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Citations for New England Biolabs :
251 - 300 of 639 citations for Polybrominated Diphenyl Ether Surrogate Spiking Stock 10X Solution 13C12 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 2.5 μL of Taq Buffer B (Mg-free; 10X) (New England Biolabs, Ipswich, MA, USA), 2.5 μL of dNTPs (2.5 mM of each base) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was removed by adding 2 μL DNaseI and 5 μL 10x DNase buffer (NEB) to the purified RNA and incubated at 37C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... consisting of 150 μL 10X NEB T4 DNA ligase buffer with 10 mM ATP (NEB), 125 μL 10% Triton X-100 ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... at specific sequence motifs (GCTCTTCN^) in the presence of 1 μl 10X buffer 3.1 (NEB) for 2 h at 50 °C and ultra-pure water to a volume of 10 μl ...
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bioRxiv - Microbiology 2022Quote: ... PCR master mix reagents (see Figure 4): Taq polymerase and 10X Reaction Buffer (NEB M0273S), nucleotide mix containing 10 mM dTTP ...
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bioRxiv - Molecular Biology 2023Quote: ... This was combined with 1.5 µl 10X T4 DNA ligase buffer (New England Biolabs Ltd.), 1.5 µl 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Plant Biology 2023Quote: Each PCR consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... 10 u/μL T4 DNA ligase (stock 400 u/μL, NEB). No difference in yield was found between the ligation splints ...
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bioRxiv - Cancer Biology 2019Quote: ... CviQI restriction enzyme (RE; 2.5U; 10U/μl stock, New England BioLabs), the TERT assay ...
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bioRxiv - Bioengineering 2022Quote: ... 0.042 µL Bst 2.0 DNA polymerase (120,000 U / ml stock, NEB), 0.1 Ribolock RNAse Inhibitor µL (40 U / µL stock ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 µl of PCR product was then mixed with 1.5 µl 10X NEBuffer 2 (B7002S, NEB) and 1.5 µl of Nuclease-free water ...
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bioRxiv - Bioengineering 2020Quote: A 10x Cas12-gRNA complex mixture was prepared by pre-incubating 1 μM of LbCas12a (NEB) with 1.25 μM gRNA in 1x NEBuffer 2.1 at 37°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... Exonuclease I mix (20 μL of 10X Exonuclease I buffer (New England Biolabs, Ipswich, MA, USA), 10 μL of Exonuclease I (New England Biolabs) ...
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bioRxiv - Molecular Biology 2019Quote: ... a master mix was prepared containing 2 µl 10x T4 polynucleotide kinase buffer without ATP (NEB) and 1 µl murine RNase inhibitor per sample and 3 µl were added to each sample together with 2 µl truncated T4 polynucleotide kinase (#M0201 ...
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bioRxiv - Molecular Biology 2021Quote: ... This was followed by the addition of 1 µl 10x T4 RNA ligase reaction buffer (NEB), 1 µl NEB T4 RNA Ligase2 ...
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bioRxiv - Microbiology 2020Quote: ... End repair reactions were conducted by adding 10 μL of 10x T4 DNA ligase buffer (NEB), 4 μL of 10 mM dNTPs ...
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bioRxiv - Immunology 2022Quote: ... 10 μl PCR products were incubated with 1.5 μl 10X NEBuffer (New England Biolabs, Ipswich, MA) and 1.5μl Nuclease-free water with the thermal cycler setting ...
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bioRxiv - Systems Biology 2022Quote: ... Each 20μL GoldenGate reaction consisted of 2μL of 10X T4 DNA Ligase Reaction Buffer (NEB #B0202S), nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... and 1 uL of 10X T4 DNA ligase reaction buffer (New England Biolabs, Cat. No. B0202S) in a final volume of 10 uL ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR products (∼300 ng) were incubated with 2 μl 10X NEBuffer 4 (New England Biolabs), 1 μl BtsCI restriction enzyme ...
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bioRxiv - Genomics 2024Quote: ... the cfDNA can be de-phosphorylated by adding 6 μl of Antarctic Phosphatase buffer 10x (NEB), 2 μl of Antarctic Phosphatase Enzyme (NEB) ...
