Labshake search
Citations for New England Biolabs :
401 - 450 of 10000+ citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Adapters were ligated to DNA using NEBNext Ultra DNA Library Prep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... For size comparison a radioactively labelled DNA ladder (100 bp DNA ladder, New England Biolabs) was applied.
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bioRxiv - Bioengineering 2023Quote: ... PCR was performed using synthesized primers (Integrated DNA Technologies) and Q5 DNA polymerase (NEB, M0491). The sequences of the constructed plasmids were verified by Sanger sequencing (Azenta) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was concentrated using the Monarch PCR and DNA Cleanup kit (New England Biolabs, MA) with warmed elution buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Genotyping PCR on purified genomic DNA was performed with Phusion High-Fidelity DNA Polymerase (NEB). PCR products were checked by agarose gel for single band amplification of the appropriate size and then subjected to Sanger sequencing for definitive identification of the 8 bp ch200 DNA deletion in ythdf2 exon 4 ...
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bioRxiv - Genetics 2023Quote: ... DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA from human cells was isolated using the Monarch®Genomic DNA Purification Kit (NEB) following the manufacturer’s instructions and including the recommended RNaseA digestion step ...
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bioRxiv - Biochemistry 2023Quote: All DNA constructs were cloned using the NEBuilder Hifi DNA Assembly System (NEB, cat # E2621X). All site directed mutagenesis reactions were performed using PrimeSTAR HS DNA Polymerase (Takara Bio ...
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bioRxiv - Genomics 2023Quote: ... and DNA was immediately isolated using the Monarch Genomic DNA Purification Kit (NEB, Cat # T3010S), with the modification that elution was carried out with 50 µL 25 mM K3BO3 solution (pH 7.0).
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bioRxiv - Genetics 2023Quote: DNA amplification was performed by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... DNA was purified using NEB Monarch PCR & DNA cleanup kit (New England Biolabs, catalog #T1030) according to the manufacturer’s instructions and eluted in 13µl H2O ...
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bioRxiv - Genetics 2023Quote: ... The immunoprecipitated DNA was purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs). The libraries for Illumina sequencing were constructed using the ThruPLEX DNA-Seq Kit (Clontech ...
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bioRxiv - Genomics 2023Quote: ... and DNA was immediately isolated using the Monarch Genomic DNA Purification Kit (NEB, Cat # T3010S), with the modification that elution was carried out with 87.5 μL 25 mM K3BO3 solution (pH 7.0).
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bioRxiv - Evolutionary Biology 2023Quote: ... NEBuilder HiFi DNA Assembly Master Mix and T4 DNA ligase from New England Biolabs (NEB). We obtained oligonucleotides from Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: Short molecular DNA was extracted using the Monarch Genomic DNA Purification kit (New England BioLabs). DNA quality and integrity were assessed with a Nanodrop and an Agilent 5400 ...
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bioRxiv - Genetics 2024Quote: ... DNA fragments were assembled using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs (NEB)) using the pGEM-T easy plasmid backbone ...
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bioRxiv - Genetics 2024Quote: ... DNA fragments were assembled using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs (NEB)) using the pGEM-T easy plasmid backbone ...
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bioRxiv - Genetics 2024Quote: ... DNA template with desired mutations and 3XP3EGFP cassette were assembled by HiFi DNA assembly (NEB). Wildtype esc donor constructs that also included the PAM site mutations were also generated for use as controls ...
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bioRxiv - Evolutionary Biology 2024Quote: ... extracted DNA was amplified using a two-stage Phusion High-fidelity DNA polymerase PCR (NEB). In a three-cycle PCR ...
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bioRxiv - Genomics 2024Quote: ... DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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bioRxiv - Genomics 2023Quote: ... resuspended with T4 DNA ligation mastermix (40 µl of T4 DNA Ligase Reaction buffer (NEB), 10% Triton X-100 ...
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bioRxiv - Plant Biology 2023Quote: ... DNA adaptator was ligated overnight with 2,000 u of T4 DNA ligase (New England Biolabs). Two PCRs were carried out by using nested primers designed on the adaptator and on the paromomycin resistance gene using the Advantage® GC genomic LA polymerase mix (Clontech) ...
