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Citations for New England Biolabs :
1 - 50 of 8886 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... ERK (9102, NEB, 1:10000); phospo-ERK (9101 ...
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bioRxiv - Neuroscience 2023Quote: ... phospo-ERK (9101, NEB, 1:10000); AKT (9272 ...
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bioRxiv - Microbiology 2020Quote: ... after phosphorylation using T4 Polynucleotide kinase (NEB). The constructs were then transformed into chemically competent DH5α E ...
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bioRxiv - Genetics 2023Quote: ... followed by 5′ phosphorylation (New England BioLabs), repurification ...
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bioRxiv - Genetics 2023Quote: ... phosphorylation by T4 polynucleotide kinase (NEB M0201S), and ligated with T4 DNA ligase (NEB M0202 ...
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bioRxiv - Genetics 2019Quote: ... After phosphorylation (T4 Polynucleotide Kinase, New England Biolabs) and annealing by temperature ramping from 95°C to 30 s by −0.1°C/min increments ...
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bioRxiv - Molecular Biology 2022Quote: ... and re-phosphorylation by T4 polynucletide kinase (NEB). For the oxidized sRNA library ...
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bioRxiv - Evolutionary Biology 2020Quote: ... phosphorylation with T4 Polynucleotide Kinase (New England BioLabs), and circularization with an NEB Quick Ligation Kit (New England BioLabs) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... phosphorylation with T4 Polynucleotide Kinase (New England BioLabs), and circularization with an NEB Quick Ligation Kit (New England BioLabs) ...
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bioRxiv - Genomics 2020Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, M0201L), (4 ...
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bioRxiv - Cell Biology 2022Quote: Phosphorylation was performed on bead using PNK (NEB, M0201L) and washed once with wash buffer (20 mM Tris-HCl pH 7.4 ,10 mM MgCl2,0.2% Tween-20) ...
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bioRxiv - Plant Biology 2022Quote: ... Following phosphorylation with T4 polynucleotide kinase (New England BioLabs), the PCR products were ligated into expression vector pQE-1 (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 5’ phosphorylation with T4 Polynucleotide Kinase (NEB) and self-ligation with T4 DNA Ligase (NEB).
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bioRxiv - Genetics 2021Quote: ... sgRNA oligos following phosphorylation were annealed by T4PNK (NEB, M0201) and inserted into a digested pCFD3 backbone by T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... followed by phosphorylation with T4 polynucleotide kinase (New England Biolabs). The vector pPEPZ-sgRNAclone was digested with BsmBI and carefully purified by gel extraction to ensure removal of the mCherry fragment ...
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bioRxiv - Biochemistry 2021Quote: 5’ phosphorylation of micro HHrz RNA with polynucleotide kinase (NEB) as done following manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: ... Phosphorylations and ligations were performed using T4 PNK kinase (NEB) and T4 DNA ligase (NEB) ...
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bioRxiv - Plant Biology 2023Quote: ... after which an anti-p42/p44-erk antibody (NEB) was employed to detect the activated MAPKs on western blots.
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bioRxiv - Molecular Biology 2021Quote: ... followed by phosphorylation of the 5’ end with T4 kinase (NEB). Phosphorylated RNA was then purified by the RNeasy kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Molecular Biology 2020Quote: 5’ end phosphorylation (1x PNK buffer, 20 u T4 PNK (NEB), 80 u RNaseOUT ...
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bioRxiv - Genomics 2022Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
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bioRxiv - Biochemistry 2022Quote: ... CXCR7 phosphorylation site mutants were generated by site-directed mutagenesis using Q5 Site-Directed Mutagenesis Kit (NEB, Cat. no. E0554S). For the NanoBiT assay ...
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bioRxiv - Microbiology 2023Quote: ... 5’ phosphorylation of RNA fragments was achieved using T4 Polynucleotide Kinase (NEB) and Adenosine 5’-Triphosphate (ATP ...
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bioRxiv - Biophysics 2023Quote: ... followed by 5’-end phosphorylation with T4 PNK (New England Biolabs, NEB) and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... followed by 5’-end phosphorylation with T4 PNK (New England Biolabs, NEB) and simultaneous blunt ligation of the plasmid using T4 DNA ligase (NEB) ...
