20 μl of PNGase F denaturation buffer (New England Biolabs, Ipswich, MA) and denatured at 95°C for 10 min ...
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bioRxiv - Biochemistry 2019Quote: ... Washed beads were incubated with 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) in a head-over-head rotator overnight at 37 °C.
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bioRxiv - Immunology 2021Quote: ... These three RBDs were also treated with PNGase-F (New England Biolabs, Ipswich, MA, USA; Cat# P0704S) following the manufacturer’s instruction and loaded onto SDS-PAGE gels to evaluate the impact of size caused by glycosylation ...
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bioRxiv - Biochemistry 2022Quote: ... PNGase F (P0704) and mass spectrometry grade Trypsin (Trypsin-Ultra, P8101) are from New England Biolabs (NEB). Fbs1-GYR proteins were expressed and purified from E ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 20μg protein was denatured following manufacturer’s protocol followed by PNGase F and O-Glycosidase & Neuraminidase Bundle (NEB) treatment at 37°C for 1 hour ...