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Citations for New England Biolabs :
4351 - 4400 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was collected and passed through a 2 mL Amylose resin (NEB E8021S) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse transcriptase linker was then ligated with T4 RNA Ligase 2 truncated (NEB) and reverse transcribed using M-MLV RNase H minus (Promega) ...
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bioRxiv - Biophysics 2021Quote: ... was generated by subcloning Mdn1 aa4381-4717 into pSNAP-tag(T7)-2 (NEB N9181S) downstream of the SNAP tag ...
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bioRxiv - Biochemistry 2022Quote: ... 2 and cloned into pET28a using T4 DNA ligase (New England Biolabs GmbH, Germany). The gene encoding ribose-phosphate pyrophosphokinase (RPPK ...
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bioRxiv - Bioengineering 2022Quote: ... Samples requiring dephosphorylation were treated with 2 U/μL λPP (New England Biolabs, # P0753) for 3 h at 30°C and then washed with PBSCM ...
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bioRxiv - Cancer Biology 2022Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclei were resuspended in 2 mL 1x NEBuffer 3.1 (New England Biolabs; B7203S) + 20uL NxGen RNase Inhibitor.
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bioRxiv - Molecular Biology 2022Quote: ... were ligated to dephosphorylated RNAs using T4 truncated RNA ligase 2 (K227Q) (NEB, #M0351L). Unligated linkers were depleted by using 5 U per sample of 5’ deadenylase (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
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bioRxiv - Microbiology 2022Quote: ... The resulting nucleic acids were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 µL of reactions were treated with 2 µL DNase I (NEB, Cat # M0303S) in a total volume of 100 µL at 37°C for ∼ 20 min before further analysis and treatment.
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bioRxiv - Immunology 2023Quote: ... and 2 µL of home-made Gibson mix (T5 exonuclease (0.2U; New England Biolabs), Phusion polymerase (12.5U ...
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bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction with T4 RNA ligase 2 was performed in 1x T4 Rnl2 (NEB) supplemented with PEG 8000 (10% ...
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bioRxiv - Microbiology 2023Quote: ... the RNA samples were treated using 2 units of DNase I (New England Biolabs) according to the manufacturer’s instructions to eliminate residual gDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... or NEBNext Ultra II DNA Library Prep Kit for Illumina (replicate 2: NEB, E7645S), according to the manufacturers’ protocols ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μL of 10x T4 DNA Ligase Buffer with 10 mM ATP (NEB, B0202S), 1 μL of PaqCI/AarI activator (5 pmol ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Cell Biology 2023Quote: ... The eluted sample was incubated with 2 ml amylose resin (New England Biolabs, E8021L) for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649), and the ratio of Ψ s versus uridines was determined via tandem quadrupole mass spectrometry (Supplementary Figure S2a).
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bioRxiv - Microbiology 2023Quote: ... 20-μL reactions contained 2 μM enzyme and 250 μM NTPs (New England BioLabs) in reaction buffer with 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... The annealed constructs were ligated using 2 µL T4 RNA ligase2 (NEB, 10U/µL), 3 µL 0.1% BSA (Ambion) ...
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bioRxiv - Molecular Biology 2023Quote: ... two pairs of oligonucleotides (Suppl. Table 2) were phosphorylated and ligated into BsbI (NEB) digested pDG459 plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... a pair of oligonucleotides (Suppl. Table 2) was phosphorylated and ligated into BsbI (NEB) digested pX459 and pX458 plasmids ...
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bioRxiv - Biophysics 2023Quote: ... 11 uL Exonuclease I Buffer and 2 uL Exonuclease I (New England Biolabs; M0293S) was added following reverse transcription and incubated at 37 °C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... Forked adapters (Supplementary Table S7) were annealed in NEBuffer 2 1X (NEB cat. B7002S) to a final concentration of 20 µM in a thermal cycler set to heat at 98°C and gradually cool down to 4°C with a 1°C per 20 sec gradient.
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bioRxiv - Genetics 2024Quote: ... the DNA-bound beads were digested with 2 μl BpmI (NEB, catalog number R0565L) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μM of dsDNA was incubated with CpG methyltransferase (M. SssI, New England Biolabs) in the presence of 200 μM of S-adenosylmethionine ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
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bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: ... 0.4 µL RNase Inhibitor and 2 µL Induro RT (200 U/µL; NEB M0681S) were incubated for 15 minutes at 60°C ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM MgCl2) supplemented with 1 mM ATP and 1 U/μL RNase Inhibitor (NEB). Reaction was incubated for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... pGEMHE plasmids were linearized and 5’-capped mRNA was synthesised with T7 polymerase (NEB HiScribeT7 ARCA kit) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified and desalted ligation product was then electroporated into high efficiency 5-alpha E.coli cells (NEB). The cells were grown overnight on LB-Carbenicillin plates and then ...
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bioRxiv - Genomics 2021Quote: ... 50 ng of DNA was digested using the 5-bp cutter ApeKI (New England Biolabs, Ipswich, MA) after which barcoded adapters were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... We mixed 20 µg of insert or 15 µg plasmid library with 5 µL AscI (NEB R0558L), 5 µL SbfI-HF (NEB R3642L) ...
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bioRxiv - Bioengineering 2021Quote: ... 5′ phosphorylation was performed either during synthesis (for tRNAfMet) or following synthesis using T4 Polynucleotide kinase (NEB)(for tRNALys and tRNAPhe ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions conducted at UR-MAI consisted of 50 μl reactions: 5 μl 10X Standard Taq Buffer (NEB), 2.5 μL of each primer ...
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bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
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bioRxiv - Biophysics 2020Quote: ... was added to the flow cell and incubated for 5 minutes followed by Nb.BsmI (0.2 U/μl) in 1x NEB 3.1 buffer (NEB) which was also incubated for 5 minutes ...