Labshake search
Citations for New England Biolabs :
4401 - 4450 of 9425 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and used to prepare the library with the Next ultra II DNA library prep kit (E7645, NEB) using 1 μg of DNA per sample as input ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Biochemistry 2024Quote: ... The inserts were each ligated with digested pmirGLO plasmids using a quick ligasation kit (New England Biolabs) and transformed into JM109 competent cells (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were then isolated using the Monarch® Plasmid Miniprep Kit (New England Biolabs, Ipswich, MA, USA) and used to transfect HEK293T cells (ECACC ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from ∼2×106 cells using the Monarch DNA purification Kit (New England Biolabs). For genotyping ...
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bioRxiv - Genetics 2023Quote: ... and genomic DNA libraries were prepared using the NEBNext genomic DNA library construction kit (New England Biolabs). DNA libraries were sequenced on an Illumina Hi-Seq and deep sequencing reads were analyzed using standard methods on Galaxy ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... All individual PLpro mutations were introduced into pDONR221-PLpro using NEB Q5 Site-Direct Mutagenesis Kit (NEB) following manufacturer’s instructions and shuttled into FU-tetO-Gateway-rtTA-2A-Puro using LR clonase.
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was done using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs) as described by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... The transcription of guide RNAs (gRNAs) was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Genetics 2023Quote: ... Whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs, E7645L) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: Library construction was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB). 4ng of fragmented DNA was used for end-repair/A-tailing ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was generated using an AMV first strand cDNA synthesis kit (New England Biolabs, Catalog # E6550). The segment was amplified through PCR with PstI and BamHI restriction sites incorporated onto the double-stranded cDNA using specific primers (PstI-VIGS-F and BamHI-VIGS-R ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligation of the insert to the vector was performed using the Quick Ligation kit (New England Biolabs). The final vector was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into miniTn7/pMQ56 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... NCPF 8977 (aggregating) and NCPF 13029 (weakly-aggregating) using Monarch RNA extraction kit (NEB cat no. T2010) as per manufacturer’s specification ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription for first-strand cDNA synthesis was performed by using the LunaScript RT SuperMix Kit (NEB) containing both oligo(dT ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (500 μg) (Catalog No. T2050L, New England BioLabs) and concentration was measured using a NanoDrop ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enyzme were purchased from New England BioLabs (NEB, Germany). PCR was performed using Q5 High Fidelity 2X Master Mix (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2023Quote: MeRIP sequencing (MeRIP-seq) libraries were prepared using the Ultra II directional RNA library preparation kit (NEB) as per manufacturer’s instructions for already fragmented samples ...
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bioRxiv - Immunology 2022Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), then treated with ExoSAP-IT (Affymetrix ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared using NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504 ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were depleted three times of polyA(+) RNA using the Magnetic mRNA isolation kit (New England Biolabs), each time keeping the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and a 1:1 mix of oligodT and random hexamer primers to obtain cDNA ...
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bioRxiv - Microbiology 2023Quote: Total RNA from cell pellets was extracted using a Monarch Total RNA Miniprep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
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bioRxiv - Genomics 2023Quote: ... Transcriptome libraries were constructed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, UK) according to the manufacturer’s instructions and sequenced with 150-bp paired-end sequencing using the Illumina NovaSeq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... and adaptor ligation was performed on beads with the NEBnext Ultra II kit (New England Biolabs E7645). An optimal PCR cycle for final library amplification using NEBnext Ultra II Q5 was determined ...
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bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was enriched from 400ng of total RNA using the NEBNext rRNA Depletion Kit v2 (NEB, # E7400X) to remove ribosomal RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-free libraries were prepared with the NEBNext UltraTM II DNA Library Prep Kits (New England BioLabs) according to supplier recommendations ...
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bioRxiv - Genomics 2023Quote: ... The UMI-4C sequencing library preparation was obtained using the NEBNext Ultra II Library Prep Kit (NEB). Library amplification was performed by nested PCR ...
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bioRxiv - Immunology 2023Quote: ... expression levels were determined using the Luna Universal One-Step RTqPCR kit (New England Biolabs catalog #E3005X) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Mutations were introduced by in vitro mutagenesis using Q5 Site-directed Mutagenesis Kit (New England Biolabs, #E0554S) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... rLCMV-GP1-FLAG was generated by insertion PCR utilising Q5 site-directed mutagenesis kit (New England BioLabs) and FLAG primers ...
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bioRxiv - Microbiology 2023Quote: ... Changes were introduced into the env genes using a Q5 site-directed mutagenesis kit (New England BioLabs). The presence of the desired mutations was confirmed by DNA sequencing.
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bioRxiv - Microbiology 2023Quote: ... followed by library preparation with NEBNext Ultra DNA v2 Library Prep Kit for Illumina (New England Biolabs). Library preparation included 7 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng DNA was used to prepare libraries using the NEBNext Ultra II DNA kit (NEB E7645S) as described with modifications ...
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bioRxiv - Synthetic Biology 2023Quote: RNA samples were converted to cDNA using the Protoscript II First Strand cDNA Synthetsis Kit (NEB, E6560S) following the supplier’s standard protocol instructions ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using NEBNext® μLtra™ DNA Library Prep Kit for Illumina (NEB, USA) using 1.0 μg DNA per sample ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were then used for library preparation using NEBNext UltraII DNA library prep kit (NEB #E7645L). Paired end sequencing of the libraries was performed using Illumina NextSeq 550 platform and atleast 3 million paired end reads were obtained for each sample ...