Labshake search
Citations for New England Biolabs :
451 - 500 of 10000+ citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: Genomic DNA was extracted using NEB Monarch HMW DNA extraction kit (T3060, New England Biolabs, France) according to manufacturer instructions with the following modifications ...
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bioRxiv - Genomics 2019Quote: ... Then the DNA on beads was used for PCR amplification with phusion DNA polymerase (NEB, M0530) and purified with VAHTS DNA Clean Beads to select the DNA fragments between 200bp and 600bp ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Genetics 2020Quote: ... RNA-DNA hybrids were ligated to Nanopore sequencing adapters (RMX) using T4 DNA ligase (NEB E6056) prior to sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Target genes were PCR amplified from genomic DNA using Phusion® High-Fidelity DNA Polymerase (NEB) and propagated following the instructions of CloneJET PCR Cloning Kit (Thermo) ...
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bioRxiv - Molecular Biology 2020Quote: DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) with index primers (NEB ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup ...
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bioRxiv - Microbiology 2020Quote: ... processed the extracted DNA with the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs, Ipswich, MA), and quantified the resulting DNA using Picogreen (Invitrogen).
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bioRxiv - Genomics 2021Quote: ... were amplified from TX1072 genomic DNA with Q5®High-Fidelity DNA Polymerase (New England Biolabs) and restriction sites for NdeI/XhoI and EcoRI/PmeI were included in the 5’ and 3’ primers (PK47 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples underwent methylated DNA depletion (MDD) using the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs), and the enriched fraction was used for DNA library construction as above (Figure 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng template DNA were mixed with 0.5 µl Phusion High-Fidelity DNA Polymerase (NEB, USA), 10 µl 5x GC Buffer (NEB ...
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bioRxiv - Molecular Biology 2021Quote: DNA constructs were built using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Cat. #E2621) and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat ...
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bioRxiv - Microbiology 2021Quote: ... DNA isolation from reaction mixtures was performed using Monarch PCR & DNA Clean-up Kit (NEB, USA). Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega ...
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bioRxiv - Neuroscience 2022Quote: DNA was purified using the Monarch PCR and DNA cleanup kit (New England Biolabs, 13-0041) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (NEB), with the insert size in the range of 150 to 250 bp ...
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bioRxiv - Biophysics 2022Quote: 10 kilobase long λ-DNA was prepared from full-length phage λ-DNA (New England BioLabs) by performing polymerase chain reaction (PCR ...
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bioRxiv - Developmental Biology 2022Quote: ... Ac5-DanFL was made by DNA assembly using NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S) from pAc5.1C-FLuc-V5His6 (Addgene plasmid #21183) ...
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bioRxiv - Molecular Biology 2022Quote: ... and genomic DNA was extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs). The thyA gene region was amplified from the genomic DNA (5 ng ...
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bioRxiv - Molecular Biology 2022Quote: ... and genomic DNAs were extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA libraries were constructed using the NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and sequenced using the Illumina HiSeq X Ten platform.
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bioRxiv - Molecular Biology 2022Quote: ... Eluted DNA was used to assemble phosphorylation-ligation reactions with 1X T4 DNA Ligase Buffer (NEB), 0.25 μL T4 PNK (NEB) ...
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bioRxiv - Genomics 2022Quote: ... were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB, #E6047A) in Quick Ligation Reaction Buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was extracted from cell pellets using Monarch Genomic DNA Purification Kits (New England Biolabs) and viral episome copies quantified by qPCR of the viral gene ORF57 as described in the qRT-PCR method.
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bioRxiv - Cell Biology 2023Quote: ... Tagrfp cDNA with flanking DNA segments encoding SGGGGS linkers was amplified using Phusion DNA polymerase (NEB) and inserted between Gly118- and Glu119-encoding codons of odr-3 (72 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we isolated high molecular weight DNA by following the Monarch T3010 DNA purification kit (NEB, USA) protocol ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... Template DNAs were prepared by polymerase chain reaction (PCR) using Q5 High-Fidelity DNA Polymerase (NEB) with the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... PCR amplification of purified DNA was done using Phusion High-Fidelity DNA Polymerase (New England Biolabs). The integrity of the purified raspberry DNA was demonstrated by amplification of a 325nt portion of the EIF 2.1 gene using primers 1468(F ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA purification was performed with the Monarch PCR & DNA Cleanup kit (New England Biolabs, Ipswich, MA), or the DNA Clean & Concentrator Kit (Zymo Research) ...
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bioRxiv - Microbiology 2023Quote: ... DNA assembly was done by using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). DNA digestion was performed with FastDigest restriction enzymes (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA was amplified for 30 cycles by adding 0.5ul Phusion High Fidelity DNA Polymerase (NEB; M0530S), 10ul of 5X Phusion HF Buffer ...
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bioRxiv - Genomics 2023Quote: ... 2.0 μg of the HMW DNA was treated with the NEBNext FFPE DNA Repair Mix (NEB) and the NEBNext UltraII End Repair/dA-Tailing Module (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... screening and validation of DNA sequences were performed using Taq DNA Polymerase (New England Biolabs, USA). T4 DNA ligase and restriction enzymes were purchased from New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from ear punch biopsies using the Monarch Genomic DNA purification kit (NEB). Transgenes were identified using PCR primers specific for each transgene (Suppl ...
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bioRxiv - Cancer Biology 2023Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted using the Monarch® Genomic DNA Purification Kit (New England Biolabs, Massachusetts; #T3010) with the Monarch® gDNA Tissue Lysis Buffer (#T3011 ...
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bioRxiv - Genomics 2023Quote: ... Homology arms were amplified from HaCat cell genomic DNA via PCR using Q5 DNA polymerase (NEB). The −loxP-PGK-Em7-NeoR-loxP- cassettes were amplified from PL452 (79) ...
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bioRxiv - Genomics 2023Quote: ... The hydroxy-methylated DNA fragment was combined with equal amounts of CpG methylated pUC19 DNA (NEB) and unmethylated lambda DNA (NEB) ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from each fraction using a Monarch Genomic DNA Purification Kit (NEB #T3010). Barcode sequences were amplified from gDNA using a two-step protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645L) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Sets 1 and 2 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Monarch® HMW DNA Extraction Kit for Tissue (New England Biolabs) and the supplied protocol for yeast ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was amplified by PCR using the Q5 High-Fidelity DNA polymerase kit (New England BioLabs) following the manufacturer’s recommendations and with primers designed around the mutations (supplementary Table S9) ...
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bioRxiv - Microbiology 2023Quote: ... and the DNA library preparation was performed using Q5 high-fidelity DNA polymerase (New England Biolabs). Peak-calling was performed as previously described [65].
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The deaminated ΦX174 DNA was purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Taq DNA Ligase (80 U, NEB), Phusion HF DNA Polymerase (0.5 U ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA contamination removed by DNaseI (NEB) treatment and reverse transcribed using M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... T4 DNA ligase (cat# M0202S, NEB) was used in the ligation reaction ...
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bioRxiv - Genetics 2021Quote: ... 10μl T4 DNA ligase (M0202L, NEB) and incubating at 16°C overnight ...
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bioRxiv - Genetics 2020Quote: ... using Taq DNA Polymerase (M0320S, NEB) and the following conditions ...