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Citations for New England Biolabs :
351 - 400 of 639 citations for Polybrominated Diphenyl Ether Surrogate Spiking Stock 10X Solution 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Ligation was done overnight at 16°C also rotating at 900rpm for 10 seconds every 5 minutes by adding 120µl of 10X ligation buffer (NEB), 664µl water ...
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bioRxiv - Neuroscience 2022Quote: ... the DNA was first cut by mixing the plasmid with a 10x digestion buffer (NEBuffer; Biolabs; Ipswich, MA, USA), a 10x EcoRI restriction enzyme (Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... samples were incubated for 5 min on ice and 3 μL of 10X G5 buffer (New England BioLabs, UK) were added to the samples together with 1.5 μL of 25X concentrated protease inhibitor cocktail (Roche ...
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bioRxiv - Genomics 2022Quote: ... 2.5μg of gDNA was dephosphorylated by the addition of 5μl of 10X NEB Cutsmart® buffer and 1μl of QuickCIP (NEB) in a total volume of 48μL ...
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bioRxiv - Bioengineering 2023Quote: ... NEB RNAPol reaction buffer (M0251S; 10X) and yeast inorganic pyrophosphatase (YIPP; M2403S; 0.1 U μl−1) were purchased from New England Biolabs (NEB). RNase H was purchased from ThermoFisher Scientific (EN0201 ...
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bioRxiv - Molecular Biology 2023Quote: ... 37°C with rotation) in 100 µL MNase reaction mix (87 µL ddH2O, 10 µL 10x MNase buffer, NEB, 1 µL 100x BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were washed in CutSmart buffer, and incubated (1h, RT) in 150 µL blunting mix (112.5 µL ddH2O, 15 µL 10x blunting buffer, NEB, 15 µL 100 µM dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: Purified and concentrated mTAAR7f was mixed with about 10x molar excess of both the heterotrimeric G protein and Nb35 and 0.5 U apyrase (NEB) and incubated overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Plain linear DNA was incubated with 10x molar excess of each hairpin oligo in a Golden Gate Assembly (NEB). Subsequently ...
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bioRxiv - Genetics 2023Quote: ... digested pJFRC12-10XUAS-IVS-myr::GFP backbone using the following reactions conditions: 4 µL T4 ligase buffer (10x) (NEB), 20 µl plasmid backbone DNA (0.005 pmol) ...
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bioRxiv - Genetics 2023Quote: To each tube/sample was added 20 μL of 10X MNase buffer (New England Biolabs Inc.; Ipswich MA, USA), 0.5 μL of 1M MgCl2 ...
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bioRxiv - Genetics 2023Quote: ... 8.5 μl of phosphorylated product was combined with 1 μl of 10x T4 ligase buffer and 0.5 μl of T4 DNA ligase (NEB), incubated at 16℃ overnight ...
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bioRxiv - Genomics 2024Quote: For a typical blunting reaction 5-50 ng of cfDNA are mixed with 5 μl of CutSmart 10x (NEB), 5 μl of dNTPs 1mM each ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB) in a 20 μL reaction for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.5 µL of 10X GlycoBuffer 3 (Cat.#B1720S; New England Biolabs) and 2.5 µL of Endo Hf in a total reaction volume of 25 µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.6 µL of 10X GlycoBuffer 2 (Cat.#B3704S; New England Biolabs), 2.6 µL of 10% NP-40 (Cat.#B2704S ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP ...
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bioRxiv - Molecular Biology 2024Quote: ... A-tailing was performed by addition of 5 µL of the A-tailing mix (0.5 µL of Cutsmart buffer 10X, 0.25 µL Klenow 50 U/µL (NEB, M0212M), 0.05 µL dATP 100 mM ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Microbiology 2022Quote: Viral stocks were pretreated with Dnase I (10U/ml, New England Biolabs Inc, MA, USA) and Benzonase nuclease (50U/ml ...
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bioRxiv - Immunology 2022Quote: Virus stocks were generated by either transfecting purified in vitro transcribed viral RNA (HiScribe, NEB) or transfecting the infectious cDNA clone containing plasmid in BHK-T7 cells ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 μM of complementary primers containing 20 bp overlap - target sequence - and 4 bp of homology to the expression vector were hybridized in a reaction containing 10 μL of 10x T4-ligase buffer (New England Biolabs). The mixture was submitted to 70 decreasing cycles of a minute each - touchdown - from 95 to 25°C and then connected to the previously linearized vector through a reaction containing 15 ng of the previous hybridization ...
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bioRxiv - Molecular Biology 2020Quote: Oligonucleotide was annealed before use: 20 μl 100 pM/μl oligonucleotide and 20 μl 10x T4 DNA ligase buffer (NEB) in 200 μl final volume were incubated in a heating block 95°C 5 min then block was removed from heat and left to cool to room temperature over ~2 h.
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bioRxiv - Genomics 2019Quote: The circularized DNA was split into 8 50 ul rolling circle amplification (RCA) reactions (5 ul 10x Phi29 buffer (NEB), 2.5 ul 10 mM dNTPs (NEB) ...
