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Citations for New England Biolabs :
4251 - 4300 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
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bioRxiv - Genomics 2022Quote: ... 1 μl of Exonuclease 1 (NEB, M0293S) was added to each PCR product and incubated at 37C for 15 min to digest any single-stranded product ...
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bioRxiv - Molecular Biology 2024Quote: ... in 1× NEB buffer 1 (NEB, B7001S) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1% cholorophorm was added together with 10 μg mL-1 DNase 1 (NEB) and 1 μg mL-1 RNase A ...
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bioRxiv - Molecular Biology 2024Quote: ... The four PCR products were then assembled in a 1:1:1:1 ratio using HiFi assembly (NEB, E5520S) according to the manufacturer’s instructions to create the pLenti-STARR vector (pAS5018) ...
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bioRxiv - Biophysics 2021Quote: ... 72 °C for 5 minutes) using Phusion High-Fidelity PCR Master Mix with HF buffer (NEB), 0.1 µM of PE1.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10ul of PCR product was digested with 5 units of Msel (New England Biolabs, Ipswich, Massachusetts) for one hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Then every 5 μL of cleaned eluted product was redigested with 5μL of NotI-HF (NEB), 5 μL 10X CutSmart buffer (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... 5µl of the PCR mix was digested overnight with 5 units of BbsI (New England Biolabs) in 30 µl volume ...
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bioRxiv - Molecular Biology 2021Quote: ... The 5’-end of dephosphorylated mRNAs were then labeled using 10 units of T4 PNK (NEB) and 0.5 µl [γ-32P] ATP (6000 Ci/mmol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 μL of NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, Massachusetts, USA). Finally ...
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bioRxiv - Genomics 2020Quote: ... 5mM DTT) and phosphorylated the 5’-ends with 25 units of T4 Polynucleotide Kinase (NEB #M0201) at 37°C for 15 minutes ...
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bioRxiv - Systems Biology 2019Quote: ... 5 mM CaCl2 and 25% (v/v) glycerol)) containing MNase ((micrococcal nuclease (M0247S, New England Biolabs)) and supplemented with protease inhibitor ...
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bioRxiv - Microbiology 2019Quote: ... adjacent primers containing the mutation of interest were 5′-phosphorylated using T4 polynucleotide kinase (NEB, M0201S) using the buffer from the T4 DNA ligase ...
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bioRxiv - Genetics 2019Quote: ... and phosphate groups added to the 5’ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) by first incubating for 30 minutes without ATP and then 30 min with ATP to remove and add the phosphate group ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-fold molar access of pre-annealed T7 promoter adapters and either 80 units HindIII (NEB) or 40 units SpeI (NEB ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... We incubated 10 pmol gRNA with 5 pmol SauCas9 (New England Biolabs [NEB], Ipswich, MA, USA) at 25 °C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... 10 ml of T7 enzyme mix and 12.5 ml of 5’GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 ml for 30 min at 30°C ...
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bioRxiv - Immunology 2022Quote: ... 5 cycles of pre-amplification were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) with indexed primers (Ad1 and Ad2) ...
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bioRxiv - Microbiology 2020Quote: ... then ligated to total RNA extracted from CT cells using 5′ App DNA/RNA ligase (NEB). Next ...
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bioRxiv - Genetics 2022Quote: The unc-5 locus was amplified for sequencing by PCR using OneTaq 2x Master Mix (NEB) with primers DLO1081 (5’-TCTTTTCAGGCTTTGGCACTG-3’ ...
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bioRxiv - Molecular Biology 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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bioRxiv - Molecular Biology 2021Quote: ... a 5’ RACE RNA adapter was ligated to total Aag2 RNA with T4 RNA ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µg of the PCR product was subjected to Fragmentase® treatment (New England Biolab, NEB) until a smear of fragments was detected around 400-500pb by agarose gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... and were 5’ end-radiolabeled with [Ɣ-32P]-ATP using 10 U T4 polynucleotide kinase (NEB). Radiolabeled transcripts were purified on polyacrylamide gel (6% acrylamide:bisacrylamide 19:1 ...
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bioRxiv - Genetics 2019Quote: ... Thawed cells were resuspended in 5 ml of 1X NEB2 restriction enzyme buffer (New England Biolabs) and poured into a pre-chilled mortar containing liquid N2 ...
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bioRxiv - Biochemistry 2021Quote: Capping of RNA with the 5’-m7G cap was achieved using the Vaccinia Capping System (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the 5′ triphosphate was first removed by treatment with Quick CIP (New England BioLabs, manufacturer’s instructions). The reaction was then supplemented with 5 mM DTT and the same concentrations of T4 polynucleotide kinase (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... For each sample 250 ng of DNA was digested with 5 Units of RNase H (NEB) as control ...
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bioRxiv - Genomics 2021Quote: ... biotin was removed from the unligated ends by adding 5 µl of T4 DNA polymerase (NEB) to 5 ug of DNA ...
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bioRxiv - Genomics 2022Quote: ... a 5’ adapter was ligated onto the RNA transcript using T4 RNA Ligase I (NEB M0204L), and a third bead binding was performed ...
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bioRxiv - Genomics 2022Quote: ... a second bead binding was performed followed by a 5’ de-capping using RppH (NEB M0356S). The 5’ end of the transcript was phosphorylated using PNK (NEB M0201L ...
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bioRxiv - Microbiology 2022Quote: ... 5 μl of the cDNA was mixed with LongAmp Taq 2X Master Mix (New England Biolabs) and 100 pmol Sol-PrimerB (5’-GTTTCCCACTGGAGGATA-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µg of HMW DNA were de-phosphorylated using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30 min at 37°C followed by 5 min at 65°C ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA (5 µg in 20 µL ddH2O) was incubated with 2µL 100U Nuclease P1 (NEB) and 2 µL 10x Nuclease P1 buffer (NEB ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Genetics 2024Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a Phosphor Imager ...
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bioRxiv - Microbiology 2024Quote: ... or INRKKR cRNA/mRNA (5’-CATGATTGTGTCAACCTGCTCTG) were radioactively labelled using T4 polynucleotide kinase (New England Biolabs) and ɣ-32P-ATP (PerkinElmer) ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of gDNA was directly digested with 5 U of EcoRI-HF (New England Biolabs) in the ddPCR Supermix for Probes (no dUTPs ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Biophysics 2023Quote: ... Topoisomerase I treated DNA was purified using Monarch PCR & DNA Cleanup Kit (5 μg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... CX plasmid (Bukhari et al., 2016) and 5′UTR containing amplicon was digested with NheI (NEB) followed by ligation with T4DNA ligase (NEB) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... supplemented with 10 units of Exonuclease we and 5 units of Lambda Exonuclease (New England BioLabs), was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Library was PCR amplified for 5 cycles using the NEBNext High-Fidelity MasterMix (New England BioLabs) followed by qPCR amplification to determine the exact number of additional cycles required for optimal library amplification ...
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bioRxiv - Biochemistry 2023Quote: ... The HBB 5’ UTR containing mRNA was then capped using Vaccinia Capping enzyme (New England Biolabs) and 2′O-methylated using Vaccinia 2′O Methyltransferase (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Molecular Biology 2023Quote: ... we treated the RNase I RPFs with 5 U of T4 PNK (NEB, 10 U/µL) after RNA size selection [19] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 5 μM cell-impermeable SNAP-Surface Alexa Fluor 488 (New England Biolabs) in complete media (DMEM + 10% FBS ...