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Citations for New England Biolabs :
4301 - 4350 of 8151 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 5 μl of clean DNA was transformed into 50 μl of DH5α commercial competent cells (NEB). The whole transformation was spread onto a 10 cm ampicillin agar plate ...
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bioRxiv - Microbiology 2024Quote: ... was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB, #M0201S). Reverse transcription and PCR amplification of the library were followed by PAGE-assisted size selection were carried out as previously outlined67 ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: 10 µg of DNase-treated total RNA was digested with 5 U of RNase H (NEB) and 2 picomoles of a chimeric oligonucleotide targeting the MSRs for 20 minutes at 37 ℃ ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification of target sequences under 5 kb were produced using Q5 high fidelity polymerase (NEB # M0491S) under the recommended three-step amplification conditions ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Developmental Biology 2024Quote: ... Each qPCR reaction mixture contained 5 µL 2x Luna Universal qPCR master mix (New England BioLabs), 1 µL cDNA (2-fold dilution) ...
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bioRxiv - Immunology 2024Quote: ... the DNA was transformed into NEB® 5-alpha F’ I q chemically competent cells (NEB). Recombinants were selected on LB agar supplemented with 10 µg/ml of gentamicin ...
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bioRxiv - Genetics 2021Quote: ... 2 µL of Taq DNA polymerase (LongAmp Taq DNA Polymerase kit, New England Biolabs), 7.5 µL of dNTPs (dNTP set ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 hours at 55°C with 0.1 mg/ml Proteinase K (NEB P8107S).
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with NdeI (NEB) and AfeI (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... and then the extracted RNA was treated with 2 units of DNase I (NEB) and further cleaned up via phenol-chloroform (pH 4.3 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 μL of NEB Next High-Fidelity PCR Master Mix (2×) (NEB, Ipswich, USA), 1 μL of Universal PCR Primer (25 mmol ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2(genomic) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Biochemistry 2019Quote: ... then ligated to purified RNA species using T4 RNA Ligase 2 Truncated (NEB #M0242S) at 25°C for 1 h ...
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bioRxiv - Physiology 2019Quote: ... PCR was performed using Phusion Hot Start Flex 2× Master Mix (NEB, Frankfurt, Germany) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... was added to 2 pmol of pooled oligos by terminal transferase (New England Biolabs), oligos were labeled withAlexa647 NHS-ester (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... Barcoded pre-adenylated linkers were ligated using T4 RNA ligase 2 truncated K227Q (NEB) and rRNA was depleted using the Ribo-Zero gold kit (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were quenched with 2% H2O2 and digested in 10µg/ml Proteinase K (BioLabs). The reaction was stopped with 2mg/ml Glycine (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... The nuclei were washed two times with a plastid-lysis buffer (NEB + 2% triton) followed with centrifugation (5 min at 1500 g ...
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bioRxiv - Bioengineering 2020Quote: ... half of the DNA was mixed with 2 μL ExoI (M0293S, New England Biolabs), 1 μL of Lambda Exonuclease (M0262S ...
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bioRxiv - Bioengineering 2020Quote: ... 2 μL of PCR mixture was used for 10 μL KLD reaction (NEB, MA), which proceeded under room temperature for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... for level 2 reaction together with T4 DNA Ligase (New England BioLabs, Ipswich, UK). TCSn promoter DNA sequence (Zürcher et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µg DNA was treated with uracil DNA glycosylase (New England Biolabs, Inc.) at 37 °C for 60 min ...
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bioRxiv - Systems Biology 2021Quote: ... 2 micrograms of each library was digested with SphI-HF and SpeI-HF (NEB) and then treated with Antarctic phosphatase (NEB).
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bioRxiv - Systems Biology 2020Quote: ... Samples were digested using 2 Units/μL MNase in 20μL MNase Digestion Buffer (NEB) for 5 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 10 min with 2 mM Ribonucleoside-Vanadyl Complex RVC (New England Biolabs, S1402S) at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X with 2mM RVC ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... About 2-5μg of fragmented DNA was digested with 40U RNaseH (New England Biolabs) for at least 24 hours at 370C to serve as a negative control ...
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BstBI (NEB) and AfeI (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with 2 μM SNAP-Cell® 647-SiR (New England Biolabs) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg of the DNA substrate were digested with 30U of Nb.BbvCI (from NEB) for 2 h at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of 0.1 M Dithiothreitol (cat. n° M0368L, NEB, New England Biolabs, USA), 1 µL of 10 mM dNTP (cat ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of 0.1 M Dithiothreitol (cat. n° M0368L, NEB, New England Biolabs, USA), 1 µL of 10 mM dNTP (cat ...
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bioRxiv - Microbiology 2020Quote: ... The content was mixed with 2 μL of denaturing buffer (New England Biolabs™) and 2 μL of endo-β-N-acetylglucosaminidase-H (Endo-H ...
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bioRxiv - Neuroscience 2021Quote: ... Point mutants in the aman-2 cDNA were introduced by site-specific mutagenesis (NEB Q5 Site-Directed
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bioRxiv - Genetics 2019Quote: ... for 9 h and treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... Library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs). Library concentration was measured by DNA High Sensitivity Kit (Invitrogen ...
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bioRxiv - Systems Biology 2019Quote: ... the samples were added to 2 μL of second-strand synthesis mix (2.5× NEB buffer 2 [New England Biolabs] ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were labeled for 15 min with 2 μM TMR-Star (New England Biolabs) in complete medium for pulse labeling ...
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bioRxiv - Molecular Biology 2020Quote: ... PNK was used to dephosphorylate RNA for 2 hours (New England Biolabs, Ipswich, MA) followed by heat-inactivation ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: 2 µg of genomic DNA was digested with 100 units of MspI (NEB R0106M) or TaqαI (NEB R0149M ...
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bioRxiv - Genomics 2021Quote: ... 2 ul of the library was loaded using NEB loading dye (Cat: NEB #B7025) because it did not make a shadow on the gel as compared to the bromophenol blue dye ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was digested overnight (ON) with either 2 μl (100 U) Dpn II (NEB) (later experiments ...
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bioRxiv - Biochemistry 2020Quote: ... the DNA template was digested with 2 units of DNase I (New England Biolabs) for 1 h at 37 °C and the RNA was desalted twice by Illustra Microspin G-25 columns (GE Healthcare Life Sciences) ...
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bioRxiv - Developmental Biology 2020Quote: ... and then digested by 2 U of T7 endonuclease I (T7E1, New England BioLabs) at 37 °C for 15 min ...
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bioRxiv - Cell Biology 2020Quote: The pIGLR-2∷mCherry construct was generated with a Gibson assembly cloning kit (NEB) with the following four fragments ...