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Citations for New England Biolabs :
301 - 350 of 437 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The pre-mRNA-containing fractions were concentrated by binding to amylose resin (New England Biolabs). For SDS PAGE analysis ...
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bioRxiv - Plant Biology 2020Quote: ... MBP and GST fusion proteins were separated from supernatants using amylose resin (New England Biolabs) and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences) ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: Lysates were incubated with 15 μL of lysis buffer-equilibrated amylose resin (New England Biolabs) for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns of MBP-tagged fusion proteins were performed using an amylose resin (New England Biolabs). Appropriate amounts of (see Fig ...
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bioRxiv - Developmental Biology 2021Quote: ... The lysate was clarified by centrifugation and incubated with amylose agarose resin (New England Biolabs) for 1 hour before washing the resin with ≥100 column volumes of lysis buffer and then eluted with ten column volumes of lysis buffer supplemented with 20 mM maltose ...
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bioRxiv - Microbiology 2022Quote: ... Poly-Prep chromatography columns were loaded with 300 µl of amylose resin (New England Biolabs). After columns were washed 3X with 10 ml of cold Buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... The crude extracts were loaded into a column containing amylose resin (E8021S, New England Biolabs) for affinity chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Neuroscience 2023Quote: ... 20 mM TrisCl (pH 7.6)) and applied to Amylose resin (New England BioLabs, lpswich, MA). The purified MBP-lgMPZ fusion protein was eluted with column buffer containing 10 mM maltose and stored at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 200 pmol MBP or MBP-Mps11–250 was immobilized on amylose resin (New England BioLabs) equilibrated in lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... and affinity purified from Escherichia coli strain BL21 (DE3) using Amylose Resin (New England Biolabs) as previously described 5.
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bioRxiv - Cell Biology 2023Quote: ... purified using amylose resin according to the protocol provided by the manufacturer (New England Biolabs), and sent to Pocono Rabbit Farm and Laboratory for polyclonal antibody generation in rabbits ...
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bioRxiv - Molecular Biology 2023Quote: ... a self-packed XK column (Cytiva) with 15 mL of Amylose resin (New England Biolabs) was attached to the HisTrap column ...
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bioRxiv - Immunology 2023Quote: ... The diluted supernatant was loaded onto a 5 mL amylose resin column (New England Biolabs). The column was washed with 150 mL of buffer I ...
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... MBP-ICD (and associated W22R “black” mutant) was purified through amylose resin (New England Biolabs), followed by cation exchange chromatography using a Source 15S column (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was collected and incubated with NEBExpress Ni Resin (New England Biolabs, Cat# S1428S) overnight at 4° C to remove the TEV protease from the purified protein ...
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bioRxiv - Molecular Biology 2024Quote: ... A self-packed XK column (Cytiva) with 15 mL of Amylose resin (New England Biolabs) was attached to the HisTrap column ...
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bioRxiv - Plant Biology 2024Quote: ... Sonicated samples were used for purification by affinity chromatography using amylose resin (New England Biolabs) for MBP-NAC53/78 or glutathione sepharose 4B (cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was collected and incubated with 10 ml of Amylose resin (New England Biolabs) for 90 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... A self-packed XK column (Cytiva) with 15 mL of amylose resin (New England Biolabs) was attached to the HisTrap column which was then equilibrated into Lysis buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The remaining supernatant was incubated with 1 ml of amylose resin (NEB, Ipswich, Massachusetts, USA) rotating for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... This was added to 5 ml (per 2 L of culture) amylose resin (New England Biolabs) equilibrated in lysis buffer and left on a tube roller shaker at 4 °C for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... the supernatant was collected and mixed with 1 ml of amylose agarose resin (New England Biolabs) pre-equilibrated with buffer A per 50 ml of cell homogenate ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... The fusion protein was purified from the supernatant using amylose resin (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 RBD were conjugated to SNAP-Capture Pull-Down resin (New England BioLabs). For each conjugation ...
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bioRxiv - Genomics 2022Quote: ... Tn5 transposase was bound by mixing the cleared lysate with 30 mL of chitin resin (NEB) in a column and rotating end-to-end for 30 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were eluted with lysis buffer + 250 mM imidazole and bound to chitin-resin (S6651L, NEB), before washing in lysis buffer and overnight incubation with lysis buffer + 200 mM DTT and his-ULP1 protease ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant protein of AP2 domains fused with MBP (MBP::PbSIP2) was purified using amylose resin (NEB) and recovered in 10 mM maltose solution.
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bioRxiv - Molecular Biology 2024Quote: ... MBP-fusion proteins were affinity purified with amylose resin following the manufacturer’s instructions (New England Biolabs). The identity and purity of recombinant purified MBP-fusion proteins were confirmed by SDS-PAGE and western immunoblotting analyses.
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was then incubated with amylose affinity resin (NEB, 2mL bead volume/1L bacterial culture) for 1hour at 4°C while nutating ...
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bioRxiv - Synthetic Biology 2024Quote: ... pH 7.5) and subjected to purification using 1 mL of Amylose Resin (New England Biolabs, Japan). The column was equilibrated with 5 column volumes (CV ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterial cells were lysed by sonication and MBP fusions were purified onto amylose resin (New England Biolabs) as previously described (Ten Hoopen et al. ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using a pre-poured amylose column containing 4 mL amylose resin (New England Biolabs, E8021L) followed by size exclusion chromatography (protein buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was resuspended in 100 μL of NLS Buffer and 10 μL of Proteinase K (NEB) and the sample was incubated at 50°C for 30 minutes while shaking at 1200 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... The NRPD1-Maltose Binding Protein (MBP) fusion protein was affinity purified using Amylose resin (New England Biolabs) and eluted with 20 mM maltose ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was incubated with gentle shaking for 1 h with 10 mL of amylose resin (NEB) pre-equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: Following IMAC samples were processed and applied to chitin resin according to manufacturer’s instructions (New England Biolabs). The CCC flow-through harbouring PA4130 was collected and analysed by SDS-PAGE for homogeneity ...
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bioRxiv - Plant Biology 2022Quote: ... The soluble fraction of MBP-MP was applied to pre-equilibrated amylose agarose resin (New England Biolabs). After washing the column three times with amylose column buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was dripped twice through a 10 ml packed volume of amylose resin (New England Biolabs), washed with 5 column volumes of column buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was incubated with 400 μl bed volume of pre-washed amylose resin (New England Biolabs) for 18 h ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...
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bioRxiv - Plant Biology 2023Quote: ... and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Biophysics 2023Quote: ... The clarified and filtered lysate was loaded on 1 mL of IgG Sepharose fast flow resin (NEB). The column was washed with lysis buffer and TEV cleavage buffer (lysis buffer with 75 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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bioRxiv - Cancer Biology 2023Quote: ... Cleared bacterial lysate from 1L of culture was incubated with 2mL of equilibrated amylose resin (NEB #E8021L) in a rotator overnight at 4C ...
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bioRxiv - Biochemistry 2023Quote: ... the resin was mixed with 10 μM SNAP-Cell® TMR-Star (New England Biolabs Inc., #S9105S) at room temperature for 10 minutes to label the SNAPf-tag ...