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Citations for New England Biolabs :
301 - 350 of 917 citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... rinsed with PBS and incubated in PBS containing 3% molecular biology grade BSA (New England Biolabs) and 0.05% Tween-20 for 1 h at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Phages containing the library were rescued using M13KO7 Helper Phage (New England Biolabs, Ipswich, MA, USA). Solution-phase bio-panning was conducted in order to minimize the disruption of a target protein ...
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bioRxiv - Biochemistry 2021Quote: ... media was replaced with media containing 3 µM SNAP-Cell block (New England BioLabs cat. # S9106S) and cells maintained at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Biophysics 2022Quote: ... containing TwinStrep-SUMO at the N-terminus of S15 using the NEBuilder HiFi assembly protocol (NEB). TnsC clones were transformed into Escherichia coli BL21(DE3 ...
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bioRxiv - Genomics 2022Quote: ... the 1μl DNA was mixed with 22μl reaction buffer containing 1x phi29 reaction buffer (NEB, M0269S), 20μM random primers (Thermo ...
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bioRxiv - Genomics 2023Quote: ... the DNA was treated in a 30 µL reaction containing 12 U T4 polynucleotide kinase (NEB), 1 mM ATP (NEB) ...
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bioRxiv - Genomics 2023Quote: ... The region containing the linker-mAID sequence was then amplified by PCR using Phusion polymerase (NEB) from the modified pMK287-mAID-Hygro plasmid and subcloned into the pL452 vector which harbors the Neomycin resistance cassette under the control of a PGK promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Biochemistry 2023Quote: Plasmids containing the α and β genes were freshly transformed in E.Coli BL21-DE3 cells (NEB). Transformed cells were plated on LB agar plates containing 100ug/ml Carbenicillin and grown overnight at 370C overnight ...
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bioRxiv - Microbiology 2023Quote: ... the closed 215-bp virS-containing DNA fragment was open by cleavage with NdeI (NEB, Germany). To this end ...
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bioRxiv - Plant Biology 2023Quote: ... the pMIR319C containing pMW#2 reporter constructs were linearized with XhoI (R0146, New England Biolabs, USA) and integrated into the mutant HIS3 locus of the YM4271 host strain (Gift from Ram Yadav ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Bioengineering 2023Quote: ... containing 10 μL 2X Luna® Universal Probe qPCR Master Mix (M3004, New England Biolabs, USA) (final concentration 1X) ...
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bioRxiv - Molecular Biology 2023Quote: ... the plasmid containing the origin DNA sequence was digested with HindIII and EcoRI (New England Biolabs) according to manufacturer’s protocol (Supplemental Figure S2A ...
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bioRxiv - Molecular Biology 2023Quote: ... KUNV-wt and their reporter-containing variants were linearized using AgeI-HF enzyme (NEB, United States) prior in vitro transcription ...
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bioRxiv - Molecular Biology 2023Quote: Endpoint PCR was performed in a 20 µL reaction volume containing 1× OneTaq Master Mix (NEB), 0.2 µM of each primer and 1 µL of 100-fold diluted cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Cancer Biology 2023Quote: ... we prepared a digestion mix containing 100 nL of NlaIII restriction enzyme (NEB, cat. no. R0125L) and 50 nL of CutSmart buffer (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: The plasmid library containing static barcodes was generated by transforming high-efficiency competent cells (NEB C3019), then plating them onto a large surface area of LB-agar (∼30 10-cm petri dishes ...
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bioRxiv - Neuroscience 2024Quote: 10µg of a plasmid containing 807bp of Pcdh10 exon 1 sequence was linearized with SacII (NEB) in 1x CutSmart buffer (NEB) ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201] ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-butyryllysine antibody (PTM BioLabs, PTM#301) at 1:2000 dilution was incubated with the membrane overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using a pre-poured amylose column containing 4 mL amylose resin (New England Biolabs, E8021L) followed by size exclusion chromatography (protein buffer ...
