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Citations for New England Biolabs :
551 - 600 of 4860 citations for NK 1 Receptor Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The transcription products were then purified (New England Biolabs, T2050L) and the cap1 tructure was added for efficient translation and evading the cellular innate immune response (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The RNA samples were purified by treating with DNaseI (NEB) to remove the genomic DNA contamination ...
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bioRxiv - Genomics 2023Quote: ... The 3 library pools were purified by an ExoSAPII (NEB) reaction to remove single-stranded DNA and further by column purification (MiniElute Gel Extraction Kit ...
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bioRxiv - Cell Biology 2023Quote: ... The mRNA was purified by Monarch RNA Cleanup Kit (NEB). Purified mRNAs were stored at 2-6 μg/μl concentration and were diluted prior to injection with Femtojet to 100-500 ng/μl.
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... Transcripts were purified with a Monarch RNA purification kit (NEB) and quantified with a Qubit™ RNA broad-range assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified using the Monarch® DNA gel extraction kit (NEB) and subjected to Sanger Sequencing for ORF sequence-verification prior to codon-optimization at Genscript and subcloning ...
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bioRxiv - Microbiology 2019Quote: ... rabbit α-Gaussia Luc (E80235, NEB), rabbit α-PB1 (PA5-34914 ...
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bioRxiv - Molecular Biology 2022Quote: ... and rabbit anti-myc (#71D10; NEB) primary antibodies were used ...
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bioRxiv - Molecular Biology 2021Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... 1 pmol of DNA template was mixed with 10 pmol of oligonucleotide 9 labeled with Yakima Yellow and 1µL of HemoKlen Taq DNA Polymerase (New England Biolabs) in a 6µL reaction mixture containing 50 mM Tris-HCl pH 9.0 ...
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bioRxiv - Biochemistry 2021Quote: ... S121A or triple mutant (AAA)) was incubated and labeled in vitro with 2U of CK2 (New England Biolabs, P60105) in a 30µl reaction containing 3pM adenosine triphosphate (ATP) ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides complimentary to the target were end-labeled with γ-32P-ATP by T4 polynucleotide kinase (New England Biolabs). Labeled oligonucleotides were added to the membrane and incubated overnight at 45°C ...
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bioRxiv - Biochemistry 2019Quote: ... All sens RNA strands were labeled at their 5’ end with [γ-32P] ATP using protein nucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L), separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Biophysics 2023Quote: RNAs were labeled with γ32P-ATP using T4 polynucleotide kinase (PNK) according to the manufacturer conditions (New England Biolabs). Radioactive RNAs were separated from any non-incorporated γ32P-ATP via denaturing 15% polyacrylamide (19:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Genomics 2023Quote: ... cells in the latter plate were labeled with HaloTag Oregon Green and SNAP-Cell 647-SiR (New England Biolabs) simultaneously according to the manufacturers’ protocols ...
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bioRxiv - Biophysics 2023Quote: ... We then sequentially labeled Nup96 proteins with SNAP ligand conjugated Alexa Fluor 647 (BG-AF647, S9136S, New England Biolabs) and mitochondria with primary anti-Tomm20 antibodies (rabbit polyclonal ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was labeled by fill-in of EcoRI overhangs with the DNA polymerase I Klenow fragment (New England Biolabs) in the presence of [α-32P] dATP (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
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bioRxiv - Plant Biology 2023Quote: ... a CP29A specific rabbit antisera (Kupsch et al., 2012) or anti-SNAP rabbit antisear (NEB, Frankfurt, Germany) in 1:2000 dilution overnight at 4°C and subsequently washed with TBST buffer four times at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific], 38 μM dCTP, dGTP and dCTP [Thermo Fisher Scientific], 50 U Klenow Polymerase [NEB], 1x NEB 2 buffer) and cells incubated for 1 h at 37 °C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were spun down and resuspended in fill-in mix (biotin-14-dATP [Thermo Fisher Scientific], dCTP, dGTP and dTTP [Thermo Fisher Scientific], Klenow Polymerase [NEB], 1x NEB 2 buffer) for 1.5 h at 37°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA from the polyclonal parasites that returned from transfection was digested with BamHI and SpeI (New England Biolabs) and transferred to membrane (Nytran SuPerCharge ...
