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Citations for New England Biolabs :
51 - 100 of 1275 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag of the VWF-dimer constructs in a bulk reaction in the presence of 5 μM sfp phosphopantetheinyl transferase53 and 10 mM MgCl2 at 37 °C for 60 min ...
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bioRxiv - Biophysics 2020Quote: CoA-biotin (New England Biolabs) was coupled to the ybbR-tag at the C-terminus of the fusion protein constructs in a 90 min bulk reaction in the presence of 4 µM sfp phosphopantetheinyl transferase63 and 100 mM MgCl2 at room temperature (≈ 22°C) ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Cell Biology 2022Quote: Recombinant human ADAMTSL2 constructs where generated by PCR-amplification with the Q5 Hot start high fidelity 2x master mix (NEB) and specific primer pairs to allow for restriction cloning ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W. Cai) and 0.03 U/μl T4 DNA polymerase (NEB, #M0203) at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... labelled with Alexa647-SNAP dye (NEB) was continuous imaged using 100 ms exposure.
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... EcoRI sites underlined) and different concentration of recombinant His-SET8 protein (0 to 1.4 μM, New England Biolabs) were incubated for 10 min on ice in 1× GRB binding buffer [20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Purified sgRNAs targeting tlnrd1 and slc45a2 were pooled separately and complexed with recombinant Cas9 protein (New England Biolabs) in vitro using 300mM KCl buffer for 5 minutes at +37°C ...
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2022Quote: SNAP–tagged kinesin-8B-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C overnight ...
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bioRxiv - Cell Biology 2019Quote: SNAPf–tagged Kinesin-8X-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C for 1.5 hrs ...
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bioRxiv - Biophysics 2023Quote: Biotin-SNAP-mDia1 prepared by incubating purified SNAP-mDia1 with Benzylguanine-Biotin (New England Biolabs, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... After annealing the complex an equimolar amount was mixed with 1000ng Cas9 recombinant protein (NEB; final conc 20ng/ul) and incubated at RT for 15’ ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Genetics 2019Quote: P2C-Cas9 and P2C-EGFP proteins were expressed from pET28a-P2C-Cas9 and pRSET-P2C-EGFP respectively by recombinant BL21 E.coli (NEB) as described in detail in Chaverra-Rodriguez et al ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μg each of the recombinant proteins were lyophilized and digested with PNGase F (P0701S, New England Biolabs Inc.) according to the manufacture’s instruction ...
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bioRxiv - Biophysics 2020Quote: ... the biotin-Chd1p was prepared by mixing the SNAP-Chd1p and SNAP-biotin® (New England BioLabs) in a 1:2 molar ratio and incubated at room temperature for 30min ...
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... was inserted into a published oligonucleotide scaffold (Talbot and Amacher, 2014) and injected together with recombinant Cas9 protein (New England Biolabs) into 1-2 cell stage zebrafish (AB strain) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... coli C2523 pMAL-c5X vector and recombinant MUP (rMUP) was made using pMAL Protein Fusion and Purification System (New England Biolabs) using methods similar to prior studies27,36 ...
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bioRxiv - Developmental Biology 2019Quote: ... The recombinant DBINO domain containing protein (~80kDa) was purified using the amylose affinity column (New England Biolabs, Massachusetts, United States) and detected using the anti-MBP-HRP antibody (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: 32P-labelled DNA fragments were generated by PCR using primers labelled with [γ-32P]ATP using T4 polynucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of 10 mM biotin (NEB) was then added to each sample on the plate and incubated for an additional five minutes to compete away purely biotin-dependent interactions ...
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bioRxiv - Biochemistry 2023Quote: ... and labelled using SNAP-Surface AlexaFluor 488 (NEB) using manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: DNA ends were marked with biotin-14-dATP by adding 60μL of biotin fill-in master mix (1X NEB 3.1 ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: ... 1 μl of 1 mM BG-biotin or BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 μl of the purified axonemes in HMEEK buffer and incubated overnight at 4 °C ...
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bioRxiv - Systems Biology 2021Quote: ... the purified DNAs were annealed using random nonamer primers with a 5′-biotin tag (5′-Biotin-CTACACGACGCTCTTCCGATCTNNNNNNNNN-3′) in the presence of Klenow fragments (3′-5′ exo-, New England Biolabs). Then ...
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bioRxiv - Bioengineering 2022Quote: Recombinant Cas9 (New England Biolabs, USA) was used to generate a standard curve (20µM ...
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bioRxiv - Physiology 2022Quote: ... Recombinant CK2 was purchased from NEB. Recombinant TGFBR1 was purchased from Proqinase ...
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bioRxiv - Plant Biology 2021Quote: ... oligos were end-labelled using T4 polynucleotide kinase (NEB) with γ-32P ...
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bioRxiv - Genetics 2023Quote: ... that was labelled using Klenow Fragment exo- (NEB, M0212L) with Digoxigenin-11-dUTP alkali-stable (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... labelled for old (SNAP-Cell® TMR-Star, NEB) and young organelles (SNAP-Cell® Oregon Green ...
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bioRxiv - Synthetic Biology 2020Quote: ... BG-fluorescein or BG-biotin conjugates (known as “SNAP-Surface® 649”, “SNAP-Cell® Fluorescein” and “SNAP-Biotin” respectively, NEB, at a 1:500 dilution ...
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bioRxiv - Genetics 2023Quote: ... using a biotin-labeled 30 μM dNTP mix (dATP, dGTP, dTTP and Biotin-14-dCTP Thermo Fischer, 19518018) and Klenow enzyme (NEB, M0210L) at 37°C for 80 min ...
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bioRxiv - Molecular Biology 2021Quote: ... SET8 recombinant enzyme (New England Biolabs # M0428S) was incubated with recombinant active PARP1 (Trevigen # 4668-100-01 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3µl recombinant AsiSI endonuclease (10U/µL, NEB) was added to three biological replicates and incubated (2 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (all NEB) at 37°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... and recombinant shrimp alkaline phosphatase (rSAP, NEB) overnight and PCR purified (Qiagen QIAQuick PCR Purification Kit ...
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bioRxiv - Systems Biology 2023Quote: ... 0.2 mg/mL recombinant albumin (NEB B9200S), 1 μM RT primer ...
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bioRxiv - Microbiology 2022Quote: ... and end-labelled with T4 polynucleotide kinase (New England Biolabs). This fragment was then digested for 2 h with MfeI at 37°C in a reaction volume of 60 μl ...
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bioRxiv - Microbiology 2023Quote: ... Oligonucleotide 5S-rRNA-oligo was labelled using T4 PNK (NEB) and [γ-32-P]-ATP (Hartmann Analytics ...
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bioRxiv - Genomics 2020Quote: ... Biotin labeled dATP (Thermo,19524016) and Klenow (NEB, M0210) were used to fill restriction fragment overhangs ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...