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Citations for New England Biolabs :
4351 - 4400 of 7437 citations for rno mir 155 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed according to the manufacturer’s instructions using Q5 Hot Start Polymerase (NEB) using the following program ...
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bioRxiv - Bioengineering 2020Quote: ... PCR 1 conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP2_KO_A and clpP2_KO_D primer pair (Table 3 ...
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bioRxiv - Biochemistry 2021Quote: ... The ligated fragments were amplified by PCR (Phusion polymerase, HF buffer, New England Biolabs) using the clpP1_A–clpP1D and clpP2_A– clpP2_CD primer pairs (Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... Erythromycin-sensitive strains were tested with colony PCR (OneTaq DNA polymerase, New England Biolabs) using the clpP_tag fwd and rev primer pair (Table 2 ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were constructed by ligating PCR products amplified with Q5 polymerase (New England Biolabs) using Golden Gate assembly (87) ...
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bioRxiv - Biochemistry 2020Quote: ... The template vector was removed from the PCR mixture by DpnI treatment (NEB, USA) at 37°C (1 – 2 h ...
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bioRxiv - Genomics 2021Quote: ... For other three libraries PCR products were digested using NheI restriction enzyme (NEB, R0131S) at 37 °C for 3 hours ...
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bioRxiv - Neuroscience 2020Quote: ... All PCR amplifications were performed using the Phusion® High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Cell Biology 2021Quote: ... The Gibson assembly principle [82] of incubating homologous PCR products with Taq ligase (NEB), T5 exonuclease (NEB ...
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bioRxiv - Neuroscience 2020Quote: Library inserts were generated by PCR using Phusion High Fidelity DNA polymerase (NEB Biolabs). The resulting HCDR3 library inserts were ligated into the base templates ...
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bioRxiv - Neuroscience 2020Quote: Library inserts were generated by PCR using Phusion High Fidelity DNA polymerase (NEB Biolabs). The resulting HCDR3 library inserts were ligated into the base templates ...
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bioRxiv - Neuroscience 2019Quote: ... the reannealed PCR products were digested with T7 endonuclease 1 (T7E1, New England Biolabs). Digestion was performed using 10 unites of T7E1 enzyme and incubated for 15 minutes at 37°C ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed by using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB). FGFR3 primers were:
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bioRxiv - Genetics 2021Quote: ... PCR was performed by using Q5 High-Fidelity DNA Polymerase (New England Biolabs, NEB). FGFR3 primers were:
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bioRxiv - Synthetic Biology 2021Quote: ... The entire plasmid was PCR amplified using Q5 DNA Polymerase (New England BioLabs Inc.) with the following degenerate primers where N indicates any base ...
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bioRxiv - Microbiology 2020Quote: ... dCas9 with PCR amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs) with specific primers (Table S1 ...
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bioRxiv - Systems Biology 2021Quote: ... Fragments for lentiviral vector construction were generated via PCR using Q5 polymerase (NEB M0491S) and primers specific to SV40NLS-mCherry-P2A and Gly-FT2DDD-KTR-mClover (Table S1 ...
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bioRxiv - Systems Biology 2021Quote: 25 ul - Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs)
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bioRxiv - Systems Biology 2020Quote: ... The resulting PCR products were digested for 1h at 37°C with DpnI (NEB) and transformed in competent Escherichia coli MC1061 cells ...
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bioRxiv - Neuroscience 2021Quote: ... Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic ...
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bioRxiv - Plant Biology 2021Quote: ... and i5 and i7 PCR amplification was done (Q5 polymerase; New England Biolabs, USA). Size selection and DNA purification were made using SPRI beads ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were analyzed on an agarose gel and HindIII Ladder (New England Biolabs), and subsequently purified with the GeneJet PCR Purification kit (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... Secondary amplification reactions were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) in 50 µL reaction (26 µL of 2X Mix ...
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bioRxiv - Genomics 2021Quote: ... ENGRAM plasmid and PCR product from above were digested with BsmBI (NEB, buffer 3.1) at 55°C for 1h and purified for ligation ...
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bioRxiv - Microbiology 2021Quote: ... All PCRs of flanking regions were carried out using Phusion-HF Master Mix (NEB) according to the manufacturer’s protocol with an annealing temperature (Ta ...
