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Citations for New England Biolabs :
401 - 450 of 4860 citations for NK 1 Receptor Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cleaved and purified DNA was purified by PCI and ethanol precipitation and was subjected to dA-Tailing using Klenow Fragment (NEB, no. E6053). Reactions were incubated for 30 min at 37 °C and immediately subjected to adapter ligation as per the manual of Oxford Nanopore Ligation-based library prep kit (ONT ...
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bioRxiv - Microbiology 2023Quote: ... purified and subjected to ligation with the digested and gel-purified PCR product utilizing T4 DNA ligase (New England Biolabs, Cat. # M0202S).
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bioRxiv - Microbiology 2021Quote: ... Oligonucleotide probes were end-labeled with γ-32P ATP (Perkin-Elmer) and T4 PNK (New England Biolabs) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
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bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... the cells were first labeled with cell-permeable substrate SNAP-Cell Fluorescein (New England Biolabs, Cat. # S9107S) overnight at 240 nM concentrations ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: FLAG-TEV-SNAP-Ago2 expressed in HEK293T cells was labeled with SNAP-Surface Alexa Fluor 647 (NEB) and immunopurified by an anti-FLAG antibody conjugated onto Dynabeads Protein G (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq DNA Polymerase (NEB, #M0267) for one hr ...
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bioRxiv - Molecular Biology 2019Quote: ... 400 ng of eluted DNA fragments were end-labeled with T4 Polynucleotide Kinase (T4 PNK) (NEB M0201L) and 10 µCi of [α-32P]-ATP (Perkins Elmer NEG002A ...
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bioRxiv - Microbiology 2020Quote: ... suspended in 200 µL of phosphate saline buffer (PBS) and labeled with TMR-Star (New England Biolabs) for 30 min at 37°C in the dark at a final concentration of 250 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... The glpFKD promoter fragments were labeled with 32P using DNA Polymerase I Large Fragment (Klenow fragment) (NEB) to fill in the 5’ overhang with [α-32P]dATP ...
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bioRxiv - Developmental Biology 2023Quote: ... Digoxigenin-labeled antisense Alk3 RNA probe was prepared by linearization with EcoR1 (New England Biolabs, Ipswich, MA) and transcription with T7 RNA polymerase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit anti-DYKDDDDK (#D6W5B; NEB) and rabbit anti-myc (#71D10 ...
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bioRxiv - Synthetic Biology 2022Quote: ... rabbit anti-SNAP-tag (NEB; P9310 ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Cancer Biology 2021Quote: ... After purified with Monarch RNA Cleanup Kit (NEB Biolabs), RNAs were polyadenylated with E ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then further purified over amylose resin (NEB) and eluted with elution buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... and then 2.2 nmol purified streptavidin (New England BioLabs) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified RNA was fragmented with RNA Fragmentation Buffer (NEB) at 95°C for 5 min ...
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bioRxiv - Genetics 2020Quote: ... 2μg purified plasmids were digested with KpnI/XbaI (NEB) and ligated with a KpnI/XbaI-digested fragment containing a promoter and reporter gene (EGFP) ...
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bioRxiv - Genetics 2020Quote: Purified Rrp1 was incubated with ssDNA (Phi X174, NEB) or dsDNA (Phi 174 RF I ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Plant Biology 2020Quote: ... MBP-PUB25/26 were purified using Amylose Resin (NEB) by batch purification according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... and then purified with the Monarch PCR kit (NEB). PCR amplicons were digested using MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Microbiology 2022Quote: ... and purified using Monarch PCR clean-up kit (NEB). Blunted DNA was A-tailed using NEBNext dA-tailing kit (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmids and DNA were purified using Monarch kits (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... A pre-assembled complex of purified Cas9 protein (NEB) and gRNA was injected and the efficiency of Crispr/Cas9-induced mutagenesis in the F0 generation monitored at 24 hpf using a T7 endonuclease assay (Jao et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... purified and digested with NlaIII overnight (New England Biolabs). After a second ligation step ...
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bioRxiv - Microbiology 2022Quote: PCR amplicons were purified using Thermolable Exonuclease I (NEB), diluted at a ratio of 1:2 and amplified following the Nextera XT Index protocol (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... Purified plasmid was linearized with BamHI-HF (NEB 3136), and DNA concentration was quantified using a Nanodrop spectrometer (Thermo scientific) ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... The resulting purified plasmids were digested (New England BioLabs), ligated ...
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bioRxiv - Plant Biology 2020Quote: ... 10 μM of purified proteins mixed with PEG8000 (NEB) at 10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... MBP-MYB6 protein was purified on amylose resin (NEB) and HIS-PUB26 ...
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bioRxiv - Synthetic Biology 2020Quote: Purified RNA was reverse-transcribed with MuLV-R (NEB) and Random Primer Mix (NEB ...
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bioRxiv - Microbiology 2021Quote: ... 5 μg purified LbCas12a (New England BioLabs, catalog# M0653T) was incubated with equal molar purified gRNA (near 0.5 μg ...
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bioRxiv - Synthetic Biology 2020Quote: The protein samples of purified ribosome (New England BioLabs), E ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified RNA was fragmented with RNA Fragmentation Buffer (NEB) at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR product was purified on silica columns (NEB T1034) and assembly with Nluc_HBB_3UTR fragment was performed as described above for initial preparation of IVT template using HBB29_N35 amplicon ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR product was purified on silica columns (NEB T1034) and amplified with under the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... OmpR and OmpS* were purified by amylose resin (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The complexes were further purified on amylose resin (NEB). Fractions containing protein were pooled and diluted in 3 volumes of Amylose buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... and purified nascent RNA using streptavidin beads (NEB, S1421S). Next the nascent RNA was decapped using RppH (NEB ...
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bioRxiv - Genomics 2021Quote: ... biotinylated DNA fragments were purified by streptavidin beads (NEB). In step 6 ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with the Monarch PCR & DNA Cleanup Kit (NEB) and ligated with T4 DNA ligase (NEB) ...