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bioRxiv - Cell Biology 2020Quote: 5′ end phosphorylation and radiolabeling (1x PNK buffer, 40 U T4 PNK (NEB), 40 μCi 32P-γATP ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ phosphorylation and 3’ dephosphorylation were performed with T4 PNK (NEB, Cat: M0201S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: 5’ end phosphorylation (1x PNK buffer, 20 U T4 PNK (NEB, Cat# M0201L), 80 U RNaseOUT ...
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bioRxiv - Biochemistry 2024Quote: ... B2R phosphorylation site mutants mentioned in the manuscript were generated by site-directed mutagenesis using Q5 Site-directed mutagenesis kit (NEB, Cat. No. E0554). All DNA constructs used in this study were verified by sequencing from Macrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B, P6010, NEB) with or without 1 mM CX4549 (S2248 ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Cell Biology 2019Quote: In vitro phosphorylation reactions were carried out according to the manufactures instructions (NEB, P6010S) using 0.9 nanomoles of protein with control phosphorylation performed in the absence of ATP with all other reaction components ...
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bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
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bioRxiv - Cancer Biology 2022Quote: ... Phosphorylation of the 5’ end using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA, M0201L) (iii ...
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bioRxiv - Physiology 2022Quote: ... sense and anti-sense oligo DNAs (IDT) (Table S1) were phosphorylation using T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Eluted DNA was used to assemble phosphorylation-ligation reactions with 1X T4 DNA Ligase Buffer (NEB), 0.25 μL T4 PNK (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... The Oxiselect Comet Assay Kit (Cell-Biolabs) was used as a reference for the experiment ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by re-circularization through PNK phosphorylation of PCR product ends and ligation by T4 ligase (NEB). Similarly ...
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bioRxiv - Microbiology 2019Quote: ... and double-stranded spacer was generated by phosphorylation with T4 PNK (New England BioLabs, Ipswich, MA, USA) and annealed at 95°C for three minutes ...
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bioRxiv - Bioengineering 2021Quote: ... 5′ phosphorylation was performed either during synthesis (for tRNAfMet) or following synthesis using T4 Polynucleotide kinase (NEB)(for tRNALys and tRNAPhe ...
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bioRxiv - Bioengineering 2020Quote: ... Ligation and phosphorylation of PCR products were performed simultaneously using T4 DNA ligase (New England Biolabs, M0202L) and T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... New spacers were then cloned into the arrays by phosphorylation of oligo pairs with T4 PNK (NEB), hybridization of the oligo pair ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µL phosphorylation mix (1µL 100mM sense oligo, 1µL 100mM anti-sense oligo, 0.5µL 25mM ATP (BioLabs #P0756S), 1µL 10X PNK T4 Buffer (BioLabs #B0201S) ...
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bioRxiv - Cell Biology 2020Quote: ... Paired barcode and splints were phosphorylated in a PCR plate by combining 20 μl of oligonucleotides with 40 μl phosphorylation mix (1X T4 ligation buffer, 0.2 mg/ml BSA, 0.167 U/μl T4 PNK (NEB)) and incubated for 30 min at 37°C and heat inactivated for 20 min at 65°C ...
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bioRxiv - Microbiology 2019Quote: ... Each RNA was subjected to radioactive end-labeling or non-radioactive phosphorylation by T4 Polynucleotide Kinase (New England Biolabs). Each RNA was subjected to phosphorylation with equimolar concentrations of either 32P-γ-ATP or ATP ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 1 mM MnCl2 was used to remove the phosphorylation on the peptides and Staurosporin (NEB, Hitchin, UK) to occupy the active site pocket ...
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bioRxiv - Microbiology 2020Quote: ... the BioLux® Gaussia Luciferase Assay Kit (NEB, discontinued) or the GAR-2B Gaussia Luciferase Assay (Targeting Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’ end phosphorylation was performed by treating 10 μg total RNA with 0.5 μl of RNase inhibitor (20U final, NEB M0307L), 1 μl of 10x T4 PNK reaction buffer ...