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Citations for New England Biolabs :
4401 - 4450 of 5199 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The treated RNA samples were incubated with 100 μM RNA rP5_RND oligo (final 10 μM, Table S3) 2h at 25°C with 10 Units of T4 RNA ligase 1 (NEB). Please note that we used an RNA oligo ...
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bioRxiv - Biochemistry 2020Quote: ... SMN-Gemin2 complex was produced by co-expression of SMN (residues 1-294) and Gemin2 (12-280) fused to a C-terminal Mxe intein (NEB) containing a hexahistidine tag ...
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bioRxiv - Developmental Biology 2020Quote: ... 300ng of undigested gDNA and from each restriction reaction were loaded in a 1% agarose gel with molecular weight marker (N3232, NEB). 160V current was applied for 30min in a Midigel tank (370000 ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA (1 μg) or TRAP-isolated RNA (200 ng) was subjected to the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) to isolate mRNA and proceeded directly to double stranded cDNA synthesis ...
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bioRxiv - Plant Biology 2021Quote: ... Selected SNPs were converted to restriction enzyme-based Cleaved Amplified Polymorphic Sequence (CAPS) markers (Supplementary Table 1) using Neb cutter v2.0 (New England Biolabs Inc). Restriction digestion was performed according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial lysis was completed by incubating for 3 h at 55°C in the presence of 1% SDS and 100 μg/ml proteinase K (NEB). Lysates from both methods were extracted twice with equal volumes of phenol– chloroform–isoamyl alcohol (25:24:1) ...
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bioRxiv - Biophysics 2021Quote: ... and 15–25 μM BG-oligonuculeotides were labeled with ∼1 μM myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer for 30 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... cell pellets were washed 1× with PBS and collected in 1X DNA/RNA protection reagent (Monarch Total RNA Miniprep Kit, NEB). Cells were lysed and total RNA was isolated following the mammalian cell protocol inlcuding on-column DNase I treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA from the ChiP procedure (25 μL) was digested with 1 μL of I-SceI endonuclease (#R0694S, New England Biolabs) for 1 h and heat inactivated ...
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bioRxiv - Microbiology 2020Quote: ... but our procedure differed slightly as (1) we ‘padded’ a given sample with linear double-stranded lambda DNA (New England Biolabs) if the sample did not meet the manufacturer DNA input mass recommendations (1000 ng ...
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bioRxiv - Genomics 2020Quote: ... Purified fragments were mixed at 1:3 molar ratio and assembled with NEBuilder HiFi DNA Assembly Mater Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of eluted RNA was mixed with 1 μL of 200 ng/μL random nonamer primer (New England Biolabs), incubated at 65°C for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... the annealed primers and purified digested vector were ligated at a 15:1 insert to backbone molar ratio using Instant Sticky-end Ligase Master Mix (M0370S, NEB). A non-targeting control (miR-NTC ...
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bioRxiv - Genomics 2020Quote: ... and half of the DNA was 3’-end labeled for 1 h at 37 °C in a 10-μl reaction containing 6 units of terminal deoxynucleotidyl transferase (New England Biolabs), 0.25 mM CoCl2 ...
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bioRxiv - Genomics 2021Quote: ... The FPR cassette comprising NatMX-mCherry-PPT1/SUT129 promoter-YFP was excised from the backbone using AscI restriction enzyme (1 U per µg plasmid, NEB). Fragment I was the bidirectional GCG1/SUT098 or ORC2/SUT014 promoter sequences amplified by Phusion U polymerase from BY4741 genomic DNA ...
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs] ...
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bioRxiv - Genomics 2021Quote: ... “Fuorf_RNA_adapter” was ligated to the 3’ end of 10 μl of cDNA in a 20 μl reaction at 65 °C for 1 hour using Thermostable 5’ App DNA/RNA ligase (New England Biolabs). The reaction was inactivated at 95 °C for 3 minutes and 2 μl of the reaction was used as a template for PCR for sequencing library generation as described below.
