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Citations for New England Biolabs :
4851 - 4900 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: UASp-KibS677A-GFP-FLAG was generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, Catalog #E0554S) using primers KibS677A (see primers table) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA was used for cDNA synthesis using the ProtoScript® II kit (New England BioLabs). qPCR reactions were carried out with FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (
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bioRxiv - Microbiology 2021Quote: ... and linker region were inserted into the relevant constructs also by site-directed mutagenesis (NEB Q5-based kit). Optimized DNA sequence is provided in figshare File 1 (10.6084/m9.figshare.c.5390285) ...
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bioRxiv - Microbiology 2021Quote: ... and barcoded using the NEBnext Ultra-directional RNA Library Kit for Illumina (New England Biolabs, Ipswitch, MA, USA) with NEBnext Multiplex Oligos for Illumina (Primer Set 1 ...
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bioRxiv - Microbiology 2021Quote: ... The DNA concentration was determined with the NEBNext Library Quant Kit for Illumina (New England Biolabs, MA, USA) and pooled in equimolar ratios with unique barcodes for each sample ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was purified from cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs, USA). Purified DNA samples were eluted in 30µl nuclease-free water and quantified on a Nanodrop One (Thermo Fischer Scientific ...
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bioRxiv - Zoology 2020Quote: ... Sequencing libraries were generated using NEBNext®Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2020Quote: ... Fragments for assembly were purified using agarose electrophoresis and gel extraction (Monarch DNA Gel Extraction Kit, NEB T1020L). For assembly ...
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bioRxiv - Biochemistry 2021Quote: ... Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB), and the resulting library was sequenced using Illumina MiSeq v2 (2×150 bp ...
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bioRxiv - Cancer Biology 2019Quote: ... In vitro transcription was carried out by the HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs). Resulting transcriptions products were treated with DNAse I ...
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bioRxiv - Microbiology 2021Quote: ... sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S).
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bioRxiv - Bioengineering 2020Quote: ... The RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s instructions [51] ...
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bioRxiv - Developmental Biology 2020Quote: ... Stranded cDNA libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primer Set I ...
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bioRxiv - Immunology 2020Quote: ... Illumina adaptors were added using the NEBNext® Ultra™ II DNA Library Prep Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Site-directed mutagenesis was performed using the NEB Q5 site-directed mutagenesis kit (NEB, Ipswitch, MA, cat #E0554S) using mutagenesis primers summarized in Table S2 with pBADmobshyA as a template.
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bioRxiv - Zoology 2019Quote: ... DNA libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA), and NEBNext Multiplex Oligos for Illumina Index kit (New England BioLabs ...
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bioRxiv - Cancer Biology 2019Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Libraries were sequenced using SE 75 bp Illumina NextSeq.
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bioRxiv - Developmental Biology 2019Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... digested pCFD6 backbone using the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Cat. No: R5520S), following the instructions provided by the manufacturers.
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bioRxiv - Genetics 2020Quote: ... pyogenes chimeric single guide RNA through in vitro transcription using a HiScribe T7 Quick kit (New England Biolabs). The gRNA was then precipitated in a 1/10 volume of 3M Sodium Acetate and two volumes of 100% ethanol by chilling the reaction at −20 °C for 15 min ...
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bioRxiv - Genetics 2020Quote: ... The small RNA fraction was used with the NEBNext small RNA library prep kit (New England Biolabs, #E7300) to produce a small RNA library ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... We then created a construct containing the alternate allele using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were:
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bioRxiv - Genetics 2020Quote: ... The purified dephosphorylated linear plasmid and phosphorylated annealed oligos were ligated together using the Quick Ligation Kit (NEB). The ligated product was transformed into One Shot Stbl3 Chemically Competent E ...
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bioRxiv - Genetics 2020Quote: ... TrueSeq-barcoded RNA-seq libraries were generated with the NEBNext Small RNA Library Prep Kit (New England Biolabs) and size selected for insert sizes ∼18-50bp ...
