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Citations for New England Biolabs :
101 - 150 of 8886 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from three pooled killing assay spots (as detailed in the previous section) utilizing the Monarch® Genomic DNA Purification Kit (NEB). Quantification of genomic DNA copies was targeted at the kdpAB gene and carried out with the Naica® Crystal Digital PCR System using Sapphire chips (Stilla Technologies).
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bioRxiv - Genomics 2020Quote: ... a dsDNA HS qubit assay and also by a NEBNext library quantitation Assay (New England Biolabs) before sequencing on Illumina’s HiSeq2500v4
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bioRxiv - Developmental Biology 2021Quote: ... The T7E1 assay (Cat#M0302, NEB) was then applied as in (Sato et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutants selected for cloning were also analysed via T7 endonuclease I-based (T7E1) heteroduplex assay according to the manufacturers protocol (EnGen® Mutation Detection Kit, NEB #E3321), and the length of digested and undigested fragments were visually compared by gel electrophoresis (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... as well as wildtype (WT) N2aC were assayed for mis-matched DNA pairs via T7 endonuclease assay (EnGen Mutation Detection Kit; New England Biolabs, Ipswich, MA) with PCR primers targeted towards our genomic region of interest (Integrated DNA technologies ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 fmol of DNA (determined following quantification with the Qubit™ dsDNA HS Assay Kit) was processed using the NEBNext Companion Module for ONT Ligation Sequencing (NEB, #E7180). Sequencing adapters were added using the Ligation Sequencing Kit (ONT ...
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bioRxiv - Cancer Biology 2022Quote: ... We designed spCas9 guide RNAs to target codon 12 of the human KRAS gene and selected the one with highest targeting efficiency determined by T7E1 mismatch assay (EnGen Mutation Detection Kit, New England Biolabs, Ipswich, MA). Truncated guide RNA was produced by PCR assembly of a guide RNA template (56 ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Genetics 2022Quote: ... T7 Endonuclease I assay (T7EI; NEB M0302L) or restriction enzyme digestion using Mse I (NEB R0525L) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we used the T7E1 assay (NEB #E3321) to determine potential mutations for every single fly following the protocol in (Zhu et al. ...
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bioRxiv - Cell Biology 2023Quote: ... a T7 endonuclease I cutting assay (NEB) was used to identify the extent of insertions/deletions for each guide ...
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bioRxiv - Molecular Biology 2023Quote: ... coli transposition assays were treated with AvrII (NEB) to reduce the proportion of contaminating pDonor ...
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bioRxiv - Microbiology 2024Quote: ... and the PNGase F assay (New England Biolabs) was completed following the manufacturer’s instructions using 15 µg of protein ...
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bioRxiv - Genetics 2021Quote: ... followed by T7 Endonuclease I Assay (New England Biolabs). 30 PCR cycles were run in all samples ...
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bioRxiv - Neuroscience 2019Quote: ... Editing activity was validated by the T7E1 assay (NEB) in rat C6 glioma cells (ATCC) ...
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bioRxiv - Neuroscience 2019Quote: ... T7 Endonuclease I assay (M0302, New England Biolabs Inc.) and Sanger sequencing (GENEWIZ ...
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bioRxiv - Neuroscience 2020Quote: ... we performed a T7 endonuclease I (T7EI) assay (NEB) on approximately 10 F1 embryos from a clutch to confirm whether any mutations had occurred in the germline of the injected parent ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) to detect indel mutations ...
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the T7 Endonuclease I (T7EI) assay (NEB).
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bioRxiv - Developmental Biology 2024Quote: ... restriction enzyme digest assay with BtsCI (New England Biolabs) was performed to identify WT ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Developmental Biology 2022Quote: ... 5’-CTTTTGACATCCGCTTCTGC-3’ followed by a T7 endonuclease assay (NEB) to detect indels ...
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bioRxiv - Developmental Biology 2020Quote: ... Kinase assays were performed using recombinant murine c-Abl (NEB). The amounts of GST-fusion protein to add to the reaction were calibrated using western blots of the purification products with mouse anti-NICD (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... All assays were done in Escherichia coli (strain: NEB Turbo) following the same plasmid protection assay described previously ...