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bioRxiv - Cancer Biology 2023Quote: ... Fragmented DNA samples was purified using the Monarch DNA Clean up Kit (New England Biolabs). The libraries were prepared from 5-10ng purified CUT&RUN-enriched DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C2566 genomic DNA was purified using Monarch Genomic DNA Purification Kit (NEB Ipswich, MA), GM12878 genomic DNA was obtained from Coriell Cell Repositories (Camden ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Neuroscience 2023Quote: ... we first generated the donor DNA with the N239K mutation using HiFi DNA assembly (NEB)(primers in Table S1) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA was then subjected to amplification using Q5 high-fidelity DNA polymerase (NEB) with a universal i5 primer and a uniquely barcoded i7 primer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three micrograms of DNA were repaired using the NEBNext FFPE DNA Repair Mix (NEB M6630). End repair and dA-tailing were performed using the NEBNext End repair/dA-tailing Module (E7546 ...
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bioRxiv - Microbiology 2020Quote: ... and the genomic DNA-transposon junctions were amplified (with Phusion® High-Fidelity DNA polymerase, NEB). Sequencing was performed using an Illumina MiSeq benchtop sequencer (Illumina ...
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bioRxiv - Genomics 2019Quote: ... were incorporated into DNA at nicks via nick translation with Taq DNA polymerase (New England Biolabs). A 10 μL reaction was prepared containing 1x ThermoPol buffer (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... The purified ligated DNA was subject to PCR amplification with Q5 high-fidelity DNA polymerase (NEB), followed by purification with Agencourt AMPure XP (Beckman Coulter) ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... Genomic DNA (gDNA) was extracted using the Monarch Genomic DNA Purification Kit (New England BioLabs, #T3010S) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... and used as template DNA for NGS sample preparation using Q5 High-Fidelity DNA Polymerase (NEB) (primers shown in Supplementary Table S3) ...
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bioRxiv - Molecular Biology 2019Quote: ... Associated DNA was then purified by extraction using Monarch PCR & DNA Cleanup Kit (New England Biolabs). Immunoprecipitated DNA and total input were used as a template for real-time qPCR ...
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bioRxiv - Plant Biology 2019Quote: ... the respective promoter regions were amplified from genomic DNA using Phusion DNA Polymerase (New England Biolabs) and promoter-specific primers (Table S1 ...
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bioRxiv - Neuroscience 2019Quote: ... DNA libraries were constructed using NEBNext UltraII DNA Library Prep Kit for Illumina (New England BioLabs) and quantified using KAPA Library Quantification Kit for Illumina (KAPA Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... DNA from the excised fragments was extracted using the Monarch® DNA Gel Extraction Kit (NEB). Before cloning ...
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bioRxiv - Microbiology 2021Quote: ... 600□units of T4 DNA ligase and 75□units of T7 DNA polymerase (New England BioLabs) to prime in vitro DNA synthesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA templates for MS-QBiC peptide synthesis were amplified by PCR using Taq DNA polymerase (NEB) with a T7 promoter primer (5′-GGGCCTAATACGACTCACTATAG-3′ ...
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bioRxiv - Plant Biology 2020Quote: ... The DNA library was made using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB), size selected for inserts of 500 to 600 bp ...
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bioRxiv - Microbiology 2020Quote: ... Restriction DNA enzymes and T4 DNA ligase were purchased from England Biolabs (NEB, Ipswich MA, USA) or Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid-Safe DNase-treated DNA was amplified using phi29 DNA polymerase (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and DNA libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genomics 2022Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 0.02 U Taq high fidelity DNA-polymerase (Q5 High-Fidelity DNA Polymerase, New England Biolabs). We used a set of long primers developed to have a 5’ flanking sequence complementary to the Nextera XT DNA index to facilitate adapter ligation during library construction:
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bioRxiv - Microbiology 2021Quote: ... DNA libraries were generated using a NEBNext UltraTM II DNA Library Prep Kit (NEB, Ipswich, USA) and were then sequenced on an Illumina NovaSeq 6000 platform (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Sheared DNA was purified using either the Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA) or QIAquick Nucleotide Removal Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2020Quote: ... All DNA fragments were purified from agarose gel (Monarch DNA Gel Extraction kit, New England Biolabs) to reduce carry-over of residual contaminants.