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bioRxiv - Genetics 2020Quote: ... was annealed with 1ul of the reverse sgRNA (100 uM) using 1 ul 10X T4 ligation buffer (New England Biolabs, NEB), 1 ul T4 PNK (NEB) ...
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bioRxiv - Genetics 2021Quote: Plasmid pNR127.9 was constructed by changing the first rfk-1 stop codon in pNR9.1 from TAG to TGG with primer set 1727/1728 and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Similarly ...
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bioRxiv - Neuroscience 2020Quote: ... FP: 5’-AGCAAGGCTAGCCAAGACAAGTTTGTAC-3’ and RP: 5’-ACTCACGGGCCCTAGTGGGCAGATCTT-3’ and cloned between Nhe-1 and Apa1 (NEB, Ipswich, MA, USA) sites in mec4p::Lamp-1::GFP.
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bioRxiv - Cell Biology 2020Quote: ... A fragment of human Plexin-B1 cDNA (encoding amino acids 1-535) was cloned into the pcDNA5 vector using HindIII (R0104S, NEB) and XhoI (R0146S ...
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bioRxiv - Cell Biology 2021Quote: ... The β1 Integrin-HaloTag-GFP without ARL13B–C terminus (I-GFP) was generated by Q5 site directed mutagenesis (New England Biolabs).
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bioRxiv - Physiology 2021Quote: ... RNA (0.5-1 μg) was reverse-trancribed to cDNA using LunaScript RT SuperMix Kit (New England Biolabs, Ipswich, MA, USA). TaqMan gene expression assays with FAM-labeled probes were purchased from ThermoFisher Scientific (Carlsbad ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribosome concentrations were calculated with a NanoDrop spectrophotometer assuming 1A260 = 60 μg ribosome mL−1 (conversion factor provided by New England Biolabs). This conversion factor was used to estimate the molecular mass of bacterial ribosomes ...
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bioRxiv - Physiology 2021Quote: ... ChIP-seq libraries were prepared from 1 to 5 ng eluted DNA using NEBNext Ultra II DNA library Prep Kit (New England BioLabs), with 12 cycles of library amplification ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB). PCR primers are listed in Supplemental Table 2 ...
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bioRxiv - Biochemistry 2021Quote: The α-(1,2)-Galf-containing N-linked glycans were released from 1 mg Transglucosidase L “Amano” (Amano) by using PNGase F (New England Biolabs) under denaturing conditions ...
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bioRxiv - Biochemistry 2021Quote: ... per 1g of wet cell weight and 1 Unit of 30 Units/μL RNase Inhibitor (from human placenta; New England Biolabs (NEB), No ...
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bioRxiv - Microbiology 2021Quote: ... 15 Plasmids encoding agrD-I(1-32)-gyrA-His7 and agrD-III(1-32)-gyrA-His7 thiolactone were cloned between the NdeI and SapI sites of a modified pTXBI (IMPACT) vector (NEB), containing a C-terminal Mxe GyrA-His7 ...
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bioRxiv - Bioengineering 2020Quote: ... and mRNA was isolated from ~1 μg of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). RNA-seq libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770 ...
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bioRxiv - Developmental Biology 2021Quote: A ~5kb genomic sequence containing 2kb promoter and entire genomic region of tes-1 was PCR amplified using Phusion polymerase (NEB). The primers used were ...
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bioRxiv - Evolutionary Biology 2021Quote: Mutagenesis of the binding sites for the predicted Caudal and DEAF-1 transcription factors was performed sequentially with the Q5 Site-Directed Mutagenesis kit (New England Biolabs) taking as a template the pGreenRabbit vector containing the FBti0019386 sequence following manufacturer’s instructions (25) ...
