Labshake search
Citations for New England Biolabs :
4701 - 4750 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The reverse-transcription (RT) reactions were performed with 2X LunaScript® RT SuperMix Kit (NEB) with 5 μL of RNA input in 10 μL total volume ...
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bioRxiv - Molecular Biology 2022Quote: The NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, Massachusetts, USA) was used to prepare 50 nt paired-end ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was first hybridized with the NEBNext rRNA Depletion kit v2 (NEB, cat# E7400) to diminish rRNA from the samples ...
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bioRxiv - Microbiology 2023Quote: ... rRNA was depleted by using the bacterial NEBNext rRNA Depletion Kit (New England BioLabs, E7850). Sequencing libraries were created by end repair and phosphorylation ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription (RT) was performed using ProtoScript First Strand cDNA Synthesis kit (New England Biolabs) to generate cDNAs ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using the Monarch® Total RNA Miniprep Kit (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... All PCR products were gel purified by using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 μL nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated using the Monarch Total RNA Miniprep Kit (New England BioLabs, cat #T2010) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... assembled them using a NEBridge Golden Gate Assembly Kit (BsmBI-v2) (#E1602S, New England Biolabs), and then electroporated 1 μL of the reaction into electrocompetent NEB5alpha ...
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bioRxiv - Molecular Biology 2023Quote: ... and the desired products were agarose gel purified using a Monarch gel extraction kit (NEB) and sequenced on an Illumina HiSeq.
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bioRxiv - Neuroscience 2023Quote: ... and 7f variants were derived by site-directed mutagenesis (NEB Q5 Site-Directed Mutagenesis Kit) of the GCaMP6s sequence in pDSP23 to reproduce the mutations described for GCaMP6f (pDSP31 ...
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bioRxiv - Genomics 2023Quote: ... All libraries were generated with NEBNext Ultra II Directional RNA library prep kit (NEB #E7760).
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bioRxiv - Biophysics 2023Quote: ... Derivatives of pSB963 were altered using mutagenic primers and Q5 site-directed mutagenesis kit (NEB) to generate vectors containing CEN9 ...
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bioRxiv - Cell Biology 2023Quote: ... the MBNL1 binding site (TGCTGC) was deleted using Q5 Site Directed Mutagenesis kit (NEB E0554). 12.5 ng of pFlareA-Mapt Ex4 splicing reporter was used as template and mixed with 12.5 µl of Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA libraries were prepared using NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) with polyA enrichment and sequenced on a Novoseq S4 sequencer (Illumina ...
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bioRxiv - Genomics 2023Quote: ... The sequencing library was constructed using an NEBNext DNA library prep kit (New England Biolabs), and 2×150 bp paired-end reads were sequenced using an Illumina NovaSeq 6000 by Novogene Corporation Inc ...
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bioRxiv - Biochemistry 2023Quote: ... or HiScribe® T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S) following the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: ... the indexed cDNA libraries were prepared using the NEBNext rRNA Depletion Kit (New England BioLabs), followed by NEBNext Ultra II Directional RNA Library Prep ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed site-directed mutagenesis using Q5 site-directed mutagenesis kit (New England Biolabs Inc.) in the pMSCV-Abi1 Isoform 2 DNA plasmid and point mutated tryptophan at amino acid 485 in the SH3 domain to glutamine ...
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bioRxiv - Microbiology 2023Quote: ... The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank) was gel extracted (Monarch DNA GEL Extraction Kit, NEB) and added to competence peptide stimulated S ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Plant Biology 2023Quote: ... followed directly by using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (NEB #E7600S ...
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bioRxiv - Synthetic Biology 2023Quote: ... IVT mRNA was produced using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040) along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs). All DNA was sequence verified using Sanger nucleotide sequencing (Retrogen ...
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bioRxiv - Plant Biology 2023Quote: ... The point mutant K665A SPY was generated via Q5 Site-Directed Mutagenesis Kit (NEB, E0552S). All plasmids were sequenced to ensure accuracy.
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bioRxiv - Plant Biology 2023Quote: Libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645). All ChIP-Seq libraries were sequenced on an Illumina NextSeq500 platform.
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bioRxiv - Physiology 2023Quote: ... Mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was concentrated using the Monarch PCR and DNA Cleanup kit (New England Biolabs, MA) with warmed elution buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from subconfluent cells with the Monarch total RNA purification kit (NEB), and reverse transcribed with LunaScript RT SuperMix (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared with NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science ...
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bioRxiv - Evolutionary Biology 2023Quote: ... size selection and PCR enrichment using the NEBNext Ultra Directional RNA Library Prep Kit (NEB). PCR enrichment step was performed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the amplified oligo pool was gel- purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the linearized vector for the light chain variant library ...
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bioRxiv - Neuroscience 2023Quote: ... using the NEBNext Ultra II RNA Library Prep with Purification Beads Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and libraries were prepared with a NEBNext Ultra II Library Prep Kit (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... hansenii NQ5 was isolated using the Monarch HMW DNA Extraction Kit for Tissue (NEB T3060S) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were edited via site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554).
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bioRxiv - Systems Biology 2023Quote: ... The resulting amplicon was purified by the Monarch PCR & DNA Cleanup Kit (New England Biolabs) and cloned into the pRDA-550 vector by NEBridge® Golden Gate Assembly Kit (BsmBI-v2 ...
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bioRxiv - Cell Biology 2023Quote: ... and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs). cDNA was sequenced using paired-end 150 bp chemistry on a HiSeq 4000 System (Illumina ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Biochemistry 2022Quote: ... All hAtg3 mutants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with corresponding primers listed in Extended Data Table 2 and verified by sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... and E779W were generated with the Q5 Site-Directed Mutagenesis Kit (E0554, New England Biolabs). The primers were designed with the NEBaseChanger® tool (Table S8).
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bioRxiv - Neuroscience 2023Quote: ... using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB E7760), and following the standard protocol ...
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bioRxiv - Biophysics 2023Quote: ... C5aR1 mutant was generated using NEB Q5 Site-Directed Mutagenesis Kit (NEB, Cat. no: E0554S).
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR Kit (E3005S, NEB). 20μl reactions using 200nM primers and <100ng RNA were run on ABI StepOnePlus Real Time PCR system following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were run using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a CFX384 (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... NGS sequencing libraries were generated using NEBnext Ultra II directional RNA library prep kit (NEB) following manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we have used NEBNext Ultra II DNA Library Prep Kits (E7645, New England Biolab NEB). We adopted the NEB protocol with some modifications ...
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bioRxiv - Genetics 2023Quote: ... and UltraTM II Directional RNA Library Prep Kit for Illumina® (NEB, cat no. E7760) with the following specifications ...