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Citations for New England Biolabs :
351 - 382 of 382 citations for Pemetrexed disodium Impurity 30 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... NaCl 5M stock was added to reach 1 M final concentration along with 30 µl of Streptavidin magnetic beads (NEB # S1420S) for 2 hrs with end-over-end mixing at 4°C to capture biotinylated DNA fragments ...
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bioRxiv - Biochemistry 2024Quote: ... was added into each ligation mixture by incubation at 30 °C for 1hour followed by adding 1 µL RecJf (NEB, M0264L) for ssDNA digestion at 37°C for 1 hour ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reaction mixtures were treated with DNase I and incubated at 37 °C for 30 minutes before immediate purification (Monarch® RNA Cleanup Kit, NEB). A sample of each mRNA was reserved for quality analysis and transcripts were tailed with E ...
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bioRxiv - Synthetic Biology 2024Quote: ... All PCR reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Bioengineering 2020Quote: ... Addgene plasmid #44249)30 by site-specific mutation of 10A of dCas9 to 10D using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, US). Secondly ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 minutes in PBS containing the primary antibodies: rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs, Hangzhou, China), mouse monoclonal anti-tubulin β3 (801201 ...
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bioRxiv - Cancer Biology 2022Quote: ... Each plug was melted in 200 µl TE at 68 °C for 30 minutes and then digested using β-agarase I (BioLabs, M0392L) at 42 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A-tails were added by mixing the purified end-repaired DNA with dATPs and Klenow exonuclease and incubating at 37° C for 30 minutes (NEB, Ipswich, MA) and then purified using the Qiagen QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: PCR products (150 – 200 ng) were digested for 30 min at 37°C in 20 ul 1X restriction enzyme buffer (NEB buffer v3.1) containing 500 ng Cas9 (Alt-R® S.p ...
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bioRxiv - Microbiology 2021Quote: Vector pFLD was digested with PmlI at 37°C for 2 h and dephosphorylated with 5 U Antarctic phosphatase at 37 °C for 30 min as recommended by the manufacturer (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: TeloTags were prepared in 100μL reactions with 5mM of each of the 6 permutations of telomere splint Extended Data Fig.1A) and 30 mM of biotinylated adapter in HiFi Taq DNA Ligase Reaction Buffer (NEB, Cat. M0647S). Annealing was done by heating to 99 degrees and slowly decreasing the temperature 1°C /min in a Veriti™ 96-Well Thermal Cycler (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were digested in a 20 μl reaction with NEB Cutsmart buffer for 30 minutes - 1 hour with SmaI (NEB, Cat# R0141) to assess rDNA promoter methylation or with HpaII (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA was ligated for 4 h in a water bath at 16 °C (30 Weiss Units of T4 DNA Ligase, M0202 New England BioLabs® Inc). 300 μg of Proteinase K was used to reverse the cross-linking overnight at 65°C ...
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bioRxiv - Neuroscience 2024Quote: ... Sequence encoding the modified capsid insertion was amplified using serotype-specific primers with PCR cycles less than 30 by using Q5 2x Master Mix (NEB, Ipswich, MA). Two microliters of 1st round of PCR products were input to total 50 µl reaction of second round PCR to introduce Illumina i5 and i7 sequencing indices ...
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bioRxiv - Genomics 2024Quote: ... 50 ng crude digested pcDNA3.1 vector was combined with a 7-times molar excess of crude digested insert and incubated for 30 min at room temperature after addition of 1 µ L Quick Ligase (New England Biolabs, #M2200). NEB stable competent E ...
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bioRxiv - Molecular Biology 2020Quote: ... Each of the tubes were filled to 30 µL using 15 µL Q5 Hot Start High-Fidelity 2X Master Mix (NEB, #M0493S, Ispwich, MA), 1.5 µL of 20X Evagreen (Biotium ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were blocked with 5% BSA in 1X PBS for 30 minutes at room temperature followed by subsequent incubation with primary antibodies against MBP (NEB E8032L, 1:200) and RAD51 (Proteintech 14961-1-AP ...
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bioRxiv - Microbiology 2021Quote: ... Mung bean nuclease treatment was performed at 30°C for 30 min in 1x mung bean nuclease reaction buffer with 1U per reaction (Mung Bean Nuclease, M0250S, New England Biolabs, Ipswich, MA, USA). DNA (40 ng per sample ...
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bioRxiv - Cell Biology 2023Quote: We generated new expression constructs by SLIC (29, 30) and seamless cloning (NEBuilder® HiFi DNA Assembly, New England Biolabs, Cat. No. E2621S). For PCR amplification from plasmids or Bd genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... After 48 h cells were incubated for 30 min at 37 °C with SNAP-Cell 647-SiR substrate (NEB, final concentration 1.2 µM). Cells were then incubated for 30 min at 37 °C in fresh media to wash off unbound substrate ...
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bioRxiv - Molecular Biology 2024Quote: ... Template DNAs were removed by treating with DNase I at 37°C for 30 minutes and the IVT RNAs were further purified by RNA purification kit (NEB, cat. no. T2040L). The size of each IVT RNA was also confirmed by agarose gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... 1 µl of each enzyme cocktail component was added to the DNA sample and the digestion reaction was incubated at 37°C for 30 min in 1x rCutSmart Buffer (NEB, catalog number: E6004). Water was added to the untreated control for the digestion reaction ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Genetics 2022Quote: ... with insert-specific primers LC186 and 187) between hlh-30 and 3XFLAG-tag segments with HiFi cloning (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs Inc., Ipswich, MA). Lastly ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was kept on ice for 30 min followed by the addition of RNAse If (125 U/ml; NEB, Ipswich, MA; Cat#M0243L) and was allowed to incubate in a thermomixer set to 1200 RPM at 37°C for 3 min ...
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bioRxiv - Biochemistry 2024Quote: ... final concentration 30 nM) at 37 °C for 10 min before being added to M13mp18 single-stranded DNA (New England Biolabs, final concentration 25 ng/μL). Aliquots were quenched at the indicated time points by adding an equal volume of phenol-CHCl3-isoamyl alcohol and analyzed by agarose gel electrophoresis as described above.
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bioRxiv - Evolutionary Biology 2024Quote: ... Sequencing libraries were prepared with 30 ng of normalized DNA using the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, Massachusetts, USA). Custom 8 bp barcodes were added for pooling ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... initial digestion of DNA extracted from SDGC-SEC-purified stress granule cores was performed using exonuclease VIII (truncated, New England Biolabs; 20-30 units per μg of DNA) from 4 to 15 hours at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...