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bioRxiv - Cell Biology 2019Quote: ... Deglycosylation assay using PNGase F (P0704S, New England BioLabs Inc.) was performed according to manufacturer’s protocol with the exception of incubating the reaction for 3 hours at 37°C followed by overnight at RT ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Developmental Biology 2020Quote: ... Mutations were detected through a T7 endonuclease I assay (NEB)(76) ...
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bioRxiv - Microbiology 2023Quote: Assays were performed using the PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... was confirmed using a Lambda phosphatase assay (New England BioLabs).
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bioRxiv - Bioengineering 2024Quote: DNA cutting assays were performed following New England Biolabs (NEB) method.39,40 Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... The assays are composed of 1X Isothermal Buffer (NEB #B0537), 6 mM Magnesium Sulfate (MgSO4 – NEB #B1003) ...
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bioRxiv - Neuroscience 2021Quote: ... cell viability was determined utilizing an XTT assay (New England Biolabs). Similar results were obtained utilizing calcium phosphate transfection methods ...
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bioRxiv - Cell Biology 2019Quote: ... Genome indel was assessed by T7 Endonuclease I assay (NEB #M0302) followed by SDS-PAGE gel electrophoresis (Genscript #M42012L) ...
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bioRxiv - Microbiology 2020Quote: ... we used a colorimetric assay using p-Nitrophenyl phosphate (pNPP) (NEB) as a substrate ...
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bioRxiv - Microbiology 2022Quote: ... and subsequent restriction enzyme assay of exon 5 (with BSaaI, NEB) and exon 7 (BSErI ...
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bioRxiv - Neuroscience 2023Quote: The assay was carried out following the manufacturer’s instructions (NEB, P0753S). Details are given in SI Appendix.
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bioRxiv - Synthetic Biology 2022Quote: ... To perform the qPCR assay 0.95 µL qPCR mastermix (M3003, NEB) containing 2 ng/µL template DNA was dispensed using a nanoliter dispenser (Cobra 4-channel dispenser ...
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bioRxiv - Molecular Biology 2023Quote: ... The optimized assay reactions comprised 1x CutSmart buffer (New England BioLabs) (20 mM Tris-acetate pH 7.9 ...
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bioRxiv - Developmental Biology 2023Quote: ... We then performed a T7E1 nuclease assay (M0302, New England Biolabs) according to the manufacturer’s protocol and resolved digested products on a 1.2% agarose gel containing STBYTM safe DNA gel stain (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cell viability was determined utilizing an XTT assay (New England Biolabs). 10 µL of unfractionated conditioned media or 5 µL of the 2K ...
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bioRxiv - Bioengineering 2020Quote: To prepare the DNA template for curtain assays λDNA (125 μg, NEB) was incubated with 2 μΜ biotinylated oligo complementary to one of the two 12 nt cohesive ends in λDNA in T4 DNA ligase reaction buffer (NEB ...
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bioRxiv - Microbiology 2021Quote: All plasmid protection assays were done in Escherichia coli (strain: NEB Turbo). As described previously12 ...
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bioRxiv - Molecular Biology 2023Quote: ... 400ng of gDNA was used for T7 endonuclease assay (NEB Cat#M0302). Assays were run in TBE polyacrylamide gels and visualized using Gel Red.
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bioRxiv - Cell Biology 2022Quote: ... Endo H assay was performed following manufacturer’s instructions (New England Biolabs, P0702S). Following addition of Glycoprotein Denaturing Buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR assays were performed using the Luna Universal qPCR Master Mix (NEB) in a MasterCycler RealPlex 4 (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: Amylose pulldown assay was performed with Amylose Sepharose beads (New England BioLabs) in a pulldown buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR assays were conducted with Luna Universal qPCR Master Mix (New England Biolabs). Primers for qRT-PCR analyses are listed in Table S4 ...