Labshake search
Citations for New England Biolabs :
4751 - 4800 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Mutations were introduced into RARα-encoding plasmids using Q5 Site-Directed Mutagenesis Kit (NEB E0554). Mutations were confirmed by sequencing of the resulting plasmids.
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bioRxiv - Biochemistry 2023Quote: ... PCR purifications with the Monarch® PCR & DNA cleanup kit (New England Biolabs, Ipswich, USA), extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs Inc., USA) as described by the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplicons were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... and DNA was immediately isolated using the Monarch Genomic DNA Purification Kit (NEB, Cat # T3010S), with the modification that elution was carried out with 50 µL 25 mM K3BO3 solution (pH 7.0).
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bioRxiv - Microbiology 2023Quote: ... in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765). To facilitate the design of deer-specific ssDNA probes Custom RNA Depletion primers were designed using the tool https://depletiondesign.neb.com/ ...
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bioRxiv - Microbiology 2023Quote: ... Oligomerization mutant LANA was created by Q5 site directed mutagenesis kit (cat no# NEB E0554S). For ChIP assays we cloned the dCas9-5xSunTag from pCAG-dCas9-5xPlat2AflD ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and star-nose mole by using a Monarch Total RNA Miniprep Kit (New England BioLabs), with DNase I on column digestion to remove genomic DNA contamination ...
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bioRxiv - Cell Biology 2023Quote: ... using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, USA) and a BIORAD CFX Opus 384 system ...
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bioRxiv - Microbiology 2023Quote: ... the drop-out plasmid pHC8 was generated by Golden Gate Assembly kit (New England BioLabs), and the basic genetic parts are from Marburg collection (65) ...
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bioRxiv - Immunology 2023Quote: ... DNA was purified using NEB Monarch PCR & DNA cleanup kit (New England Biolabs, catalog #T1030) according to the manufacturer’s instructions and eluted in 13µl H2O ...
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bioRxiv - Microbiology 2023Quote: ... and pEJK29 (YopRY304A) using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs; Ipswich, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the amplified oligonucleotide pool was gel-purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the linearized vector for the light chain variant library ...
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bioRxiv - Genetics 2023Quote: ... The following sequences were assembled using the Gibson Assembly cloning kit (New England Biolabs E5510S): mCherry2-N1 amplicon (a gift from Michael Davidson ...
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bioRxiv - Immunology 2023Quote: ... and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB). After DNase treatment ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB) ...
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bioRxiv - Physiology 2023Quote: ... and NEBNext Ultra RNA Library Prep Kit for Illumina (cat. E7530) were purchased from NEB. AMPure XP Beads (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and Multiplex Oligos for Illumina Systems (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons (approximately 3.4 kb) were gel purified using Monarch DNA gel extraction kit (NEB). Library construction ...
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bioRxiv - Cell Biology 2024Quote: ... The R36K variant was generated with the Q5® Site-Directed Mutagenesis Kit (NEB, # E0554S) from the pERLIN2-3xFlag using the manufacturer’s protocol (primers sequence ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the NEBNext® UltraTM II Library Prep Kit (New England Biolabs). Paired-end 150 sequencing was performed using the NextSeq 500 platform (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and analyzed with either 2100 Bioanalyzer or TapeStation systems (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: All constructs were cloned using the NEBuilder HiFi DNA Assembly kit (New England Biolabs E2621) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the Directional RNA Library Prep Kit (E7760S, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the Monarch® HMW DNA Extraction kit for Tissue (New England Biolabs, Ipswich, MA, USA) was used to improve DNA fragment length ...
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB), and sequencing was performed on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was done with 100ng of RNA with the Luna RT-qPCR kit (NEB) using primers targeting beta actin as a control (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then prepared for sequencing with the NEBNext Small RNA library Prep kit (NEB). Sequencing libraries were visualized using an Agilent 2200 Tapestation and sequencing was done using the Novaseq 6000 SP300 cartridge (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, E7420) and amplified by PCR with KAPA HiFi HotStart Ready Mix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were performed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the published protocol50 ...
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bioRxiv - Molecular Biology 2023Quote: HLTF’s cDNA on the pcDNA3.1(+) backbone54 was mutated using Q5 Site-Directed Mutagenesis kit (NEB) to generate the C760S and R890Q mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... using the High Sensitivity DNA Assay and NEBNext Library Quant Kit for Illumina (NEB, E7630). Finally ...
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bioRxiv - Genomics 2023Quote: ... and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The mSA S27A mutation was created by site-directed mutagenesis using commercially-available kits (NEB). 4NBX.B_C103 and 2X89.A_CCAA_C57 sequences were directly ordered from IDT ...
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bioRxiv - Genetics 2023Quote: RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The FBXO22-C326A mutant plasmids are generated via Q5 Site-Directed Mutagenesis Kit (NEB E0552) based on the wildtype FBXO22-HaloTag plasmid.
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using the Monarch® Total RNA Miniprep Kit (#T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was generated using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs). SARS-CoV-2 was detected by PCR using the 2019-nCoV RUO Kit (IDTDNA ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on mEGFP-ATP7B were prepared following Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L) was used for library preparation and libraries were sequenced on Illumina MiSeq or NextSeq 2000 platform and more than 1.25M read pairs of 150bp length were generated per sample i.e ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a NEBNext Ultra II DNA library prep kit (NEB, Cat# E7645S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...