Labshake search
Citations for New England Biolabs :
4601 - 4650 of 9501 citations for FabFc ZAP rat Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Strand-specific RNA libraries were generated using the NEBNext Ultra II stranded library kit (New England Biolabs) combined with polyA-coupled beads (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was purified for all samples using a Monarch RNA Cleanup Kit (New England Biolabs, T2040L), according to manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA seq libraries were constructed from total RNA using the NEBNext Ultra II Directional RNAseq kit (NEB) with ribosomal RNA depletion ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting gRNAs were purified using the AMPure XP kit and treated with DNase I (NEB, UK). Concentration was evaluated on a Qubit system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420) at GenomeScan ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... RNA library preparation was done using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) on a Bravo WS automation system (Agilent) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Inc.) at Novogene and sequenced on an Illumina NovaSeq 6000 (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Immunology 2023Quote: ... IVT was done using the HiScribe® T7 High-Yield RNA Synthesis Kit (New England Biolabs, #E2040S), with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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bioRxiv - Genomics 2023Quote: ... Short-read libraries were prepared with the NEBNext Ultra II FS DNA Library Prep kit (NEB E7805), and sequenced 150 bp paired-end on an Illumina HiSeq3000 ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... using poly(A) selection and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). RNA-seq (2 × 100 nt paired-end reads ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were prepared for whole genome sequencing using an Epicentre Masterpure DNA Preparation Kit (Nordic Biolabs, Sweden). Genomic DNA libraries were constructed using Nextera XT Library Preparation Kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... The library was quantified using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs) by mixing 2 ul 1:10.000 diluted library with 8 ul Quant mastermix with added primers (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the Monarch genomic DNA extraction and purification kit (New England Biolabs Inc.). Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced with an Illumina HiSeq2500 (50 bp single-end) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA library was prepared using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) and NEBNext Multiplex Oligos for Illumina (NEB ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NEB Monarch Total RNA Miniprep Kit (New England Biolabs Cat #T2010S). RNAseq library prep was performed using NEBNext Ultra II RNA Library Prep for Illumina (NEB Cat# E7770S) ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Cat #E7645) following manufacture’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... NGS libraries were prepared using NEBnext Ultra II DNA library preparation kit for Illumina (New England Biolabs) and quantified with using a TapeStation (Agilent Genomics) ...
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bioRxiv - Cell Biology 2023Quote: ... Exon library was constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). DNA library hybridization was performed by using Agilent SureSelect Human All Exon V6 (Agilent) ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using a NEBNext Ultra II RNA Library Kit for Illumina (New England Biolabs) with the NEBNext Poly (A ...
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bioRxiv - Cell Biology 2023Quote: ... We created this deletion using a Q5 Site-Directed Mutagenesis kit (New England Biolabs, Cat. No: E0554S) with subsequent sequence verification ...
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bioRxiv - Genetics 2023Quote: ... 1x106 HEK293T cells were collected and their gDNA was extracted using the NEB Monarch kit (T3010S, NEB) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 4NBX.B WT sequence with C103A mutation was created by site-directed mutagenesis using commercially available kits (NEB). Smt3 SUMO protein with an N-terminal cysteine was created from wild type Smt3 SUMO by mutagenic PCR ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated gDNA was then prepared for epigenetic sequencing using the Enzymatic Methylation Sequencing Kit (EM-Seq) (NEB).49 Individual embryos were sequenced at a depth of 100 million reads using a 2 × 150 bp Illumina kit on the Illumina NovaSeq instrument ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed using the Luna® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB) with the CDC-derived primers for N1 and N2 gene targets and the reaction was performed using the QuantStudio™ 5 System (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... which was transcribed in vitro using the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs). Afterwards ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using an NEBNext rRNA Depeletion Kit (Bacteria) (New England Biolabs, Ipswich, MA). From the kit ...
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bioRxiv - Genetics 2023Quote: ... Molar concentration of libraries was determined using NEBNext® Library Quant Kit for Illumina® (NEB, #E7630) following manufacturer’s protocol for 6 Standards ...
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Genomic epidemiology and antifungal-resistant characterization of Candida auris, Colombia, 2016-2021bioRxiv - Genomics 2023Quote: ... Genomic libraries were constructed using NEBNext Ultra DNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) for Illumina and sequenced on Illumina Nova-Seq using NovaSeq 6000SP reagent kit (500 Cycles) ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs, Cat. no. E7805S) and unique dual indexes (Integrated DNA Technologies Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The plasmids carrying the desired inserts were transformed into strains by biolistic transformation as previously reported (82) ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The tps1Δ mutant was confirmed by PCR and the TPS1IDDΔ strain was confirmed by PCRs and sequencing to confirm the replacement of the CnTps1IDD with the CaTps1IDD ...
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bioRxiv - Cancer Biology 2024Quote: m6A seq was performed on 2×107 cells using EpiMark N6-methyladenosine enrichment kit (New England Biolabs). The libraries were prepared from purified DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... end-repair and A-Tailing were directly performed using the NEBNext UltraII DNA kit (New England Biolabs). A new custom universal Y-adapter based on the Illumina TruSeq sequences was designed to include a Unique Molecular Index (UMI ...
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bioRxiv - Cancer Biology 2024Quote: Total cellular RNA was isolated using the Monarch Total RNA Miniprep Kit (#T2010, NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from differentiated hiPSC-CMs using the Monarch Total RNA Miniprep Kit (NEB #T2010) and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000) ...