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bioRxiv - Biochemistry 2020Quote: ... then RNAs were purified using a Monarch RNA Cleanup kit (NEB), or homemade equivalent buffers and regenerated columns,75 following the same rationale ...
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bioRxiv - Cell Biology 2019Quote: ... MBP-MKLP1 proteins were purified on amylose resin (New England Biolabs), washed 5 times with Washing Buffer (50 mM Tris-HCl pH 7.5 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... MBP-fusion proteins were purified using Amylose resin (New England Biolabs), washed in lysis buffer ...
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bioRxiv - Genetics 2021Quote: ... mRNA was purified using NEB Monarch RNA cleanup kit (NEB, USA). 3 nl of 40 pg mRNA of each arm of the FusX mitoTALE-BE (left-pT3-FusXTBE-N and right-pT3-FusXTBE-C ...
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bioRxiv - Genomics 2021Quote: ... DNA was purified using Monarch® PCR & DNA Cleanup Kit (NEB), using the following recommended protocol modification ...
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bioRxiv - Biochemistry 2021Quote: ... Then the RNA was purified by Monarch RNA cleanup kit (NEB).
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bioRxiv - Bioengineering 2021Quote: ... using 66ng of purified genomic DNA and 10U HindIII-HF (NEB) in ddPCR Supermix for Probes (BioRad) ...
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bioRxiv - Bioengineering 2021Quote: ... The mRNAs were purified using a Monarch RNA Cleanup Kit (NEB). We amplified target RNAs and mRNAs using primers that include a T7 promoter sequence on the 5′ end ...
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bioRxiv - Bioengineering 2021Quote: ... The synthesized RNA was purified using Monarch RNA cleanup kits (NEB). Purified RNA was then heated at 65 °C for 5 min to denature the secondary structure ...
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bioRxiv - Bioengineering 2021Quote: ... Purified and denatured RNA was capped using Vaccinia Capping Kit (NEB) and finally purified using Monarch RNA cleanup kit.
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were gel purified and cloned via Gibson assembly (NEB) into pQCXIP-TRIM5α-HA of the matching species ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was purified using the Monarch PCR & DNA Cleanup Kit (NEB), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Biochemistry 2022Quote: ... purified NSD intermediates were digested with 0.4 U/μl AlwnI (NEB) in CutSmart Buffer (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... purified DNA was digested with BamHI-HF (New England Biolabs, R3136S) and SnaBI (New England Biolabs ...
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bioRxiv - Systems Biology 2022Quote: ... BCKDK was purified by its MBP tag on amylose resin (NEB).
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (NEB) for all fragments ...
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bioRxiv - Microbiology 2022Quote: ... Purified adaptor-DNA fragments were amplified by Phusion polymerase (NEB#M0530) in combination with specific primers (PCR1 Tn-spe direct and PCR1 Adaptor comp.) ...
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bioRxiv - Biochemistry 2019Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified plasmid was subsequently cut using either EcoRI-HF (NEB) or SapI-HF (NEB ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids were purified and analyzed by BstEII digestion (New England Biolabs) for confirmation of correct construction and electroporated into electrocompetent E ...
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bioRxiv - Synthetic Biology 2021Quote: ... Purified DNA was then treated with additional RNaseA/T1 mix (NEB) for 30 minutes at 37C and then single stranded DNA was isolated from the prep using an ssDNA/RNA Clean & Concentrator kit from Zymo Research ...
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bioRxiv - Microbiology 2021Quote: ... and purified using Monarch PCR clean-up kit (New England Biolabs). Blunted DNA was A-tailed using NEBNext dA-tailing kit and column-purified ...
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bioRxiv - Biochemistry 2020Quote: ... purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs, USA) and cloned into PET-24a (+ ...