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Citations for New England Biolabs :
401 - 437 of 437 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The cell lysate was collected and incubated for 1 h with 2 ml of 50% slurry of chitin resin (New England Biolabs) before loading onto an empty EconoPac gravity-flow column (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was clarified by centrifugation at 5°C at 18 500 rpm for 30min and loaded onto gravity flow amylose resin (NEB) previously equilibrated with buffer WB1_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2023Quote: Purified CBC or CBC-ARS2147-871 and their mixtures with different His-MBP-tagged partners were loaded onto Amylose resin columns (NEB). Columns were then extensively washed with a buffer containing 100 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... The mixtures were diluted into 400 µl of Binding Buffer and then incubated with the appropriate resin (amylose for MBP, New England Biolabs # E8021S ...
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bioRxiv - Plant Biology 2023Quote: ... The MBP-OsTLP protein or the MBP control protein was bound to amylose resin (New England Biolabs, Beverley, MA, USA), and then the LssaCA-His protein was added to the beads ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was applied to Glutathione Sepharose 4B affinity resin (1 ml bed volume per 2 l culture; New England Biolabs), and incubated with rotation for
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bioRxiv - Cell Biology 2023Quote: ... The vector was transformed into BL21-DE3 cells and the protein purified using gravity flow amylose resin (New England Biolabs) affinity chromatography ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested and lysed as described above and were run over 5 ml of Amylose affinity resin (New England Biolabs), washed with 100 ml of 20 mM sodium phosphate pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 mM MgCl2) for 20 mins at room temperature and then subjected to MBP pulldown using Amylose Resin (New England Biolabs). The bound DNA was recovered using proteinase K (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... Cleared lysate containing the nucleosomes was incubated with purified MBP-tag or MBP-FoxP3ΔN protein (1uM) for 1 hour at 4℃ and then subjected to MBP pulldown using Amylose Resin (New England Biolabs). After proteinase K (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... the FIP200-MBP-ATG13-ATG101 eluate and ULK1 eluate were mixed to allow reconstitution of the complex and loaded onto amylose resin (New England Biolabs). After 4 hours of incubation with the beads and extensive washing in washing buffer (20 mM HEPES pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were incubated for 1 hour at 4 °C on a nutator with 40 µL amylose resin slurry (New England Biolabs) equilibrated with amylose wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... His-MBP-Mis18BP120-130 was purified using the same lysis buffer containing 500 mM NaCl and purified using amylose resin (NEB). Proteins were then eluted by an elution buffer containing 10 mM Maltose.
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bioRxiv - Biochemistry 2024Quote: ... MBP-PR18, MBP-TEV-FUS, and MBP-hnRNPA2 (NLS, residues 298‒336) were purified using affinity chromatography (Amylose resin, NEB), and MBP-PR18 was further purified using cation exchange chromatography (HiTrap SP HP ...
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bioRxiv - Biophysics 2024Quote: ... In vitro pull-down binding assays were performed in triplicate by incubating proteins at their indicated concentrations with 20 µL amylose resin (bead bed volume; New England Biolabs) in a 200 µL reaction in Assay Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was clarified by centrifugation at 30,000 g for 30 min and the supernatant was incubated with amylose resin (NEB #E8021) for 60 min with rotation ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant fluids containing GST-fused proteins or MBP-fused proteins were incubated with Glutathione Sepharose 4B beads (#17-0756-01; Cytiva) or amylose resin (#E8021; New England Biolabs), respectively ...
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bioRxiv - Systems Biology 2021Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volume cold wash buffer before fractionated elution in a buffer containing 25 mM Hepes pH 7.5 ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and either BICD2N-Halo (50 pmol) or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281; Chitin, New England BioLabs, S6651 ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was collected after centrifugation at 18,000 rpm for 30 minutes and then incubated with amylose resin (New England BioLabs, Ipswich, MA) for 2 hours ...
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bioRxiv - Microbiology 2021Quote: The fusion protein was purified from the clarified supernatants by adding 20 ml of chitin resin (New England Biolabs, cat#S6651S) and incubating with gentle rotation overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volume cold wash buffer before fractionated elution in a buffer containing 25 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... the TEV protease was removed by incubation of the purified protein with 250 µL amylose resin (New England Biolabs, Hitchin, UK). Protein concentrations were determined by the bicinchoninic acid assay (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... TSF-ATG101:ATG13 (1-197) complex (final 3 μM) were incubated with 30 μl Amylose resin (New England Biolabs, Ipswich, MA) at 4 °C overnight in the ITC buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Strep–PIF4 recombined proteins were expressed in the Escherichia coli BL21 (DE3) strain and then purified using amylose resin (NEB, E8021S). DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 16,000 × g for 45 min and the supernatant was loaded onto amylose affinity resin (New England BioLabs, Ipswich, MA). The column was washed with 10 CVs of lysis buffer and the protein was eluted with sequentially added 1 CV aliquots of lysis buffer Supplemented with 20 mM maltose ...
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bioRxiv - Systems Biology 2022Quote: ... The Ni-elution was collected directly on a pre-equilibrated amylose column (amylose high flow resin, New England Biolabs, Ipswich, Massachusetts). Amylose column was washed with 5 column volumes of cold wash buffer then eluted in a buffer containing 25 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation at 214,743 rcf at 4°C in S55A rotor using a Sorvall Discovery M120 SE Micro-Ultracentrifuge and added to 1 mL of Chitin Resin (NEB # S6651S) in a Peirce centrifugation column (Thermo Fisher # 89897 ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs, Ipswich, US) and the concentration was determined using the Bradford technique ...
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bioRxiv - Biophysics 2022Quote: ... A low-pressure chromatography column connected to a peristaltic pump (5 mL/min flow rate) was prepared with 5 mL amylose resin (NEB #E8021S), followed by equilibration with 50 mL Amylose A Buffer ...
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bioRxiv - Microbiology 2024Quote: ... The soluble fraction was collected by high-speed centrifugation at 12,000 x g for 1 hr and applied to an Amylose resin high-flow column (NEB Cat#E8022S). Unbound proteins were removed by washing with sonication buffer ...
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bioRxiv - Biochemistry 2024Quote: ... whereas Benzonase-MBP was purified from outer membrane lysates obtained as with CXCL12 and isolated with use of amylose resin (NEB, E0821) as per manufacturer protocols ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... or CBD-HAP1CC1 (100 pmol) was incubated with resin (Magne® HaloTag, Promega, G7281; Chitin, New England BioLabs, S6651; or Amylose, New England BioLabs, E8021) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and the impurities that were tagged by Chitin Binding Domain were extracted on a gravity-flow column using 5 mL Chitin resin (New England BioLabs, Evry, France). The target proteins were purified with HisTrap HP columns (GE Life Sciences ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... GGGMcm3 (in association with the other Mcm2-7 subunits) was cleaved off the resin with 500 units of 7×His-TEV protease (NEB; rotation overnight at 4°C). The flow-through was collected and applied to 0.4 mL volume Ni-NTA Agarose resin (Qiagen ...