Labshake search
Citations for New England Biolabs :
4201 - 4250 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was degraded with 5 units of each RNase H (BioLabs cat #M029L) and RNase A/T1 (Thermo Scientific cat #EN0551) ...
-
bioRxiv - Genomics 2024Quote: ... 5 units of PolyA polymerase (E. coli or yeast as appropriate) (NEB#M0276S). The reaction was incubated at 37°C for either 7 or 30 minutes and stopped by purification with a MEGAclear column (Termofisher AM1908 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
-
bioRxiv - Bioengineering 2024Quote: ... phosphorylated at the 5’ ends with T4 polynucleotide kinase (New England Biolabs M0201), annealed to generate a double-stranded insert ...
-
bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
-
bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
-
bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
-
bioRxiv - Cancer Biology 2024Quote: ... The initial 5 cycles of PCR were performed using Q5 polymerase (NEB M0491), 200uM dNTPs (Invitrogen 10297-018 ...
-
bioRxiv - Cell Biology 2020Quote: ... the pHRRA1-GFP (pKS85) (Table 4) plasmid was PCR-amplified with Phusion HF Polymerase (NEB), using primers designed using the QuikChange Primer Design tool (Agilent) ...
-
bioRxiv - Immunology 2022Quote: The I53-50A and I53-50B.4.PT1 proteins26 were expressed in Lemo21(DE3) (NEB) in LB (10 g Tryptone ...
-
bioRxiv - Bioengineering 2020Quote: The following components comprised the RT-LAMP assay: 4 mM of MgSO4 (New England Biolabs), 1× final concentration of the isothermal amplification buffer (New England Biolabs) ...
-
bioRxiv - Genomics 2021Quote: ... the following were added into each reaction tube: 0.5 μL of 10x buffer 4 (NEB), 0.5 μL of 1 mg/mLbovine serum albumin solution (NEB) ...
-
bioRxiv - Immunology 2020Quote: The I53-50A and I53-50B.4.PT1 proteins were expressed in Lemo21(DE3) (NEB) in LB (10 g Tryptone ...
-
bioRxiv - Cancer Biology 2021Quote: ... the PCR product was incubated for 4 hours with 2uL DpnI (NEB, Cat. No R0176), at 37°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The second strand was synthesized with 4 U of T7 DNA polymerase (New England BioLabs) and purified with HighPrep PCR beads (MagBio) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the fragments were ligated over night at 4°C with T4 DNA Ligase (NEB) and heat shock transformed into DH5α competent E ...
-
bioRxiv - Genomics 2023Quote: ... and ligated for 4 hours using 2000 U T4 DNA ligase (New England Biolabs, M0202L). Subsequently ...
-
bioRxiv - Molecular Biology 2022Quote: ... 250ng of genomic DNA was digested with the 4-base cutter MnlI (NEB, Ipswich, MA), overnight at 37°C according to manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2023Quote: ... Frozen cell pellets were resuspended in 4 mL of IMAC buffer (NEB, Ipswich, MA, USA) on ice and dispersed using an ultrasonic disruptor (Sonics ...
-
bioRxiv - Microbiology 2022Quote: ... PCR master mix reagents (see Figure 4): Taq polymerase and 10X Reaction Buffer (NEB M0273S), nucleotide mix containing 10 mM dTTP ...
-
bioRxiv - Cancer Biology 2023Quote: ... A negative control treated for 4 hours at 37 °C with RNaseH1 (New England Biolabs) was included for each condition ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4 ug from each cell line was digested with 25 units EcoRI (New England Biolabs) in 100 ul total volume ...
-
bioRxiv - Genetics 2024Quote: ... homology arms were PCR amplified and cloned by a 4-fragment Gibson assembly (NEB E2621S) within the loxP-Blasticidin-HSVTK-loxP-TetOx96 and CuOx150-FRT-Neomycin-FRT plasmids73 ...
-
bioRxiv - Immunology 2024Quote: ... Purified RNA was treated with 4 units of DNase I (1µL/unit) (NEB, Cat. # M0303) for 1 hour at 37°C ...
-
bioRxiv - Pathology 2022Quote: ... Total RNA was subjected to two rounds of poly(A) selection using oligo-d(T)25 magnetic beads (New England Biolabs, CA, USA). Illumina compatible RNAseq library was prepared using NEB next ultra RNAseq library preparation kit ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted using TRIzol from 14 d old seedlings and poly(A) enriched before library preparation (NEB E7490 and E7765). Libraries were sequenced on Illumina Hiseq 2500 in a 2×100 bp format ...
-
bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
-
bioRxiv - Developmental Biology 2021Quote: ... To each of those tubes 5 μl of 10x CutSmart Buffer (New England Biolabs) and 2 μl of DpnI restriction enzyme (New England Biolabs ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was resuspended and treated with 7.5 units (1.5 µl) RNA 5′ pyrophosphohydrolase (NEB) for 1 h at 37°C in a total volume of 20 µl ...
-
bioRxiv - Cell Biology 2022Quote: ... and then unligated linker was digested away with 25 U Yeast 5’-deadenylase (NEB) and 5 U RecJ exonuclease (Epicentre) ...
-
bioRxiv - Immunology 2020Quote: ... Samples (20 μL) were loaded in DNA loading buffer (5 μL; New England Biolabs) and gels were run at 60 V for 120 mins.
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was treated with RppH (RNA 5’ Pyrophosphohydrolase, Art. No. M0356S, New England Biolabs) to dephosphorylate small RNAs and specifically enrich for 22G RNAs ...
-
bioRxiv - Genomics 2021Quote: ... 5 µL of 5X Phusion HF reaction buffer (all from New England Biolabs, USA), 0.5 µL of 10 mM premixed dNTPs and 0.5 µL of gDNA ...
-
bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: 5-10 μg RNA was diluted in 2x RNA loading dye (New England Biolabs) and size separated on 7 M Urea/15% polyacrylamide/0.5×TBE gels by gel electrophoresis ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
-
bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
-
bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
-
bioRxiv - Immunology 2020Quote: ... about 5 μg of the genomic DNA was digested with HaeIII (New England Biolabs) and purified using phenol:chloroform extraction followed by ethanol precipitation ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
-
bioRxiv - Molecular Biology 2020Quote: ... which was treated with 5 units of T7E1 (New England Biolabs, Ipswich,MA, USA) for 20 minutes at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... the RNP injection mix [5 µM EnGen® Spy Cas9 NLS (NEB Cat# M0646T) mixed with 2.5 µM of each gRNA and Cas9 buffer] was freshly prepared ...
-
bioRxiv - Zoology 2021Quote: ... we split the library and ran 5 high-fidelity Phusion (New England BioLabs, #M0530) PCRs for 12 cycles on a SimpliAmp thermal cycler (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... 400 ng of RNA was digested by 5 U of RppH (New England Biolabs) for 2 h at 37 °C ...
-
bioRxiv - Genomics 2020Quote: ... incubated for 5 minutes and magnetically separated using a Magnetic Separation Rack (NEB #S1509S). Three 1 mL 70% ethanol washes are performed on the Eppendorf tubes ...
-
bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
-
bioRxiv - Microbiology 2021Quote: ... dsRNA ladders and a 5’ Fluorescein-labeled RNA (300 nt) were provided by NEB and used as substrates for RNase I and RNase III activity assays ...
-
bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 μM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
-
bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...