Labshake search
Citations for New England Biolabs :
301 - 350 of 8886 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 89 bp segment surrounding the lesion site. PCR product was processed using T7 endonuclease assay (Fig. S6) per manufacturers instruction (NEB #E3321) to identify retwmr1/+ embryos which were then pooled and processed via previously described HCR and whole-mount immunohistochemical preparations ...
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bioRxiv - Cell Biology 2023Quote: ... Extracted genome DNA was used to amplify the surrounding region of the m.15059G>A mutation for T7 Endonuclease I (T7EI) mismatch detection assay using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, USA) in accordance with recent studies 27 ...
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bioRxiv - Neuroscience 2023Quote: PCR amplicons were tested for the presence of mutations using a T7 endonuclease I assay (New England Biolabs®, Ipswich, USA). 10 μL of each PCR was subjected to electrophoresis on a 1% agarose gel according to the protocol below ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB).
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB). The specificity of AlkB repair reaction was confirmed using an inactive AlkB control reaction in which Fe2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... or FLAG (amino acid sequence: DYKDDDDK) with a kit (Gibson assembly kit from New England Biolabs, catalogue # E5510S). The different combinations were cloned into pJFRC7-20XUAS-IVS-mCD8::GFP (Addgene # 26220 ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (kit number E7370L, New England Biolabs) and 8 cycles of PCR ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... NEB One Taq RT-PCR kit (One Taq® RT-PCR Kit, New England Biolabs INC, Frankfurt, Germany) was used for cDNA synthesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: NEBNext rRNA Depletion Kit (NEB, Cat E6310S) was used to deplete ribosomal RNA according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... The HiScribe Quick T7 kit (NEB, E2050S) was then used for in vitro transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... a quick ligation kit (New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB E0554S) and restriction cloning were used to generate partial constructs ...
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bioRxiv - Synthetic Biology 2019Quote: ... Q5 site directed mutagenesis kit from NEB was used for cloning the inserts inferiors at 100 bps ...
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bioRxiv - Microbiology 2019Quote: ... and end repaired (Quick Blunting Kit, NEB). Transposon junctions were amplified from gDNA by PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and Monarch Total RNA miniprep kit (NEB). RNA concentration was measured using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Phusion high fidelity DNA polymerase kit (NEB) was used for the PCR amplification and optimized with DMSO and formamide ...
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bioRxiv - Neuroscience 2019Quote: The Q5 Site-Directed Mutagenesis Kit (NEB) was used to create malt-1(C374A ...
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bioRxiv - Cell Biology 2019Quote: ... annealed and ligated (Quick Ligase kit, NEB) into BbsI (NEB ...
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bioRxiv - Genomics 2019Quote: ... blunt-end repair (Quick Blunting kit, NEB), 3’-adenylation (Klenow Fragment 3’ to 5’ exo- ...
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bioRxiv - Bioengineering 2019Quote: ... using the Gibson Assembly Cloning Kit (NEB), producing the plasmids pgRNAMPN372-142 and pgRNAMPN372-400 respectively ...
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bioRxiv - Bioengineering 2019Quote: ... using the Gibson Assembly Cloning Kit (NEB). The resulting plasmid pgRNA-triple-target is transformed in E ...
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bioRxiv - Physiology 2021Quote: ... and Monarch PCR Cleanup Kit (NEB, T1030). PCR products were electrophoresed in 2% agarose gel (TopVision Agarose Tablets ThermoFisher R2801 ...
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bioRxiv - Cancer Biology 2020Quote: ... using the Gibson Assembly kit (NEB#E2611). To verify the identity and relative representation of sgRNAs in the pooled plasmids ...
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bioRxiv - Cancer Biology 2020Quote: The Q5 Site-Directed Mutagenesis Kit (NEB) was used according to the manufacturer’s instructions to either delete the peak5 conserved 192 base pair sequence or mutate SoxE binding sites ...
