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Citations for New England Biolabs :
4801 - 4850 of 10000+ citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... The NebNext Ultra II RNA library prep kit (New England Biolabs, MA, USA) was used to synthesize RNA libraries ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR products were ligated using a Gibson assembly kit (NEB, USA). The resulting plasmid pLZ asnA R100K was electroporated into strain S119 ΔasnA for expression of mutated asnA.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NEB Monarch RNA prep kit (New England BioLabs). The RNA concentration and RIN (RNA integrity number ...
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bioRxiv - Genomics 2024Quote: ... and used for Illumina library using the NEBNext ultra II kit (NEB E7600). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were purified using a Monarch PCR Cleanup Kit (New England Biolabs) and prepared for nanopore long read sequencing using the Ligation Sequencing Kit (Oxford Nanopore Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... PCR products were purified using Monarch DNA Gel Extraction Kit (New England Biolabs). Both the vector and the complete insert were digested by BsmBI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... before purifying again using an NEB RNA clean and concentrate kit (NEB #T2030).
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) was used for the following RNA-seq library preparation according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... PCR products were purified using a Monarch PCR & DNA Cleanup Kit (NEB, T1030S) following the Manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB). The first-dimension electrophoresis was run in 0.7% agarose in 1xTBE buffer at 0.7 V/cm for 18h ...
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bioRxiv - Genomics 2024Quote: ... gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the Manufacturer’s protocol for cultured cells with an input of 5e6 HeLa cells ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) library preparation kit ...
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bioRxiv - Developmental Biology 2024Quote: ... They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L) and oligo(dT ...
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bioRxiv - Molecular Biology 2024Quote: ... For library preparation NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using Ultra II FS library preparation kit (New England Biolabs) and amplified with KAPA HiFi HotStart Real-time PCR using KAPA P5 and P7 primers (KAPA Biosystems).
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bioRxiv - Molecular Biology 2024Quote: ... m6A-RIP mRNA was purified using the Monarch RNA Cleanup Kit (NEB, T2030S). Samples were eluted in 8 µl elution buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit v2 (NEB; E7400L) and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Quick change was performed using Q5® Site-Directed Mutagenesis Kit (NEB: E0554S) to mutate the lysines at position 26-27 to glutamines or alanines ...
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bioRxiv - Neuroscience 2024Quote: ... The promoter was cloned using the Gibson Cloning Assembly Kit (New England BioLabs) following standard procedures ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using the NEBNext® Enzymatic Methyl-seq Kit (NEB, #M7634) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Phosphorylated samples were ligated using a T4 DNA ligase kit (New England Biolabs) and incubated overnight at 16°C ...
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bioRxiv - Plant Biology 2024Quote: ... and SMXLχ121077ΔRGKT were generated with Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Inserts in Gateway entry clones were transferred into pGWBcitr-SMAX1pro and pGWBcitr-SMXL7pro by Gateway LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... All fragments were gel-purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the full-length library insert ...
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bioRxiv - Biochemistry 2024Quote: ... Mutagenesis was performed using the Q5 site-directed mutagenesis kit (NEB, Cat#E0552S) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Site-directed mutagenesis was performed by the Q5 site-directed mutagenesis kit (NEB) with primers designed to harbor the mutation site(s) ...
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bioRxiv - Biochemistry 2024Quote: ... An Illumina-compatible library was prepared with NEB Ultra II DNA kit (NEB) and each library was shotgun sequenced with Illumina HiSeq3000 (2 x 150 bp ...
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bioRxiv - Neuroscience 2024Quote: Constructs were all generated using the Gibson Assembly Cloning Kit (New England Biolabs) by inserting the different DNA fragments (PCR-generated or gBlocks ...
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bioRxiv - Cancer Biology 2024Quote: ... library preparation (NEBNext Ultra II RNA Library Prep kit, New England Biolabs Inc.) and Illumina NovaSeq 2 × 150 bp paired-end sequencing ...
