Labshake search
Citations for New England Biolabs :
351 - 400 of 437 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... MBP-FIP200 (1–634) was purified by affinity chromatography using Amylose Resin High Flow (New England Biolabs). Next ...
-
bioRxiv - Cell Biology 2020Quote: ... The extract was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, Ipswich, MA), loaded onto a column ...
-
bioRxiv - Plant Biology 2021Quote: ... 30 µl was removed (“input”) and the remaining sample was mixed with 50 µl of Amylose Resin (NEB) in a final volume of 500 µl ...
-
bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using amylose resin (New England Biolabs). Following washing with buffer K-75 ...
-
bioRxiv - Cell Biology 2022Quote: ... and isolated and affinity-purified using amylose resin according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA). Polyclonal antisera were generated by immunization of 6-weeks old female NMRI mice (Charles River Laboratories ...
-
bioRxiv - Physiology 2022Quote: ... Diluted lysate was then incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
-
bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...
-
bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
-
bioRxiv - Biochemistry 2021Quote: ... The second fraction of the clarified lysate was loaded onto 200 μl of Amylose Resin (New England Biolabs) equilibrated in Amy-Strep wash buffer (20 mM HEPES pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... the supernatant solution was incubated for at least 2□h with amylose-affinity chromatography resin (New England Biolabs), whilst gently shaking at 4□°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... MBP tagged protein was subsequently bound in batch for 1 h to 1 ml of Amylose resin (NEB) per litre of input culture (typically 4 ml total resin) ...
-
bioRxiv - Plant Biology 2024Quote: ... Supernatant was recovered after centrifugation (12000 xg, 4°C, 1h) and incubated with 0.002% equilibrated amylose resin (NEB) for 2h on a rotating wheel at 4°C ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were purified using the amylose resin affinity purification method by following the manufacturer’s recommendations (E8021L, NEB). Briefly ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was passed through a 0.22 μm syringe filter prior to adding to 0.5 ml NEBExpress Ni Resin (New England Biolabs) by gravity flow ...
-
bioRxiv - Cell Biology 2024Quote: ... the complex is collected by affinity purification using FIP200-MBP and incubating the complex with Amylose resin (BioLabs) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
-
bioRxiv - Bioengineering 2021Quote: ... and cleavage of the fusion protein with enterokinase that were performed according to manufacturer’s instructions (amylose resin purification – according to pMAL Protein Fusion & Purification System, New England Biolabs, Ltd. ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was passed three times over a column of 10 ml of amylose resin (New England Biolabs, E8031L). The column was washed with MBP wash buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates were cleared via centrifugation at 30,597xg and 4°C for 20 min and the supernatant was applied to amylose resin (NEB). The column was washed with 15 column volumes (CV ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4 C ...
-
bioRxiv - Molecular Biology 2021Quote: ... The soluble extract obtained by centrifugation was supplemented with 0.01% NP40 and incubated with amylose resin (New England Biolabs) at 4°C for 1 h ...
-
bioRxiv - Biophysics 2020Quote: ... MBP-GLIC was eluted using 2 column volumes of Buffer B1 with 300 mM imidazole and 0.02% DDM and then applied to an amylose resin column (New England Biolabs). Amylose resin column bound to MBP-GLIC was washed with 2 column volumes of Buffer B1 with 0.02% DDM and MBP-GLIC was eluted using 2 column volumes of Buffer B1 with 20mM maltose and 0.02% DDM ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was isolated by centrifugation at 90,000 g for 35 min and the solubilized complex was incubated with amylose resin (NEB) for 2.5 h at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... Insoluble material was pelleted at 119,000g for 35 minutes and the supernatant was bound to amylose resin (New England Biolabs). The resin was washed with lysis buffer and eluted with lysis buffer plus 10 mM maltose ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates were centrifuged for 15 min at 30,700 x g and the collected supernatants were incubated with amylose resin (New England Biolabs) for 1 h at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Par-3 PDZ1-APM and PDZ1-APMΔPDZ2 were first his-purified (described above) prior to incubation with amylose resin (NEB). Amylose-bound Par-3 was then washed and resuspended in binding buffer as described for GST proteins.
-
bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen ...
