Labshake search
Citations for New England Biolabs :
4651 - 4700 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Plasmid DNA were extracted from bacterial cultures using Monarch Plasmid Miniprep Kit (New England Biolabs) and sequenced using the BigDye Terminator v.1.1 kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and analyzed with either 2100 Bioanalyzer or TapeStation systems (Agilent) ...
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bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... the K129A mutation was introduced using the Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... mutations (Figure 5I) were introduced using the Q5 site-directed mutagenesis kit (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and underwent paired end sequencing in a NextSeq500 or HiSeq PE150 (Novogene).
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB). After library QC analysis using Bioanalyzer High Sensitivity DNA kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The six pieces were assembled with the Golden Gate BsaI-HF v2 assembly kit (NEB). Three guide RNA (gRNA ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
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bioRxiv - Microbiology 2023Quote: ... Gibson cloning was performed according to the protocol of the Gibson Assembly kit (NEB, E5520). To construct the Sch9-3E-NAT mutant and the matching Sch9-WT-NAT control strains ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting Fluc mRNA was purified twice by Monarch RNA cleanup kit (T2040S, NEB, Ipwsich, MA) to ensure that unincorporated nucleotides were completely removed ...
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bioRxiv - Immunology 2023Quote: ... and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB, Cat.#E7103S). Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs) or by Qubit (Thermo) ...
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bioRxiv - Plant Biology 2024Quote: ... The NEB Next® DNA Library Prep Master Mix set for Illumina kit (NEB #E6040S) was implemented to prepare the DAP-Seq gDNA library ...
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bioRxiv - Cell Biology 2024Quote: ... Serine-35 mutations were constructed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers are listed in the supplemental methods.
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: The sequencing library was constructed using the NEBNext Ultra II FS Kit (New England Biolabs) according to the prime-seq protocol ...
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bioRxiv - Immunology 2024Quote: ... and PCR products were assembled using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) to generate the final construct pET788 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reaction mix was purified with Monarch® PCR & DNA Cleanup Kit (NEB, Ipswich, MA). Next ...
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bioRxiv - Evolutionary Biology 2024Quote: Short molecular DNA was extracted using the Monarch Genomic DNA Purification kit (New England BioLabs). DNA quality and integrity were assessed with a Nanodrop and an Agilent 5400 ...
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bioRxiv - Genetics 2024Quote: ... DNA fragments were assembled using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs (NEB)) using the pGEM-T easy plasmid backbone ...
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bioRxiv - Genetics 2024Quote: ... DNA fragments were assembled using the NEBuilder HiFi DNA Assembly Kit (New England Biolabs (NEB)) using the pGEM-T easy plasmid backbone ...
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bioRxiv - Genetics 2024Quote: ... Next-generation sequencing libraries were prepared using NEBNext Multiplex Small RNA Library Prep Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... a SYBR green I based qRT-PCR kit (Luna Universal One-Step NEB, Ipswich, USA) was used in PCR mixtures (20 µl ...
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bioRxiv - Genomics 2024Quote: ... The nodule-derived RNA was treated with NEBNext rRNA Depletion Kit (Bacteria) (NEW ENGLAND BioLabs) and RiboMinus Plant Kit for RNA-Seq (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... For this HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswitch, MA) was used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNAs were then further purified using Monarch® RNA Cleanup Kits (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: In vitro transcription was carried out using HiScribe® SP6 RNA Synthesis Kit (NEB, E2070S) in a 25 μl reaction containing 1 x SP6 Reaction Buffer ...
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bioRxiv - Genomics 2024Quote: ... T7 IVT was performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S) in a 60 μl total reaction per sample ...
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bioRxiv - Genomics 2024Quote: ... the reverse crosslinked nucleic acids were purified with Monarch RNA purification kit (NEB, 76307-460) and eluted into 21 µL of nuclease free water ...
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bioRxiv - Immunology 2024Quote: ... and subjected to library preparation using NEBNext Ultra II library prep kits (New England Biolabs). Libraries were quantified using the Jetseq library quantitation kit (Meridian Bioscience ...
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bioRxiv - Immunology 2024Quote: ... Purified round 2 PCR products were cloned using the NEB PCR Cloning Kit (NEB, #E1202) and sequenced with Sanger sequencing.
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bioRxiv - Genomics 2024Quote: ... and individually dual-barcoded using a New England Biolabs Ultra II Directional kit (NEB #E7765). Individually barcoded samples were pooled and sequenced paired end and 150 bp in length on two lanes of a Novaseq at the University of New Hampshire Hubbard Center of Genome Studies.
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bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) and cDNA was synthesized using the SuperScript IV First-Strand Synthesis System (18091050 ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs) following the manufacturer’s low DNA protocol ...
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bioRxiv - Cell Biology 2024Quote: RNA-sequencing libraries were prepared NEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB Cat #) followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA-seq libraries were generated using Next Ultra II Directional RNA Library Prep kit (NEB) and ribosomal RNA depletion according to the manufacturer’s protocols ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq library was constructed using NEBNext Ultra II RNA-library preparation kit (NEB, E7770S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and DNase was removed using the Monarch RNA Cleanup Kit (50 µg; New England Biolabs). LunaScript RT SuperMix Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the NEBuilder HiFi Master Mix was used according to kit instructions (E5520S, New England Biolabs). All single integration plasmids were digested with PmeI before transformations and integrations were verified by PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019) and were constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (New England Biolabs). For library amplification 17 or 24 PCR cycles were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthesis was performed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... Sequencing library was constructed using the NEB Ultra library preparation kit (New England Biolabs, Ipswitch).