Labshake search
Citations for New England Biolabs :
4551 - 4600 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... complemented by reagents from the NEBNext Ultra II library prep kit (New England Biolabs, Ipswich, Massachusetts, U.S.A.), and then sequenced at a MinION platform (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA synthesis was performed with LunaScript RT SuperMix Kit (E3010L; New England Biolabs, Ipswich, MA), and 100 ng-1 μg total RNA was used for one 20 μl RT reaction ...
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bioRxiv - Biochemistry 2024Quote: ... All individual PLpro mutations were introduced into pDONR221-PLpro using NEB Q5 Site-Direct Mutagenesis Kit (NEB) following manufacturer’s instructions and shuttled into FU-tetO-Gateway-rtTA-2A-Puro using LR clonase.
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bioRxiv - Genetics 2023Quote: ... and genomic DNA libraries were prepared using the NEBNext genomic DNA library construction kit (New England Biolabs). DNA libraries were sequenced on an Illumina Hi-Seq and deep sequencing reads were analyzed using standard methods on Galaxy ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated gDNA was then prepared for epigenetic sequencing using the Enzymatic Methylation Sequencing Kit (EM-Seq) (NEB).49 Individual embryos were sequenced at a depth of 100 million reads using a 2 × 150 bp Illumina kit on the Illumina NovaSeq instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer instruction ...
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bioRxiv - Immunology 2023Quote: ... Immunoprecipiated DNA was used to prepare libraries using NEBNext Ultra II DNA library prep kit (NEB E7645S). Libraries were sequenced with paired-end 50 on an Illumina Novaseq.
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Molar concentration of libraries was determined using NEBNext® Library Quant Kit for Illumina® (NEB, #E7630) following manufacturer’s protocol for 6 Standards ...
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Genomic epidemiology and antifungal-resistant characterization of Candida auris, Colombia, 2016-2021bioRxiv - Genomics 2023Quote: ... Genomic libraries were constructed using NEBNext Ultra DNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) for Illumina and sequenced on Illumina Nova-Seq using NovaSeq 6000SP reagent kit (500 Cycles) ...
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bioRxiv - Physiology 2023Quote: ... and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA), with minor modifications to the manufacturers’ protocol (Kelley et al ...
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bioRxiv - Plant Biology 2024Quote: ... RNA sequencing was executed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: ... The oxidised PTC rRNA was subsequently purified from the reaction mixture with Monarch RNA Cleanup Kit (NEB) and immediately mixed with 150 nmol FTSC (300 eq. ...
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bioRxiv - Microbiology 2024Quote: ... Genomic libraries were prepared using the NEBNext Ultra II FS library preparation kit (New England Biolabs, USA) according to the manufacturer’s instructions for MiSeq (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... 20μL of DNA were used for libraries preperation using the NEBNext Ultra II DNA kit (NEB, #E7645). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-50 ng of purified RNA was processed by NEBNext rRNA Depletion Kit (New England BioLabs, Inc.) for removing ribosomal RNA ...
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bioRxiv - Microbiology 2024Quote: ... The SA pathway was then introduced into pGW44 using the Gibson Assembly Cloning Kit (New England BioLabs), creating plasmid pJM455 ...
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bioRxiv - Microbiology 2023Quote: ... and the DNA library was prepared using the NEBNext® Microbiome DNA Enrichment Kit (New England Biolabs). The sequencing run was then performed on the Element AVITI sequencer (Element Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... and TOP2B D64N self-trapping mutants were generated by the Q5 SDM Kit (NEB, catalog no. E0554S) following manufacturer’s instruction and mutation confirmation by sequencing ...
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bioRxiv - Genetics 2024Quote: ... CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; E7645L) and sequenced on NextSeq500 (paired-end ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II DNA library Kit (New England Biolabs GmbH, Frankfurt, Germany) with purification beads and 100 ng of the fragmented/non-fragmented DNA in a volume of 25 µl were used as input according to the manufacturer’s protocol with some alterations ...
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bioRxiv - Genomics 2024Quote: ... Ribosomal RNA was depleted from total input RNA using the NEBNext rRNA Depletion Kit (NEB cat. #E6310). First and second strand synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... and the vector was extracted from the gel using a Monarch DNA Gel Extraction Kit (NEB #T1020). Gibson Assembly of the three generated fragments was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) ...
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bioRxiv - Genomics 2024Quote: ... 200ng of each sample was enzymatically converted using the NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) with the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: Final libraries were prepared on beads using the NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
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bioRxiv - Immunology 2024Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesized using the ProtoScript First-Strand cDNA Synthesis kit (New England Biolabs). Real-time PCR assays were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... Strand-specific libraries were prepared by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB, USA), as described previously (Makhnovskii et al. ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs, Cat. no. E7805S) and unique dual indexes (Integrated DNA Technologies Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The tps1Δ mutant was confirmed by PCR and the TPS1IDDΔ strain was confirmed by PCRs and sequencing to confirm the replacement of the CnTps1IDD with the CaTps1IDD ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The plasmids carrying the desired inserts were transformed into strains by biolistic transformation as previously reported (82) ...
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bioRxiv - Cancer Biology 2024Quote: m6A seq was performed on 2×107 cells using EpiMark N6-methyladenosine enrichment kit (New England Biolabs). The libraries were prepared from purified DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... end-repair and A-Tailing were directly performed using the NEBNext UltraII DNA kit (New England Biolabs). A new custom universal Y-adapter based on the Illumina TruSeq sequences was designed to include a Unique Molecular Index (UMI ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs), profiled using the Agilent TapeStation D1000 high sensitivity ScreenTape on the Agilent 4150 TapeStation System ...
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bioRxiv - Cancer Biology 2024Quote: Total cellular RNA was isolated using the Monarch Total RNA Miniprep Kit (#T2010, NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from differentiated hiPSC-CMs using the Monarch Total RNA Miniprep Kit (NEB #T2010) and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000) ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR products were purified using the Monarch DNA and PCR Cleaning kit (New England Biolabs #T1030S) and templates were digested using Dnp1 (New England Biolabs #R0176) ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmids with various mutations were generated by PCR methods using Q5® Site-Directed Mutagenesis Kit (NEB) according to the instructions and verified by Sangon ...
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bioRxiv - Biochemistry 2024Quote: ... The transcription process was driven by T7 polymerase using HiScribe T7 High Yield RNA Synthesis Kit (NEB). After 2h transcription ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). Target sequences were amplified using a modified touchdown PCR protocol (Korbie and Mattick ...
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bioRxiv - Synthetic Biology 2024Quote: ... Sequencing libraries were generated using NEB Next® UltraTM DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and index codes were added ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA isolated from cells was reverse transcribed using LunaScript® RT SuperMix Kit (New England Biolabs) to generate cDNA as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs) and quantified using the Qubit Broad Range dsDNA kit (ThermoFisher) ...