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Citations for New England Biolabs :
301 - 350 of 4860 citations for NK 1 Receptor Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... Gel-purified cDNA products were ligated to the adapter oWG920 (Supplementary Table 6) using T4 RNA ligase 1 (NEB M0437M). cDNA cleanup was performed using 10 µl MyOne Silane beads (Thermo Scientific 37002D ...
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bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
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bioRxiv - Microbiology 2022Quote: ... 1 μg of purified IE1 DNA was then digested with ClaI and SpeI restriction enzymes (New England Biolabs, Ipswich, MA). Digested DNA was cleaned using the DNA clean and concentrator kit (Zymo Research ...
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bioRxiv - Biophysics 2022Quote: ... the construct was digested with EcoRV to eliminate ungapped fragments10 analyzed on a 1 % agarose gel and purified (Monarch DNA gel extraction kit, New England BioLabs). The final dsDNA constructs were 8’750 base pairs long with biotin attached on one end ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μg of purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and random hexamer primers to obtain 50ng/μl cDNAs.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then mixed with 350 μl 1× RNA protection reagent and RNA was purified using a Monarch total RNA extraction kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... To do this 2.5 uL of cutsmart buffer was added to 20 uL of purified PCR product and then 1 uL of DpnI (NEB) was added ...
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bioRxiv - Cancer Biology 2019Quote: ... Biotin was removed from non-ligated restriction fragment ends by incubating 40μg of DNA with T4 DNA polymerase (NEB) for 4 hours at 20°C in the presence of dATP and dGTP ...
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bioRxiv - Molecular Biology 2019Quote: ... we incubated the Pol II EC(CPD) (with and without biotin crosslinking) with 2μl streptavidin magnetic beads (NEB, USA) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled RNA probes were synthesized using T7 or T3 RNA polymerases (NEB, USA) with the DIG RNA Labeling Mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides were 5-end labeled with 32P-γATP using T4 polynucleotide kinase (New England Biolabs). Supercoiled pUC19 plasmid dsDNA was prepared by a method that did not involve DNA denaturation (Clewell and Helinski ...
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bioRxiv - Genomics 2020Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for 1 hr at 72° ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
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bioRxiv - Biochemistry 2019Quote: ... the helicase loading strand was end-labeled with [γ-32P]ATP by T4 PNK (NEB) using the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Each DNA probe was end labeled with γ32P-ATP with T4 PNK (New England Biolabs). Excess nucleotide was removed using G-50 microcolumns (GE Life Technologies) ...
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bioRxiv - Genomics 2021Quote: ... PCR fragments were labeled using 32P-γ-ATP with T4 polynucleotide kinase (New England, Biolabs). Protein–DNA complexes were incubated for 1h at room temperature in binding buffer containing 25 mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... which was purchased from Integrated DNA Technologies and end-labeled with T4 polynucleotide kinase (NEB) and γP32-ATP (6000 Ci/mmol ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently 5’32P-labeled using [γ-32P]-ATP and T4 polynucleotide kinase (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Biophysics 2020Quote: ... and labeled with 10 μM SNAP-Cell® TMR-Star (New England Biolabs Inc. #S9105S) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... U-labeled second-stranded DNAs were treated with heat-labile UDG enzyme (New England Biolabs), and ligated products were amplified with PCR by the following conditions ...
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bioRxiv - Genomics 2019Quote: ... The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq polymerase (NEB) for one hour at 72°C ...
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bioRxiv - Biochemistry 2021Quote: ... The lambda DNA ladder was labeled by filling in with Klenow fragment (New England Biolabs) in the presence of [α-32P]dATP ...
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Genetics 2023Quote: ... Synthetic oligonucleotides were labeled with [γ 32P]-ATP and T4 polynucleotide kinase (New England Biolabs) at their 5’ end ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... Radioactively labeled probes were generated by annealing complementary EMSA oligonucleotides (45 µM, NEB2 buffer (NEB), heating to 95° for 2 min and cooling down to 25° with −1°C per minute) ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 45 min and the supernatant was loaded onto amylose affinity resin (New England BioLabs, Ipswich, MA). The column was washed with 10 CVs of lysis buffer and the protein was eluted with sequentially added 1 CV aliquots of lysis buffer Supplemented with 20 mM maltose ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibodies used in this study were rabbit anti-GLuc 1:2,000 (E8023S, New England Biolabs), rabbit anti-epidermal growth factor receptor (EGFR) ...