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bioRxiv - Cell Biology 2020Quote: ... or using 400 U of T4 DNA ligase and 1X reaction buffer (50 mM Tris-HCl, 10 mM MgCl2 1 mM ATP, 10 mM DTT, New England Biolabs) at 16°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... 64 Annealed oligonucleotides were ligated with the AgeI-HF and EcoRI-HF digested pLKO.1 puro vector (Add gene #8453) using the T4 DNA Ligase (New England Biolabs) and the resulting plasmids were transformed into E ...
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bioRxiv - Cell Biology 2020Quote: ... Generation of double stranded cDNA and library construction were performed using NEBNext® mRNA Sample Prep Reagent Set 1 (NEB). Ligation of adapters was performed using Adapters prepared at the WTCHG according to the Illumina design (Multiplexing Sample Preparation Oliogonucleotide Kit) ...
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bioRxiv - Evolutionary Biology 2020Quote: Libraries for JU1373 and NIC58 were prepared simultaneously from mRNA isolated from 1 μg of pooled total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA fragmentation ...
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bioRxiv - Genomics 2021Quote: ... equal amount of naked DNA and DNA bound by Reb1 protein (~ 5pmol) were incubated with AAG (10 units) and APE1 (1 unit) (New England Biolabs) in a 20 μL reaction containing 1X Thermopol buffer (20mM Tris HCl pH 8.8 ...
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bioRxiv - Microbiology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1) (NEB #E7600) (New England Biolabs, Ipswich, MA). Libraries were pooled and sequenced by the Duke Sequencing and Genomic Technologies Shared Resource on an Illumina NextSeq 500 using the High-Output Kit to produce 75-basepair single-end reads.
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bioRxiv - Immunology 2020Quote: ... cDNA synthesised with SMARTNNN oligos were treated with 1 μl of Uracil DNA glycosylase (5 U/μl, New England Biolabs) and incubated for 15 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... rinsed gently whilst still in the slide tray three times with PBS and then treated with bleomycin (100 μM) in PBS or RNase H (100U.ml-1, NEB MO297S) in PBS supplemented with 0.1% triton ...
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bioRxiv - Microbiology 2020Quote: ... Gel extracted DNA fragments were incubated in a ratio of 1:3 of plasmid to insert (1:5 for inserts smaller than 300 nucleotides) in the presence of Gibson Assembly master mix (NEB) at 50°C for 1 hour.
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bioRxiv - Microbiology 2021Quote: ... harboring the p6- GLuc / p6 genomes or pTM-ORF1 vectors and epitope-tagged inserts were restricted by the suitable restriction enzymes (Table 1) and subsequently ligated using the T4 DNA ligase (NEB). Finally ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5–1 μg of circFL-seq cDNA was repaired and dA-tailed by the NEBNext FFPE DNA Repair Mix (NEB) and NEBNext Ultra II End repair/dA-tailing Module (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... A new plasmid was then created by ligating the PCR fragment and the linearized pETDuet-1 vector using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs).
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bioRxiv - Molecular Biology 2020Quote: ... The PCR reactions were pooled and treated for 1 hour at 37°C with 250 u /ml of Exonuclease I (NEB). Libraries were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) ...
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bioRxiv - Immunology 2020Quote: ... and ligation with the NotI digested PCR products was performed for 1 hour at room temperature with T4 DNA ligase (NEB). Colonies were screened by restriction digestion for the directional insertion of PCR products and the resulting lentiviral vectors were validated by Sanger sequencing.
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bioRxiv - Immunology 2021Quote: ... 5’ loxP site was added upstream of exon 1 using a galK cassette (NCI Frederick) that was PCR amplified with LongAmp Taq DNA polymerase (NEB), followed by recombineering-based gap repair using 5’ and 3’ arms of homology and cloning into PL253 (NCI Frederick ...
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bioRxiv - Molecular Biology 2021Quote: ... beads were resuspended in GRB buffer and incubated with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070 ...
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bioRxiv - Cell Biology 2021Quote: ... and ligated to linearized vector pGex-6p-1 (GST) or pET28-SUMO (His) also cut with the same restriction enzymes using T4 DNA ligase (NEB).