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bioRxiv - Biochemistry 2024Quote: ... The total RNA was reverse transcribed using the LunaScript RT SuperMix Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesised using the LunaScript RT SuperMix Kit (New England Biolabs, E3010L) as per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Selections were then purified using the Monarch® PCR & DNA Cleanup Kit (NEB), eluting in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs). RNA quality was confirmed after each purification with a TapeStation 4150 (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were extracted from each strain by miniprep kit (New England Biolabs, T1010L) and whole plasmid sequencing was performed by Plasmidsaurus using Oxford Nanopore Technology with custom analysis and annotation ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB) and checked on 1% agarose gel and approximately 600 ng of each PCR product was used as template for in vitro dsRNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was then purified using the Monarch PCR & DNA Cleanup Kit (NEB), and RNA was synthesised from the purified DNA using the MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was depleted using the bacteria rRNA depletion kit (New England Biolabs), with the addition of human rRNA depletion beads for the samples containing S ...
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bioRxiv - Genomics 2024Quote: ... Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Developmental Biology 2024Quote: ... and quantified using a NEBNext® Library Quant Kit for Illumina® (NEB) on a QuantStudioTM 5 Real-Time PCR System (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: ... followed by purification with a Monarch® PCR & DNA Cleanup Kit (NEB®). Standard experimental conditions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the Monarch PCR and DNA Cleanup Kit (New England Biolabs). 200 ng of purified product was used in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific], 38 μM dCTP, dGTP and dCTP [Thermo Fisher Scientific], 50 U Klenow Polymerase [NEB], 1x NEB 2 buffer) and cells incubated for 1 h at 37 °C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were spun down and resuspended in fill-in mix (biotin-14-dATP [Thermo Fisher Scientific], dCTP, dGTP and dTTP [Thermo Fisher Scientific], Klenow Polymerase [NEB], 1x NEB 2 buffer) for 1.5 h at 37°C with rotation ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10 µg of vector and 1 µg of amplicon were digested overnight with 2 µl (vector) or 0.5 µl (amplicon) each of SphI-HF® and NsiI-HF® (New England Biolabs, catalog no. R3127) in a total volume of 50 µl ...
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Cis-regulatory divergence underpins the evolution of C3-C4 intermediate photosynthesis in MoricandiabioRxiv - Plant Biology 2021Quote: ... 17 μl total RNA (100 ng/μl) was added with 2 μl buffer and 0.5 μl RNase-free DNaseI enzyme (New England Biolabs GmbH, Frankfurt am Main, Germany) incubating on ice for 30 s ...
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bioRxiv - Molecular Biology 2022Quote: ... Beads were washed as described above and either directly resuspended in Proteinase K reaction or in 20 μl of RecJ adapter removal reaction (1X NEB Buffer 2 (NEB, #B7002S), 25U 5’ Deadenylase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA templates for transcription were obtained by PCR amplification of the psiCheck-2 vectors using a forward primer containing an SP6 promoter sequence by Phusion HF polymerase (NEB, USA, Cat. No. M0530S). After transcription ...
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bioRxiv - Genomics 2022Quote: ... and the presence or absence of one of two RNAse inhibitors (1 – Millipore Sigma, Protector RNAse Inhibitor, Cat. No. 3335399001; 2 – NEB, RNAse Inhibitor, M0314S). The RNA was then extracted from the tissue using the miRNeasy kit (Qiagen Cat ...
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bioRxiv - Plant Biology 2022Quote: ... 250 μL 2× lysis buffer (20 mM Tris-HCl, 40 mM Na2EDTA, 1% (w/v) SDS) and 3 μL proteinase K (NEB: P8102S, 20 mg/mL) were added and incubated under agitation at 55°C for 2 h ...
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bioRxiv - Genomics 2020Quote: ... Between 100 and 200 ng of genomic DNA was digested using 2 units of the MslI restriction enzyme (New England Biolabs ®, Ipswich, Massachusetts, USA.) and using 1 unit of NEB4 buffer in 20 μl of volume and for 2 hours at 37 °C ...