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bioRxiv - Molecular Biology 2021Quote: ... All DNA amplifications were conducted by PCR with Phusion High Fidelity DNA Polymerase (NEB) unless otherwise stated ...
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bioRxiv - Microbiology 2021Quote: ... The oligo was amplified in 25 individual low-cycle PCRs using Q5 polymerase (NEB).The amplicons were pooled and gel purified prior to cloning into a modified PASTN plasmid using 50 individual ligation reactions ...
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bioRxiv - Plant Biology 2020Quote: ... All PCRs were performed using Q5 High-Fidelity DNA Polymerase (New England Biolabs, USA), unless otherwise stated ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified PCR-product was recombined into the pDONR221 (ThermoFisher).The SUMO1SLR1 gene fusion was generated using high-fidelity PCR (Phusion DNA Polymerase; NEB) essentially as described in Atanassov et al ...
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bioRxiv - Microbiology 2020Quote: ... The RCA products were then purified using the Monarch PCR & DNA Cleanup Kit (NEB), then quantified using the Qubit dsDNA broad range assay ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were amplified using Q5® High fidelity DNA polymerase 2X master mix (NEB), 25 pmol of specific ultramers and 20 ng of DNA template ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μL PCR products were incubated with 1 μL DpnI enzyme (New England Biolabs) to clean up templates at 37 °C for 2.5 hours and then purified by gel-extraction following the manufacturer instructions (Cat# D2500-02 ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was end-repaired with T4 DNA polymerase (NEB; Cat. No. B02025), DNA Polymerase I ...
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bioRxiv - Cell Biology 2021Quote: ... Cloning PCRs were performed using Phusion High Fidelity DNA polymerase (M0530L, New England BioLabs) and then validated by Sanger sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... PCR amplification was done using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491). To amplify the transcript from the modified allele ...
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bioRxiv - Cell Biology 2021Quote: ... The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs) using forward primer OSV 389 and sequential reverse primers OSV 381 ...
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bioRxiv - Cancer Biology 2021Quote: ... PCRs were carried out using Q5 High-Fidelity DNA polymerase (New England Biolabs, #M0491) with an annealing temperature of 70°C ...
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bioRxiv - Cell Biology 2022Quote: ... all PCRs were performed using Q5 High Fidelity DNA polymerase (M0419; New England Biolabs) and restriction enzymes were from New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: DNA substrates for gel shift assays were amplified by PCR using Q5 Polymerase (NEB) and purified using a DNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Biochemistry 2022Quote: ... senC and egtD genes were PCR-amplified using Q5 High Fidelity DNA polymerase (NEB) with primers Vpa-SenC-F/R and Vpa-EgtD-F/R ...
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bioRxiv - Bioengineering 2022Quote: ... The insert and backbone were PCR amplified using Q5 polymerase (New England Biolabs (NEB) M0515 ...
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bioRxiv - Developmental Biology 2022Quote: ... 50 ng PCR product was incubated with 2 µL 10X NEBuffer 2 (NEB, B7002S) and nuclease-free water adding up to 19 µL using the following program ...
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bioRxiv - Bioengineering 2022Quote: ... The insert and backbone were PCR amplified using Q5 polymerase (New England Biolabs (NEB) M0515 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCRs were conducted with Phusion® High-Fidelity DNA Polymerase (New England Biolabs, M0530), and amplified DNA fragments were cloned via the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons were detected with Luna® Universal qRT-PCR Master Mix (New England Biolabs) in a CFXConnect Real-Time PCR Instrument (Bio-Rad Laboratories) ...
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bioRxiv - Genomics 2022Quote: ... 10ng of the library were amplified by PCR (Q5 high-fidelity DNA polymerase, NEB) for 12 cycles with primers annealing to the constant regions (primers MS_01-02 ...
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bioRxiv - Microbiology 2022Quote: ... and mreB genes were amplified by PCR with Phusion DNA polymerase (NEB, Ipswich, MA) using 10 ng C ...
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bioRxiv - Biochemistry 2022Quote: ... and the cDNA was amplified by PCR using Vent DNA polymerase (New England Biolabs). After DNA purification using the QIAEX II kit (QIAGEN) ...