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... Derivatives of pEAI423 containing mutations in EXO1 were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAI423 as template and the oligonucleotides shown in Table S7 ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated in 100 μl end-repair mix containing 1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Biophysics 2022Quote: ... CPB containing a C-terminal His6-tag was generated using Q5® site-directed mutagenesis kit (NEB), pET-19b_His6_CPB as template and primer pair #1 and elongation time of 4 min to delete the N-terminal His6 ...
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bioRxiv - Physiology 2020Quote: ... 1 nl of CRISPR mixture containing 2,4 μg/μl of gRNA and 0.5 μl Cas9 protein (NEB) was injected in one-cell stage embryos and raised for 24 hours ...
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bioRxiv - Microbiology 2019Quote: ... with the reaction containing 5mM m7G(5′)ppp(5′)G RNA Cap Structure Analog (New England BioLabs). Resulting RNA was purified by lithium chloride precipitation and transfected into BHK-21 cells using Lipofectamine 3000 for viral production ...
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bioRxiv - Microbiology 2019Quote: ... with the reaction containing 5mM m7G(5′)ppp(5′)G RNA Cap Structure Analog (New England BioLabs). Resulting RNA was purified by lithium chloride precipitation ...
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bioRxiv - Genetics 2019Quote: ... The protospacer-containing plasmids were further modified by excising the Cas9 ORF with PstI (New England Biolabs). Each final plasmid contains the U6 promoter ...
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bioRxiv - Plant Biology 2021Quote: ... 2019) was replaced by the coding sequences containing the phosphosite mutations using Gibson Assembly (New England Biolabs). To create transformation vectors NPH3::GFP-NPH3-R18 and NPH3::GFP-NPH3-mR18 ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... and ligated with oligos each containing a Cy5- or BHQ-2 label and NotI sticky ends (NEB) complimentary to the amplified DNA template ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated in a final volume of 40μL containing 1X G5 reaction buffer (New England BioLabs) and incubated at 37°C for 4 hrs in the absence (mock ...
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bioRxiv - Molecular Biology 2022Quote: ... the modifications included employment of deoxy uridine containing primers and USER enzyme mix (New England Biolabs, UK) to create sticky ends that would facilitate the circularization of plasmid [9] ...
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bioRxiv - Microbiology 2022Quote: ... Parallel T7 reactions performed at the same time used 1.25 μg XbaI linearised pT7HCV(5’+)341N79wt and pT7HCV(5’+)341N79ko as templates in a 25μl final reaction volume containing 2.5μl 10 x T7 transcription buffer (NEB), 0.6μl RNaseOUT ...
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bioRxiv - Immunology 2020Quote: ... the pSS013-Cas9 vector containing a sgRNA against GRA24 was transfected along with NotI (New England Biolabs) linearized pLoxP-DHFR-mCherry-LoxP (Addgene Plasmid #70147) ...
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bioRxiv - Molecular Biology 2019Quote: ... the integrative plasmid pJRL2-pBMH1-yEGFP containing homologies to LEU2 was cut by AscI (New England Biolabs) and transformed ...
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bioRxiv - Biochemistry 2019Quote: ... Washed beads were incubated with 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) in a head-over-head rotator overnight at 37 °C.
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bioRxiv - Developmental Biology 2019Quote: ... E7.5) into 96 well PCR plates containing 2.5μl of methylase reaction buffer (1× M.CviPI Reaction buffer (NEB), 2U M.CviPI (NEB) ...
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bioRxiv - Immunology 2021Quote: ... the scFv containing region from isolated plasmids was amplified by PCR using Q5 high fidelity PCR (NEB). Illumina NGS samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols pooling four library samples per lane ...
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bioRxiv - Genomics 2022Quote: ... was used in a 50 μl-reaction containing 5 μl of NEBuffer r3.1 (10X, NEB, cat# B7203), 26 μl of adapter-ligated DNA and NF water to 50 μl ...
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bioRxiv - Molecular Biology 2022Quote: ... containing hydrophilic streptavidin magnetic beads (equalling to 260 μl of the original bead suspension; New England Biolabs), pre-equilibrated with 1× Annealing Buffer ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Microbiology 2022Quote: pJJW101 derivatives containing one of the four NT ospA-targeting sgRNAs were cut with BglI (NEB, R0143), dephosphorylated with Quick-CIP (NEB ...