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bioRxiv - Genetics 2020Quote: mRNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) as described (29) ...
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bioRxiv - Microbiology 2021Quote: ... libraries were prepared using NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IgG /IgK/IgL NGS libraries were made by using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on the Illumina Miseq 2 × 300 platform ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA libraries were generated according to NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760). cDNA was amplified for 12 cycles and barcoded with NEBNext Multiplex Oligos for Illumina (NEB E7600) ...
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bioRxiv - Genomics 2021Quote: The whole genome bisulfite (WGBS) libraries were prepared using the Ultra II DNA library prep kit (NEB E7645) before being Bisulfite converted using the Zymo EZ DNA Methylation-Gold bisulfite conversion kit according to the protocol described in Vaisvila et al ...
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bioRxiv - Immunology 2020Quote: ... and the quantity of the library was determined by qPCR using NEBNext Library Quant Kit for Illumina (NEB) according to manufacture’s instruction ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... Pre-adenylated DNA linker(DNA oligo purchase from IDT was adenylated using 5′ DNA Adenylation Kit (NEB,E2610S)) was ligated to the 3’-end of RNA in a 20 µl reaction (25% PEG8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA libraries were made using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs # E7645S) according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2020Quote: ... The RT-PCR detection was conducted by using the Luna Universal One-Step RT-qPCR kit from NEB and following its protocol ...
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bioRxiv - Genomics 2019Quote: ... Paired-end sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced on the Illumina NovaSeq 6000 Sequencing System with unique dual indexes across multiple flowcells at 2×51 base pairs.
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bioRxiv - Developmental Biology 2021Quote: ... This fragment was inserted in front of a codon optimized GFP via the HiFi DNA Assembly kit (NEB) with the addition of a membrane-tethering CAAX domain at the C-terminus to visualize the morphology of the cells expressing the reporter protein ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England BioLabs, Inc., USA) using primer pairs P11/P12 (G63S) ...
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bioRxiv - Biophysics 2021Quote: ... The Vimentin-HALO construct was obtained after cloning using the HiFi DNA Assembly Cloning Kit (Biolabs cat # E5520S) starting from the tubulin-HALO plasmid (addgene #64691 ...
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bioRxiv - Biochemistry 2021Quote: ... also C-terminal) FT MYOC variants were generated by DNA assembly (NEBuilder HiFi DNA Assembly Cloning Kit, NEB) by combining a PCR-amplified eGLuc2 product from a pcDNA3 MYOC eGLuc2 plasmid (originally described previously8) ...
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bioRxiv - Biochemistry 2021Quote: ... metoecus Cmr interference complex (VmeCmr) was assembled using the NEBuilder® HiFi DNA Assembly kit (New England Biolabs) following the Gibson assembly strategy (43) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 μg of DNA library was used as a template for mRNA synthesis by HiScribe T7 kit (NEB). The product was purified by NH4Ac precipitation and dissolved in RNAse-free water to a final concentration of 3 μg/ul.
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bioRxiv - Genomics 2020Quote: ... Protocols and reagents from NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (New England Biolabs) were used for end repair ...
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bioRxiv - Bioengineering 2020Quote: ... RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Neuroscience 2020Quote: ... Truncated Hsp70 constructs were made using a Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then constructed with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB) following the manufacturer’s instructions and sequenced on an Illumina paired-end platform with 150 bp per read length by Annoroad Gene Technology Co. ...
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bioRxiv - Developmental Biology 2021Quote: ... ChIP-seq libraries were prepared using the NEB Next Ultra II DNA Library Prep kit (New England Biolabs) following the manufacturer’s instruction and subjected to high-throughput sequencing on an Illumina HiSeq 2500 system (150 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently in-vitro transcribed using HiScribe™ T7 Quick High Yield RNA Synthesis Kit from NEB (#E2050S). The IRE-1 RNase-specific inhibitor 4μ8c was used at 5 μM.