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Citations for New England Biolabs :
101 - 150 of 1275 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: H4-SNAP histones were labelled with 3 μM TMR fluorophore (NEB) for 30 min in complete medium ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP was labelled with SNAP-Cell 647-SiR (from NEB Biolabs), Halo was labeled with Janelia Fluor 646 Halo ligand ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP was labelled with SNAP-Cell 647-SiR (from NEB Biolabs), Halo was labeled with Janelia Fluor 646 Halo ligand ...
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bioRxiv - Molecular Biology 2022Quote: 3’-end 32P-labelled gapped DNA was generated using TdT (NEB) and [α-32P]dATP (PerkinElmer) ...
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bioRxiv - Microbiology 2019Quote: ... labelled with 250 nM SNAP-Cell TMR-Star (New England Biolabs) and grown under anaerobic conditions for a further 60 min ...
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bioRxiv - Immunology 2022Quote: ... 5 mol% labelled with SNAP-Surface Alexa Fluor 488 (NEB, S9128S), to 25% v/v HeLa lysate in a final volume of 20 µl (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antisense oligonucleotides were 32P-labelled with T4 PNK (NEB) and used as probes ...
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bioRxiv - Genomics 2022Quote: ... and 30 nM recombinant Cas9 nuclease (NEB, M0386S) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... 1 μg of recombinant H4 (New England Biolabs) and 100 μM S-adenosyl methionine (SAM ...
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bioRxiv - Cell Biology 2023Quote: ... 100 or 500 units of recombinant CK2 (NEB), 5µM or 15µM silmitaserib (CK2 inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL 1U recombinant shrimp alkaline phosphatase (NEB) and 2.5 µL 10x CutSmart buffer (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), and 0.1 mg/ml RNase A (QIAGEN)] ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), 0.1 mg/ml RNase A (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Microbiology 2020Quote: ... The resulting labelled probes were purified on DNA Clean up columns (NEB) then incubated at 0.5 nM for 5 min at 37°C in EMSA Buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: 5’-end 32P-labelled DNA substrates were generated using T4 PNK (NEB) and [ɣ-32P]ATP (PerkinElmer) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were labelled in suspension with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and FACS-sorted to select for SNAP-GLP-1R-expressing ones ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
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bioRxiv - Genomics 2023Quote: ... dTTP and biotin-dATP using Klenow DNA polymerase I (NEB M0212L), followed by in situ proximity ligation at room temperature overnight ...
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bioRxiv - Biochemistry 2021Quote: ... This experiment was further repeated with recombinant Histones (NEB) (0.5µg ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant human FICD proteins (aa 104-445) were expressed as His6-Smt3 fusion constructs in T7 Express lysY/Iq (NEB) E ...
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bioRxiv - Molecular Biology 2021Quote: ... DIG-labelled probes were synthesized by in vitro transcription (New England BioLabs Inc.) with appropriate polymerases ...
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bioRxiv - Biophysics 2021Quote: ... Dynein-dynactin-adaptor complexes were labelled with 2 µM SNAP-TMR dye (NEB) during the isolation procedure from rat brain lysate (McKenney et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... rk430-mScarlet-SNAP (7 μM monomer) was incubated with benzylguanine-biotin (NEB) in a 4 to 1 molar ratio at room temperature for 15 minutes ...
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bioRxiv - Immunology 2021Quote: Purified recombinant CV3-25 IgG was mixed with LysC (NEB) at a ratio of 1μg LysC per 10mg of IgG and incubated at 37°C for 18h with nutation ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified recombinant DNA was treated with DpnI (NEB Biolabs) for 3 h ...
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bioRxiv - Plant Biology 2022Quote: ... Probes were end-labelled using [γ-32P]ATP and polynucleotide kinase (New England Biolabs), according to (Sambrook et al ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5′ end labelled using γ32P UTP (Perking Elmer) and T4 polynucleotide kinase (NEB) following manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... SNAP reagents (BG-649, BG-fluorescein and BG-biotin) were obtained from NEB. Electrocompetent cells were used in all transformations.
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bioRxiv - Biophysics 2023Quote: ... The biotin-modified DNA was digested using a KasI restriction enzyme (NEB #R0544S) that produced two fragments of 24 kb and 24.5 kb ...
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bioRxiv - Genetics 2019Quote: ... after the recombinant plasmids linearized with NotI Restriction Enzyme (NEB, USA), and then the capped mRNAs were purified by RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
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bioRxiv - Immunology 2021Quote: ... A similar dilution series of recombinant histone H3 (New England Biolabs) was prepared starting at 1 µg/ml protein ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM DTT with 1.5 µM recombinant H3 (New England Biolabs), vehicle or 500 nM recombinant enzyme ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant bacmids were created by transformation into DH10Bac competent cells (NEB), screening on XGAL plates ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Cell Biology 2019Quote: ... APC was eluted with APC purification buffer and labelled with SNAP-reactive dye (NEB#S9105S). After SNAP-labelling ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were labelled with SNAP-Surface-649 (0.5 µM, from New England Biolabs, Hitchin, UK) for 30 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... For size comparison a radioactively labelled DNA ladder (100 bp DNA ladder, New England Biolabs) was applied.
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bioRxiv - Plant Biology 2023Quote: ... The membrane was hybridised with radioactively labelled probes (T4 PNK, M0201, New England Biolabs (NEB)) in Ultrahyb buffer at 35 ᵒC and exposed to a phosphor screen that was scanned using Typhoon scanner (Cytiva).