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bioRxiv - Bioengineering 2022Quote: ... 0.3 µL Warmstart ® RTx reverse transcriptase (15,000 U / ml stock, NEB), 1 µL 10x LAMP primer mix (20 µM FIP and BIP ...
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bioRxiv - Genomics 2022Quote: ... Nonspecific adenine methyltransferase EcoGII (New England Biolabs, high concentration stock 25,000U/mL) was added to the reaction and incubated at 37 for 30 min with 300rpm shaking every 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... 1 µL of MNase (stock at 25 U/µL) (New England Biolabs) and 63 µl of water ...
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bioRxiv - Biophysics 2022Quote: ... 2 μL of a 0.4 µg µL-1 stock of Trypsin (NEB) were added to the samples (to a final enzyme:substrate ratio of 1:50 w/w ...
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bioRxiv - Synthetic Biology 2023Quote: ... Restriction digest using BsmBI v2 (NEB, stock concentration 10 000 U/mL) was performed at a BsmBI-v2 concentration of 0.67 U/μL ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 μl of 10x restriction enzyme buffer and a total of 400 units of DpnII (NEB, R0543) were added to the sample ...
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bioRxiv - Genomics 2020Quote: ... 8.5 uL of this phosphorylated product was combined with 1 uL of 10X T4 ligase buffer (NEB) and 0.5 uL of T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2019Quote: ... by adding 20 µL of ligation mix [2 µL of [10X Taq DNA ligase buffer (NEB B0208S), 0.25 µL Taq DNA ligase (NEB M0208S) ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then resuspended in 125 µL of ice-cold 10X NEB2 Buffer (New England Biolabs), and again spun down at 13,000 x g for 5 minutes at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions conducted at UR-MAI consisted of 50 μl reactions: 5 μl 10X Standard Taq Buffer (NEB), 2.5 μL of each primer ...
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bioRxiv - Microbiology 2020Quote: ... The reverse transcription reaction (20 μL) contained 2 μL 10x M-MulV buffer (B0253S, New England Biolabs), 1 μL of 50 μM Oligo 18 dT (SO132 ...
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bioRxiv - Genomics 2021Quote: ... The cell suspension was then mixed with 5 ml ligation buffer (550 μl 10x ligation buffer (NEB), 27.5 μl 20 mg/ml BSA (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... then 30 units of Mung Bean Nuclease were added along with 10x Mung Bean Nuclease buffer (NEB) to a final concentration of 1x ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.
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bioRxiv - Genomics 2021Quote: ... 90uL of the short RNA elution fraction was polyadenylated using 12uL 10x polyA polymerase buffer (NEB, B0276), 12uL 10mM ATP (NEB ...
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bioRxiv - Genomics 2021Quote: ... 8.5 µL of this phosphorylated product was combined with 1 µL of 10X T4 ligase buffer (NEB) and 0.5 µL of T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µl of 10x Promega T4 ligase buffer (contains ATP) with 1µl of PNK kinase (NEB) and incubated at 37°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Add 948 μl of ligation master mix: 150 μl 10X NEB T4 DNA ligase buffer (NEB, B0202), 125 μl 10% Triton X-100 ...
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bioRxiv - Genomics 2022Quote: ... 18μl of dNTP mix 2.5mM each and 18μl of 10x ligation buffer (New England Biolabs cat. #B0202S) and incubating for 30 minutes at 20ºC.
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bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
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bioRxiv - Synthetic Biology 2022Quote: ... Endonuclease restriction digests were done to a 20µl volume (5µl plasmid DNA, 2µl 10X cutsmart buffer (New England BioLabs), 0.5µl enzyme and pure water ...
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bioRxiv - Bioengineering 2022Quote: Packaged AAV vectors were first combined with equal volume of 10X DNase buffer (New England Biolabs, B0303S) and 0.5 µL 10 U/µL DNase I (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... ligation master mix was added (10x NEB T4 DNA ligase buffer with 10 mM ATP B0202-NEB); 10% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... Dephosphorylation was performed by adding 10X Antarctic phosphatase buffer and 0.5 µL of Antarctic Phosphatase (NEB M0289S) prior to incubation for 2 h at 37 °C ...