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Barcoding and demultiplexing Oxford Nanopore native RNA sequencing reads with deep residual learningbioRxiv - Genomics 2019Quote: ... coli Poly(A) Polymerase kit (NEB-M0276S), following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... using Quick Ligation Kit (New England Biolabs). Positive pSpCas9(BB)-gRNA clones were confirmed by Sanger sequencing and expanded using PureLink® HiPure Plasmid Midiprep Kit (Invitrogen).
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bioRxiv - Synthetic Biology 2021Quote: ... and Monarch PCR & DNA Cleanup Kit (NEB) were used in this study for DNA purification.
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bioRxiv - Microbiology 2020Quote: ... using a Gibson Assembly Cloning Kit (NEB). Primers were designed to introduce a thrombin site between the P113 gene fragment and the coding sequence for Cd4-His6 on the P113-bio vector ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5 μl Murine RNase Inhibitor (NEB-kit) and 0.5 μl ProtoScript II RT (NEB-kit ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was capped Vaccinia capping kit (NEB) and recovered using Directzol RNA mini prep kit (Zymo research).
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bioRxiv - Cell Biology 2022Quote: ... using the Gibson Assembly Cloning Kit (NEB). HeLa cells were cotransfected using XtremeGENE 9™ (Roche ...
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bioRxiv - Microbiology 2022Quote: ... the NEB Ultra II FS kit (NEB #E6177L ...
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bioRxiv - Developmental Biology 2020Quote: ... using NEB Quick Ligation Kit (NEB, M2200S).
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... the Monarch PCR & DNA Cleanup Kit (NEB), or SPRI magnetic nucleic acid purification beads (UC Berkeley DNA Sequencing Facility) ...
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bioRxiv - Neuroscience 2020Quote: Illumina compatible libraries were made from Visium derived cDNA using a modified protocol derived from NEB Ultra II DNA FS kit (NEB #E6177). Visium derived spatial cDNA was diluted to 100ng in 26μl and combined with 7μl NEBNext Ultra II FS Reaction Buffer and 2μl NEBNext Ultra II FS Enzyme Mix and incubated at 37°C for 15min ...
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bioRxiv - Molecular Biology 2022Quote: ... or Q5-Site-directed mutagenesis kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... coli Poly(A) Polymerase kit (NEB-M0276S), following manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... using the NEBuilder kit (New England Biolabs). We submitted transgenic constructs to The BestGene Inc ...
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bioRxiv - Immunology 2020Quote: ... 1c using Gibson assembly kit (NEB, #E2611S). To promote the targeting efficiency ...
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bioRxiv - Microbiology 2020Quote: Libraries were mini-prepped (NEB Monarch Kit) and electroporated into the SEER chassis with more than 1,000-fold coverage ...
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bioRxiv - Molecular Biology 2021Quote: ... The Q5 mutagenesis kit (NEB, Cat: E0554) was used to generate the mutant plasmids ...
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bioRxiv - Bioengineering 2019Quote: ... A Q5 site-directed mutagenesis kit (NEB) was used to insert the Avitag DNA sequence into a pET15b plasmid containing the mutant polymerase gene ...
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bioRxiv - Genetics 2021Quote: ... a Gibson Assembly kit (New England Biolabs) was used to remove the myc-tag from Incenpmyc to generate IncenpWT-R ...
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bioRxiv - Plant Biology 2020Quote: ... 25 μL master mix (from NEB kit), and 1 μL each of a 25 μmol/L solution of each forward and reverse primer (forward 5′-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC* T-3′ ...
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bioRxiv - Immunology 2020Quote: ... using Gibson assembly kit (New England Biolabs) to create pcDNA3.1-mUNG2-polyGly-FLAG (mUNG2-FLAG ...
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bioRxiv - Microbiology 2021Quote: ... by Gibson Assembly Cloning Kit (NEB, #E5510S). HEK293T cells were transfected with the ISARL expression plasmid (pEZT-ISARL ...
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bioRxiv - Synthetic Biology 2021Quote: ... Monarch PCR & DNA Cleanup Kit (NEB #T1030L) was used to purify the fragments and they were all adjusted to the concentration of 100 nM to use for active learning experiments.