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bioRxiv - Microbiology 2019Quote: ... Typhimurium NCTC 12023 and the vector including the Tet-on system aph tetR PtetA present on p4392 occurred using oligonucleotides as listed in Table S 1 and purified by PCR purification (NEB Monarch). The PCR product encoding for sadBA and the PCR product from vector p4392 were assembled by Gibson assembly according to manufactureŕs protocol (NEB Monarch) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... and subsequently purified by extraction from a 1% agarose gel using the Monarch DNA Gel Extraction Kit following manufacturer instructions (NEB # T1020S). The partially degenerate DNA oligonucleotide ...
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... Twelve restriction enzyme digests were prepared by combining 15 µL of each purified DNA sample with 1 µL DpnI (NEB R0176S) and 4 µL 10X CutSmart Buffer (NEB ...
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bioRxiv - Microbiology 2022Quote: ... A polyadenylated fraction was purified from the total RNA (1 µg) using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). This fraction was used to construct cDNA library using a previously described method [91] ...
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bioRxiv - Immunology 2023Quote: ... The digested and purified inset and vector were ligated at a ratio of 5:1 using T4 DNA ligase (NEB, M0202) overnight at 18°C ...
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bioRxiv - Cancer Biology 2023Quote: ... These products were then purified by separation on a 1.0% agarose gel at 100 volts constant for 1 hour and were then purified via the Monarch DNA gel extraction kit (NEB, #T1020L). Purified products were quantified with qubit high sensitivity dsDNA kit (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... The digest product was run on a 1% agarose gel and the band was excised and purified using a Monarch DNA Gel Extraction Kit (NEB #T1020) following the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged at 13000 x g for 1 minute and the supernatant was once more purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs). We expect the “crush and soak” method to improve signal strength if low labeling densities are used during extension.
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bioRxiv - Genetics 2024Quote: ... Anti-rabbit (#7074, NEB) and anti-mouse (#7076 ...
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bioRxiv - Genetics 2021Quote: ... 1 μg of total Trizol-purified RNA purified was used for library preparation using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB) with libraries indexed in 12 cycles PCR ...
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bioRxiv - Cell Biology 2023Quote: ... His-MBP-Mis18BP120-130 was purified using the same lysis buffer containing 500 mM NaCl and purified using amylose resin (NEB). Proteins were then eluted by an elution buffer containing 10 mM Maltose.
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bioRxiv - Molecular Biology 2019Quote: A 1 kb long DNA fragment with biotin molecules attached to the 5′ termini (dibiotin-DNA) was generated by PCR amplification using Q5 High-Fidelity Polymerase (NEB), with pAM075 as a template and the 5′-biotinylated primer pair oAM091/oAM092 ...
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bioRxiv - Biochemistry 2022Quote: SNAP–tagged kinesin-8B-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C overnight ...
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bioRxiv - Biophysics 2022Quote: ... the purified 8.2 kb DNA linker was annealed to the oligo DNA handle with 3’-biotin (5’-TGGACTGATGCGGTATCTGCGATATCCTA CGCAGGCGTTT-3’-biotin) in PBS at room temperature for 3 hr followed by purification using Monarch DNA purification kit (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... Klenow was then used to fill in restriction fragment overhangs and mark the DNA ends with biotin (M0210, New England Biolabs). Proximity ligation contact (PLC ...
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bioRxiv - Cell Biology 2019Quote: SNAPf–tagged Kinesin-8X-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C for 1.5 hrs ...
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bioRxiv - Genomics 2020Quote: ... the resulting synthetic RNA/DNA hybrid fragment was ligated to both the 600 and 400 bp DNA fragments before ligating a loop (PS359) and Y-shape (triple-biotin/PS866) at either end using T3 DNA ligase (NEB). The hairpins were gel purified and attached to paramagnetic MyOne T1 streptavidin beads (Dynal/Life Tech).
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bioRxiv - Immunology 2023Quote: ... was biotinylated with sulfosuccinimidyl-6-[biotinamido]-6-hexanamido hexanoate (sulfo-NHS-LC-LC biotin; ThermoScientific) and coupled to streptavidin beads (New England Biolabs). Patient samples were incubated with RBD-coupled beads and excess sera washed off with PBS (Sigma) ...