-
bioRxiv - Biophysics 2020Quote: ... After 1 hour of centrifugation at 100,000xg at 4 °C the supernatant was incubated with Chitin resin (New England Biolabs) for 30 minutes at 4 °C to remove metalloproteases ...
-
bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
-
bioRxiv - Biochemistry 2023Quote: ... The clarified supernatant was then batch-bound for 3 hours on to 20 mL chitin resin (New England Biolabs) equilibrated with 200 mL of cell lysis buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
-
bioRxiv - Plant Biology 2024Quote: ... The filtered lysate was passed over a gravity-flow column packed with 0.5 ml amylose resin (New England Biolabs) that had been pre-equilibrated with extraction buffer ...
-
bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
-
Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complexbioRxiv - Biochemistry 2023Quote: Pull-down binding assays were performed by immobilizing purified His6MBP or His6MBP-Kap114 on amylose resin (New England BioLabs). Resin was stored in Binding Assay (BA ...
-
bioRxiv - Biochemistry 2023Quote: ... In order to remove the EndoHf from the HPAMPD the supernatant was brought to 100 mM Tris -HCl pH 7.5 and 100 mM NaCl and applied to a one ml amylose resin (NEB) column at a rate of about 1 drop per second at 25° C ...
-
bioRxiv - Biochemistry 2024Quote: ... and clarified by centrifugation at 16,100 x g for 1hr and incubated with ∼1 mL of pre-equilibrated compact amylose affinity resin beads (NEB). The resin was washed three times with buffer H ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation at 100,000 × g for 30 min and incubated with amylose affinity resin (New England BioLabs). Resin was washed with lysis buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... Lysate was clarified by centrifugation at 100,000 × g for 30 min and incubated with amylose affinity resin (New England BioLabs). Resin was washed with lysis buffer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Ni-NTA resin elute was immediately diluted with PBS containing 1 mM EDTA (PBS-E) and incubated with amylose resin (New England Biolabs) for 30 min ...
-
bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 15,000 x g for 30 min at 4°C and the clarified extract was loaded onto a chitin resin (NEB) column pre-equilibrated with column buffer for purification at RT ...
-
bioRxiv - Biophysics 2021Quote: ... The unbound MBP or 4 MBP that remained in the unbound fraction was then loaded on Amylose resin (New England Biolabs) previously equilibrated in buffer D ...
-
bioRxiv - Biochemistry 2021Quote: ... Supernatants were adjusted to 300 mM NaCl and 2 mM DTT and were incubated with 500 μL of amylose resin (New England Biolabs) pre-equilibrated with binding buffer (50 mM HEPES-NaOH pH 7.2 ...
-
bioRxiv - Microbiology 2020Quote: ... at 98,000 × g at 4 °C for 45 min and the supernatant loaded onto a self-packed column with ∼10 ml of amylose resin (NEB) pre-equilibrated in 50 mM Tris-HCl pH 9.0 ...
-
bioRxiv - Biophysics 2020Quote: ... The lysate was cleared from cell debris by centrifugation at 50000 g for 1 hour followed by incubation with Amylose resin (NEB) for 1 hour ...
-
bioRxiv - Physiology 2022Quote: ... 1 L of diluted extracts were incubated with 9 ml of packed amylose resin (catalog no. E8021S, New England Biolabs) and incubated at 4°C for 2h with gentle rotation ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μl of MBP-SYCP1N at 2 mg/ml was incubated for 20 minutes on ice with 100 ul amylose resin slurry (NEB) equilibrated in 20 mM Tris pH 8.0 ...
-
bioRxiv - Microbiology 2020Quote: ... fusion protein was purified by affinity solid phase extraction on an amylose resin as suggested by the manufacturer (New England Biolabs). Rabbits were immunised by intramuscular injections of 400 μg purified MBP–A104R fusion protein every four weeks over a period of four months ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein expression was induced with 0.3 mM IPTG for 2 hours at 37°C and recombinant MBP-rabaptin5 was purified with an amylose resin (New England Biolabs) according to manufacturs instructions and dialysed against lysis buffer (20 mM Tris-HCl ph7.4 ...
-
bioRxiv - Biochemistry 2020Quote: ... eluted protein samples may be treated with TEV protease at 4 °C overnight before flowing through Amylose resin (New England Biolabs) for a second step of affinity purification ...