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bioRxiv - Genomics 2019Quote: ... Beads were washed twice with SPRI wash buffer and resuspended in 200 ul of intra-aggragete mix (171 ul water, 1 ul 100 mM ATP, 20 ul 10x NEB T4 DNA Ligase Buffer ...
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bioRxiv - Genomics 2019Quote: ... Beads were washed twice with SPRI wash buffer and resuspended in 50 ul of end fill-in mix (37 ul water, 5 ul 10X NEB Buffer 2 ...
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bioRxiv - Genomics 2020Quote: ... 5 μl of 10X AT buffer and 3 μl of Klenow enzyme from NEBNext® dA-Tailing Module (E6053L, NEB) was added to the sample ...
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bioRxiv - Cancer Biology 2019Quote: ... Ligation of DNA ends between the cross-linked DNA fragments was performed by addition of 992 μL of ligation master mix (150 μL of 10x ligation buffer (B0202S, NEB), 759 μL sterile water ...
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bioRxiv - Genomics 2019Quote: ... The ligase was killed by heating at 65°C for 10 min and then 5 µl of 10x reaction buffer 3 (NEB), 34 µl of H2O and 1 µl of PstI (NEB ...
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bioRxiv - Genomics 2021Quote: ... 10ng of cDNA is circularized using a DNA splint compatible with 10X cDNA and the NEBuilder HIFI DNA Assembly Master Mix (NEB). The DNA splint was generated by primer extension of the following oligos:
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bioRxiv - Genomics 2019Quote: ... The first 60 μL PCR reaction (also run as three parallel 20 μL reactions) included 6 μL of 10X ThermoPol Taq buffer (NEB), 0.96 μL of 5 μM forward primer (0.08 μM final) ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of genomic DNA was fragmented in a 20-μl reaction consisting of 2 μl of 10X fragmentase buffer (New England Biolabs) and 2 μl fragmentase (New England Biolabs ...
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bioRxiv - Synthetic Biology 2021Quote: ... The annealing was carried out in 96-well PCR plates by mixing 2 μL of each oligonucleotide at 100 μM with 2 μL of 10x T4 DNA Ligase Reaction Buffer (NEB) and 14 μL of water ...
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bioRxiv - Cancer Biology 2021Quote: ... Ends were subsequently ligated by adding a 900 μL master mix containing 120 μL 10X T4 DNA ligase buffer (NEB), 100 μL 10% TritionX-100 ...
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bioRxiv - Neuroscience 2022Quote: ... Ligation was done overnight at 16°C also rotating at 900 rpm for 10 seconds every 5 minutes by adding 120 µl of 10X ligation buffer (NEB), 664 µl water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1000 ng of DNA diluted in 27 μL of nuclease-free water was dephosphorylated by the addition of 3 μL of 10X CutSmart Buffer and 3 μL of Quick Calf Intestinal Phosphatase (NEB) and then incubated at 37ºC for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... To seventeen microliters of the eluates were added 2 μL of 10x Nucleoside Digestion Mix reaction buffer and 1 μL of Nucleoside Digestion Mix (New England Biolabs). Reactions were incubated at 37 °C for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligation was set up at room temperature with the addition of the following reagents: 100 μL of 10X T4 DNA ligase buffer (NEB), 10 μL of 10 mg/mL BSA and 50 μL of T4 Ligase (NEB M0202L ...
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bioRxiv - Cancer Biology 2021Quote: ... For cloning the oligo pool into the appropriate lentiviral backbone the following reaction was set up: 5 μl 10x Cutsmart buffer (NEB), 1 mM DTT (final) ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated blunt ends were then ligated using a ligation reaction (663μl water, 120μl 10X NEB T4 DNA ligase buffer (NEB), 100μl 10% Triton X-100 (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Non-encapsidated nucleic acids were removed by mixing 900 μL of each sample with 100 μL 10x DNase buffer and supplemented with 2 μL (4 U) of DNase I (NEB) and 1 U of RNase ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Chromatin was then digested by adding 12 μl of 10x NEBuffer3.1 and 8 μl of 5 U/μl DpnII (NEB, R0543) followed by a 2h incubation at 37ºC in a ThermoMixer with shaking (900 rpm ...
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bioRxiv - Genetics 2019Quote: ... This was subsequently added to 10x volume of modified binding buffer (Allentoft et al. 2015) and passed through a Monarch silica spin column (NEB) by centrifugation (Supplementary Methods Section 1 and Supplementary fig ...
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bioRxiv - Developmental Biology 2019Quote: ... Mixed diluted primers (1.7 μl) were combined with 1 μl of annealing buffer (10X NEBuffer 4, New England Biolabs Inc.) on ice in reaction tubes ...
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bioRxiv - Genetics 2019Quote: ... DNA samples were incubated at 20°C for 4h without rotation in a mix of 10 µl of 10x NEB2 buffer (New England Biolabs), 1 mM of a dNTPs mix (10 µl) ...
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bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...