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Citations for New England Biolabs :
1 - 50 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: Codon-optimized human TDP-43-His LIC-A constructs were transformed into T7 express (New England Biolabs, #C3029J) E ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... dsRNA was synthesized from T7-linked DNA using the HI Scribe™ T7 High Yield RNA Synthesis Kit (New England, Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2020Quote: ... linearized and transcribed in vitro using the Hi-Scribe T7 High Yield RNA Synthesis Kit (NEB, Ipswich, MA). GAPDH IVT-RNA was purified by precipitation with lithium chloride followed by column purification using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... T7 exonuclease (T7 exo) (NEB#M0263L) and LbCas12a (NEB#M0653S ...
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bioRxiv - Microbiology 2023Quote: ... was used as a template for in vitro transcription using Hi-T7 RNA Polymerase and Ribonucleotide Solution Mix (both from New England Biolabs) in accordance with the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... T7 RNA polymerase (T7 RNAP, New England BioLabs) was added to a final concentration of 0.8 U/μL ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: ... using T7 RNA polymerase (HiScribe T7 kit, New England Biolabs) according to manufacturer’s instructions (E ...
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bioRxiv - Immunology 2020Quote: ... or Shuffle T7 (NEB) cell lines and purified using cation exchange followed by size exclusion chromatography.
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bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
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bioRxiv - Bioengineering 2021Quote: ... coli T7 express (NEB) containing a plasmid expressing the methylase Ple1 ...
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bioRxiv - Cell Biology 2022Quote: ... Shuffle T7 express (NEB), Origami B (DE3) ...
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bioRxiv - Genomics 2023Quote: ... a T7 endonuclease (NEB) digestion was performed and incubated at 37°C on a thermal shaker at 1000 RPM for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... T7 ligase (NEB, #M0318S) and nuclease free water ...
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bioRxiv - Genomics 2023Quote: ... T7 DNA ligase (NEB) and BsmbI (NEB).
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bioRxiv - Bioengineering 2024Quote: ... coli T7 Express (NEB). Colonies were picked into 10 mL LB containing either 100 µg/mL ampicillin (pDEST14/pETDuet ...
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bioRxiv - Cell Biology 2022Quote: ... 1/10 T7 RNA polymerase mix (HighScribe T7 High Yield RNA synthesis NEB)) at 37°C ...
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bioRxiv - Genomics 2019Quote: ... and T7 DNA ligase 30U/μL in T7 ligase buffer (New England BioLabs). The mixture was incubated at 25°C for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant human FICD proteins (aa 104-445) were expressed as His6-Smt3 fusion constructs in T7 Express lysY/Iq (NEB) E ...
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bioRxiv - Biochemistry 2023Quote: ... T7 RNAP@BG or T7 RNAP@PEG in RNA polymerase buffer (New England Biolabs). For reaction time courses ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 ligase (NEB, M0318S). The new gRNA constructs were packaged into lentiviral particles and transduced into SAM TSCs by direct supplementation of the lentivirus for 24h ...
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bioRxiv - Molecular Biology 2021Quote: ... T7-EI (New England Biolabs) assays were carried as is described in literature [26] ...
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A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activitybioRxiv - Biochemistry 2019Quote: ... T7 exonuclease (New England Biolabs), Exonuclease III (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL T7 Ligase (NEB) or 0.5 µL T4 Ligase (NEB) ...
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bioRxiv - Bioengineering 2019Quote: T7 Express lysY/Iq (NEB)
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bioRxiv - Cancer Biology 2020Quote: ... T7 Endonuclease 1 (NEB, M0302) was added to the mixture and incubated at 37°C for 15 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... with T7 RNA polymerase (NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... and T7 ligase (NEB, M0318S). The product from the Golden Gate reaction was transformed into Stbl3 competent cells (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: T7 endonuclease assays (M0302L, NEB) were performed following the manufacturer instructions.
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bioRxiv - Biophysics 2019Quote: ... 1 µL T7 polymerase (NEB), 5 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... coli T7 Express LysY (NEB) using electroporation and a 1 mm electroporation cuvette ...
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bioRxiv - Developmental Biology 2020Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Biochemistry 2022Quote: ... coli T7 Express cells (NEB). Isolated transformants were grown at 37°C until an OD600 of 0.8 was reached ...
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bioRxiv - Biochemistry 2022Quote: ... coli T7 Express cells (NEB). The libraries pre- and post-selection were then grown for 3 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli T7 Express cells (NEB). Cells were grown in 50 mL LB medium supplemented with kanamycin (50 μg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5µL T7 DNA ligase (NEB), 1.2µL 50µM N7 oligo (5’-CAGCACGGCGAGACTNNNNNNNNNNGACTTGTC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... and T7 ligase (NEB, M0318), using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... and T7 ligase (NEB, M0318), using 5 ng of PCR product and 50 ng of the CROPseq backbone (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... and T7 ligase (NEB, M0318). The product was precipitated with isopropanol and resuspended in 3 μl nuclease-free water (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... The T7 endonuclease I (NEB) was then added to the heteroduplexes and incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 200U T7 RNA polymerase (NEB), 50U Murine RNAse inhibitor (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... coli Shuffle T7 strain (NEB) was transformed with pET17b OVA-ZE ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Developmental Biology 2020Quote: A digoxin-labeled human H19X probe was transcribed in situ from a linear template plasmid by T7 RNA polymerase (NEB, UK) with Digoxin-labeled Uridine (Roche ...
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bioRxiv - Developmental Biology 2020Quote: ... and T7 RNA polymerase from HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Cold-anesthetized female mosquitoes were microinjected with 2.0 µg gene-specific dsRNA using a Nanoject II microinjector (Drummond Scientific Company ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL T7 RNA Polymerase mix (HiScribe T7 High Yield RNA Synthesis Kit, NEB) were incubated at 37°C for 3 h ...
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bioRxiv - Microbiology 2021Quote: ... The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013 ...
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bioRxiv - Bioengineering 2022Quote: ... mRNA was generated by in vitro T7 transcription (HiScribe™ T7 ARCA mRNA Kit, NEB). DNA and mRNA at the indicated concentrations were added to the mixture of cells and RT-PCR reagents immediately before proceeding to heat lysis.